Methylation profiles of miR34 gene family in Vietnamese patients suffering from breast and lung cancers

  • Authors:
    • Vo Thi Thuong Lan
    • Ho Van Son
    • Vu Lan Trang
    • Nguyen Thu Trang
    • Nguyen Thi Phuong
    • Nguyen Linh Toan
    • Pham Anh Thuy Duong
  • View Affiliations

  • Published online on: June 18, 2018     https://doi.org/10.3892/mmr.2018.9182
  • Pages: 2476-2484
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Abstract

The three genes encoding small non‑coding microRNA (miR)34a, MIR34b and MIR34c act as tumor‑suppressor genes. Their aberrant expressions regulated by DNA methylation have been frequently found in various types of cancer. In the present study, the DNA promoter methylation profiles of the MIR34 gene family were analyzed using the methylation specific polymerase chain reaction in order to clarify their association with breast and lung cancer, non‑cancerous or normal adjacent tissues. The methylation frequency of MIR34a was significantly higher in breast cancer (49.37%) compared with normal adjacent tissues (30.38%). The methylation frequency of MIR34b/c was 59.49 and 62.03% in breast cancer and normal adjacent tissues, respectively. MIR34a methylation showed a significant concordance with that of MIR34b/c only in breast cancer tissue. MIR34a methylation was significantly associated with cancer and the invasive ductal carcinoma type of breast cancer (P=0.015 and P=0.02, respectively). Methylation frequency of MIR34a and MIR34b/c was 48.42 and 56.84% in lung cancer, and 47.22 and 51.39% in pulmonary diseases, respectively. No significant association was observed between the methylation status of MIR34a and MIR34b/c, and the clinicopathological features of lung cancer or with those of non‑cancerous pulmonary diseases. Promoter methylation of MIR34a and MIR34b/c occurs frequently and concomitantly in breast and lung cancer, as well as in pulmonary diseases tissues, but not in breast normal tissues adjacent to tumor. These results of the present study emphasize the involvement of MIR34 methylation in human diseases, including cancer. Furthermore, MIR34a methylation may be a promising marker for a subtype of breast cancer.
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August-2018
Volume 18 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lan VT, Son HV, Trang VL, Trang NT, Phuong NT, Toan NL and Duong PA: Methylation profiles of miR34 gene family in Vietnamese patients suffering from breast and lung cancers. Mol Med Rep 18: 2476-2484, 2018
APA
Lan, V.T., Son, H.V., Trang, V.L., Trang, N.T., Phuong, N.T., Toan, N.L., & Duong, P.A. (2018). Methylation profiles of miR34 gene family in Vietnamese patients suffering from breast and lung cancers. Molecular Medicine Reports, 18, 2476-2484. https://doi.org/10.3892/mmr.2018.9182
MLA
Lan, V. T., Son, H. V., Trang, V. L., Trang, N. T., Phuong, N. T., Toan, N. L., Duong, P. A."Methylation profiles of miR34 gene family in Vietnamese patients suffering from breast and lung cancers". Molecular Medicine Reports 18.2 (2018): 2476-2484.
Chicago
Lan, V. T., Son, H. V., Trang, V. L., Trang, N. T., Phuong, N. T., Toan, N. L., Duong, P. A."Methylation profiles of miR34 gene family in Vietnamese patients suffering from breast and lung cancers". Molecular Medicine Reports 18, no. 2 (2018): 2476-2484. https://doi.org/10.3892/mmr.2018.9182