Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2018 Volume 18 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2018 Volume 18 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Protective properties of spliced X box binding protein 1 in ozone‑induced spinal cord neuronal death

  • Authors:
    • Yun Li
    • Xu Zhao
    • Xiaowen Lin
    • Xuejun Zhao
    • Juntian Xie
    • Tao Sun
    • Zhijian Fu
  • View Affiliations / Copyright

    Affiliations: Department of Pain Management, Shandong Provincial Hospital Affiliated to Shandong University, Shandong University, Jinan, Shandong 250021, P.R. China
  • Pages: 2349-2355
    |
    Published online on: June 22, 2018
       https://doi.org/10.3892/mmr.2018.9212
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Administration of ozone (O3) is often used in the treatment of low back pain. Administration of O3 can, however, cause neurotoxicity in spinal cord neurons via induction of endoplasmic reticulum (ER) calcium (Ca2+) release and activation of the Ca2+/calmodulin‑dependent protein kinase II (CaMKII)/mitogen‑activated protein kinase (MAPK) pathway. The aim of the current study was to confirm whether administration of O3 causes ER stress and if the consequential overexpression of adenovirus‑mediated spliced X box binding protein 1 (XBP1s), which is the effector of ER stress and a crucial transcriptional factor gene in charge of cell survival, has a protective effect on spinal cord neurons after O3 exposure. To address this aim, the expression of GRP78, an ER chaperone and signaling regulator, and the expression of XBP1s in rat primary spinal cord neurons that underwent O3 exposure were investigated. Primary neurons exposed to O3 exhibited increased GRP78 and XBP1s expression levels. Interestingly, the effect of decreased neuron viability was blocked when cells were pretreated with Adv‑XBP1s. Moreover, overexpression of XBP1s suppressed cell death caused by O3 exposure. These results suggest that overexpression of activated XBP1s protects against neuronal cell death following O3 exposure and that activation of the XBP1s pathway may offer a preventative way for prophylactic treatment of spinal cord neurons exposed to O3.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Elvis AM and Ekta JS: Ozone therapy: A clinical review. J Nat Sci Biol Med. 2:66–70. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Paoloni M, Di Sante L, Cacchio A, Apuzzo D, Marotta S, Razzano M, Franzini M and Santilli V: Intramuscular oxygen-ozone therapy in the treatment of acute back pain with lumbar disc herniation: A multicenter, randomized, double-blind, clinical trial of active and simulated lumbar paravertebral injection. Spine (Phila Pa 1976). 34:1337–1344. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Zhang Y, Ma Y, Jiang J, Ding T and Wang J: Treatment of the lumbar disc herniation with intradiscal and intraforaminal injection of oxygen-ozone. J Back Musculoskelet Rehabil. 26:317–322. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Ginanneschi F, Cervelli C, Milani P and Rossi A: Ventral and dorsal root injury after oxygen-ozone therapy for lumbar disk herniation. Surg Neurol. 66:619–621. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Li Y, Lin X, Zhao X, Xie J, JunNan W, Sun T and Fu Z: Ozone (O3) elicits neurotoxicity in spinal cord neurons (SCNs) by inducing ER Ca(2+) release and activating the CaMKII/MAPK signaling pathway. Toxicol Appl Pharmacol. 280:493–501. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Hetz C: The unfolded protein response: controlling cell fate decisions under ER stress and beyond. Nat Rev Mol Cell Biol. 13:89–102. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Verkhratsky A and Petersen OH: The endoplasmic reticulum as an integrating signalling organelle: From neuronal signalling to neuronal death. Eur J Pharmacol. 447:141–154. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Tang CH, Chiu YC, Huang CF, Chen YW and Chen PC: Arsenic induces cell apoptosis in cultured osteoblasts through endoplasmic reticulum stress. Toxicol Appl Pharmacol. 241:173–181. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Zhang K and Kaufman RJ: Signaling the unfolded protein response from the endoplasmic reticulum. J Biol Chem. 279:25935–25938. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Lin JH, Li H, Yasumura D, Cohen HR, Zhang C, Panning B, Shokat KM, Lavail MM and Walter P: IRE1 signaling affects cell fate during the unfolded protein response. Science. 318:944–949. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Paschen W, Aufenberg C, Hotop S and Mengesdorf T: Transient cerebral ischemia activates processing of xbp1 messenger RNA indicative of endoplasmic reticulum stress. J Cereb Blood Flow Metab. 23:449–461. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Lu PD, Harding HP and Ron D: Translation reinitiation at alternative open reading frames regulates gene expression in an integrated stress response. J Cell Biol. 167:27–33. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Yoshida H, Yanagi H, Yura T and Mori K: Identification of the cis-acting endoplasmic reticulum stress response element responsible for transcriptional induction of mammalian glucose-regulate proteins. Involvement of basic leucine zipper transcription factors. J Biol Chem. 273:33741–33749. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Yoshida H: Unconventional splicing of XBP-1 mRNA in the unfolded protein response. Antioxid Redox Signal. 9:2323–33. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Chen J, Qin J, Liu X, Han Y, Yang Z, Chang X and Ji X: Nitric oxide-mediated neuronal apoptosis in rats with recurrent febrile seizures through endoplasmic reticulum stress pathway. Neurosci Lett. 443:134–139. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Marsala M, Kakinohana O, Hefferan MP, Cizkova D, Kinjoh K and Marsala S: Synaptogenesis and amino acid release from long term embryonic rat spinal cord neuronal culture using tissue culture inserts. J Neurosci Methods. 141:21–27. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Ahmad A, Ahmad S, Chang LY, Schaack J and White CW: Endothelial Akt activation by hyperoxia: Role in cell survival. Free Radic Biol Med. 40:1108–1118. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Muto M, Ambrosanio G, Guarnieri G, Capobianco E, Piccolo G, Annunziata G and Rotondo A: Low back pain and sciatica: Treatment with intradiscal-intraforaminal O (2)-O (3) injection. Our experience. Radiol Med. 113:695–706. 2008.(In English, Italian). View Article : Google Scholar : PubMed/NCBI

19 

Yoshida H, Matsui T, Yamamoto A, Okada T and Mori K: XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor. Cell. 107:881–891. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Lee AE, Iwakoshi NN and Glimcher LH: XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response. Mol Cell Biol. 23:7448–7459. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Oyadomari S and Mori M: Roles of CHOP/GADD153 in endoplasmic reticulum stress. Cell Death Differ. 11:381–389. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Mihailidou C, Papazian I, Papavassiliou AG and Kiaris H: CHOP-dependent regulation of p21/waf1 during ER stress. Cell Physiol Biochem. 25:761–766. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Yamauchi T, Sakurai M, Abe K, Matsumiya G and Sawa Y: Impact of the endoplasmic reticulum stress response in spinal cord after transient ischemia. Brain Res. 1169:24–33. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Ron D and Walter P: Signal integration in the endoplasmic reticulum unfolded protein response. Nat Rev Mol Cell Biol. 8:519–529. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Sado M, Yamasaki Y, Iwanaga T, Onaka Y, Ibuki T, Nishihara S, Mizuguchi H, Momota H, Kishibuchi R, Hashimoto T, et al: Protective effect against Parkinson's disease-related insults through the activation of XBP1. Brain Res. 1257:16–24. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Ibuki T, Yamasaki Y, Mizuguchi H and Sokabe M: Protective effects of XBP1 against oxygen and glucose deprivation/reoxygenation injury in rat primary hippocampal neurons. Neurosci Lett. 518:45–48. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Tian PG, Jiang ZX, Li JH, Zhou Z and Zhang QH: Spliced XBP1 promotes macrophage survival and autophagy by interacting with Beclin-1. Biochem Biophys Res Commun. 463:518–523. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li Y, Zhao X, Lin X, Zhao X, Xie J, Sun T and Fu Z: Protective properties of spliced X box binding protein 1 in ozone‑induced spinal cord neuronal death. Mol Med Rep 18: 2349-2355, 2018.
APA
Li, Y., Zhao, X., Lin, X., Zhao, X., Xie, J., Sun, T., & Fu, Z. (2018). Protective properties of spliced X box binding protein 1 in ozone‑induced spinal cord neuronal death. Molecular Medicine Reports, 18, 2349-2355. https://doi.org/10.3892/mmr.2018.9212
MLA
Li, Y., Zhao, X., Lin, X., Zhao, X., Xie, J., Sun, T., Fu, Z."Protective properties of spliced X box binding protein 1 in ozone‑induced spinal cord neuronal death". Molecular Medicine Reports 18.2 (2018): 2349-2355.
Chicago
Li, Y., Zhao, X., Lin, X., Zhao, X., Xie, J., Sun, T., Fu, Z."Protective properties of spliced X box binding protein 1 in ozone‑induced spinal cord neuronal death". Molecular Medicine Reports 18, no. 2 (2018): 2349-2355. https://doi.org/10.3892/mmr.2018.9212
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Zhao X, Lin X, Zhao X, Xie J, Sun T and Fu Z: Protective properties of spliced X box binding protein 1 in ozone‑induced spinal cord neuronal death. Mol Med Rep 18: 2349-2355, 2018.
APA
Li, Y., Zhao, X., Lin, X., Zhao, X., Xie, J., Sun, T., & Fu, Z. (2018). Protective properties of spliced X box binding protein 1 in ozone‑induced spinal cord neuronal death. Molecular Medicine Reports, 18, 2349-2355. https://doi.org/10.3892/mmr.2018.9212
MLA
Li, Y., Zhao, X., Lin, X., Zhao, X., Xie, J., Sun, T., Fu, Z."Protective properties of spliced X box binding protein 1 in ozone‑induced spinal cord neuronal death". Molecular Medicine Reports 18.2 (2018): 2349-2355.
Chicago
Li, Y., Zhao, X., Lin, X., Zhao, X., Xie, J., Sun, T., Fu, Z."Protective properties of spliced X box binding protein 1 in ozone‑induced spinal cord neuronal death". Molecular Medicine Reports 18, no. 2 (2018): 2349-2355. https://doi.org/10.3892/mmr.2018.9212
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team