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Article

Aclidinium bromide inhibits the growth and metastasis of gastric cancer MKN‑28 cells via the PI3K signaling pathway

  • Authors:
    • Yuanzhi Wang
    • Ping Cui
    • Jingjing Liu
    • Hongxia Wu
    • Jun Ma
  • View Affiliations / Copyright

    Affiliations: Department of Operating, Binzhou Central Hospital, Binzhou, Shandong 251700, P.R. China, Department of Oncology, Binzhou City TB Prevention and Control of Hospital, Binzhou, Shandong 251700, P.R. China, Department of Neurosurgery, Binzhou Central Hospital, Binzhou, Shandong 251700, P.R. China, Department of Nursing, Binzhou Central Hospital, Binzhou, Shandong 251700, P.R. China, Department of Infectious Diseases, Binzhou City TB Prevention and Control of Hospital, Binzhou, Shandong 251700, P.R. China
  • Pages: 2263-2268
    |
    Published online on: June 25, 2018
       https://doi.org/10.3892/mmr.2018.9220
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Abstract

The present study investigated the effect and underling mechanisms of aclidinium bromide, a novel, inhaled long‑acting muscarinic antagonist, on the development of gastric cancer. Human gastric cancer MKN‑28 cells, as a model in vitro, were treated with aclidinium bromide and dimethyl sulfoxide. Cell Counting Kit‑8 assay, transwell assay and flow cytometry were used to assess cell proliferation, invasion/migration and apoptosis, respectively. In addition, western blotting was performed to determine the relative expression of proteins associated with apoptosis and the phosphatidylinositol‑3‑kinase (PI3K) signaling pathway. Optical density values of MKN‑28 cells were decreased in a time‑ and dose‑dependent manner in the aclidinium bromide treated group. Matrigel invasion analysis demonstrated the number of invasive cells were significantly decreased in the aclidinium bromide‑treated group when compared with the control group. Furthermore, aclidinium bromide led to the marked reduction of the number of MKN‑28 cells passing though the microwells of the transwell chamber. The expression levels of the anti‑apoptotic protein B‑cell lymphoma 2 (Bcl‑2) decreased, and the expression of pro‑apoptotic proteins active Caspase3 and Bcl‑2‑associated X protein increased concurrently following aclidinium bromide stimulation using western blotting. The phosphorylation of protein kinase B and mechanistic target of rapamycin were significantly inhibited in MKN‑28 cells treated with aclidinium bromide; and the activity of the downstream proteins such as p70S6K and Cyclin D1 were also significantly decreased. In conclusion, aclidinium bromide could inhibit gastric cancer cell proliferation and metastasis, which may be associated with the enhancement of apoptosis induced by the PI3K signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Cui P, Liu J, Wu H and Ma J: Aclidinium bromide inhibits the growth and metastasis of gastric cancer MKN‑28 cells via the PI3K signaling pathway. Mol Med Rep 18: 2263-2268, 2018.
APA
Wang, Y., Cui, P., Liu, J., Wu, H., & Ma, J. (2018). Aclidinium bromide inhibits the growth and metastasis of gastric cancer MKN‑28 cells via the PI3K signaling pathway. Molecular Medicine Reports, 18, 2263-2268. https://doi.org/10.3892/mmr.2018.9220
MLA
Wang, Y., Cui, P., Liu, J., Wu, H., Ma, J."Aclidinium bromide inhibits the growth and metastasis of gastric cancer MKN‑28 cells via the PI3K signaling pathway". Molecular Medicine Reports 18.2 (2018): 2263-2268.
Chicago
Wang, Y., Cui, P., Liu, J., Wu, H., Ma, J."Aclidinium bromide inhibits the growth and metastasis of gastric cancer MKN‑28 cells via the PI3K signaling pathway". Molecular Medicine Reports 18, no. 2 (2018): 2263-2268. https://doi.org/10.3892/mmr.2018.9220
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Cui P, Liu J, Wu H and Ma J: Aclidinium bromide inhibits the growth and metastasis of gastric cancer MKN‑28 cells via the PI3K signaling pathway. Mol Med Rep 18: 2263-2268, 2018.
APA
Wang, Y., Cui, P., Liu, J., Wu, H., & Ma, J. (2018). Aclidinium bromide inhibits the growth and metastasis of gastric cancer MKN‑28 cells via the PI3K signaling pathway. Molecular Medicine Reports, 18, 2263-2268. https://doi.org/10.3892/mmr.2018.9220
MLA
Wang, Y., Cui, P., Liu, J., Wu, H., Ma, J."Aclidinium bromide inhibits the growth and metastasis of gastric cancer MKN‑28 cells via the PI3K signaling pathway". Molecular Medicine Reports 18.2 (2018): 2263-2268.
Chicago
Wang, Y., Cui, P., Liu, J., Wu, H., Ma, J."Aclidinium bromide inhibits the growth and metastasis of gastric cancer MKN‑28 cells via the PI3K signaling pathway". Molecular Medicine Reports 18, no. 2 (2018): 2263-2268. https://doi.org/10.3892/mmr.2018.9220
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