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Article Open Access

MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10

  • Authors:
    • Jinhui Liu
    • Yi Jiang
    • Yicong Wan
    • Shulin Zhou
    • Sunita Thapa
    • Wenjun Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2661-2668
    |
    Published online on: July 5, 2018
       https://doi.org/10.3892/mmr.2018.9252
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Abstract

Ovarian cancer is the most lethal gynecological cancer and its metastasis leads to a poor prognosis. The present study was designed to elucidate how microRNA (miR)‑665 regulates the proliferation and migration of ovarian tumor cells. Reverse transcription‑polymerase chain reaction (RT‑PCR) demonstrated that miR‑665 expression was decreased in ovarian cancer tissues. Increased expression of miR‑665 suppressed the growth and migration of ovarian cancer cells, whereas the downregulated expression of miR‑665 led to the opposite results. Bioinformatics tools identified homeobox A10 (HOXA10) as a target of miR‑665. Following miR‑665 overexpression, HOXA10 protein expression was significantly reduced. A dual luciferase assay revealed that miR‑665 bound to the 3'‑untranslated region of HOXA10. Immunohistochemistry and RT‑PCR revealed that the expression of HOXA10 was negatively correlated with the expression of miR‑665. It was concluded that miR‑665 targets HOXA10 and may act as a tumor‑suppressing gene in ovarian cancer. This pathway may be involved in the development and metastasis of ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Jiang Y, Wan Y, Zhou S, Thapa S and Cheng W: MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10. Mol Med Rep 18: 2661-2668, 2018.
APA
Liu, J., Jiang, Y., Wan, Y., Zhou, S., Thapa, S., & Cheng, W. (2018). MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10. Molecular Medicine Reports, 18, 2661-2668. https://doi.org/10.3892/mmr.2018.9252
MLA
Liu, J., Jiang, Y., Wan, Y., Zhou, S., Thapa, S., Cheng, W."MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10". Molecular Medicine Reports 18.3 (2018): 2661-2668.
Chicago
Liu, J., Jiang, Y., Wan, Y., Zhou, S., Thapa, S., Cheng, W."MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10". Molecular Medicine Reports 18, no. 3 (2018): 2661-2668. https://doi.org/10.3892/mmr.2018.9252
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Jiang Y, Wan Y, Zhou S, Thapa S and Cheng W: MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10. Mol Med Rep 18: 2661-2668, 2018.
APA
Liu, J., Jiang, Y., Wan, Y., Zhou, S., Thapa, S., & Cheng, W. (2018). MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10. Molecular Medicine Reports, 18, 2661-2668. https://doi.org/10.3892/mmr.2018.9252
MLA
Liu, J., Jiang, Y., Wan, Y., Zhou, S., Thapa, S., Cheng, W."MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10". Molecular Medicine Reports 18.3 (2018): 2661-2668.
Chicago
Liu, J., Jiang, Y., Wan, Y., Zhou, S., Thapa, S., Cheng, W."MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10". Molecular Medicine Reports 18, no. 3 (2018): 2661-2668. https://doi.org/10.3892/mmr.2018.9252
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