Open Access

Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction

  • Authors:
    • Yuhong Dou
    • Yuxia Li
    • Caifeng Ma
    • Huijun Zhu
    • Jikun Du
    • Helu Liu
    • Qiong Liu
    • Rui Chen
    • Ying Tan
  • View Affiliations

  • Published online on: July 5, 2018     https://doi.org/10.3892/mmr.2018.9253
  • Pages: 2889-2897
  • Copyright : © Dou et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Human adenovirus (HAdV) is increasingly recognized as a major cause of human respiratory tract viral infections. Its outbreaks and epidemics in various populations resulted in considerable morbidity and mortality. Therefore, a rapid and specific assay for HAdV in clinical samples is of crucial importance to diagnosing HAdV infections. The present study aimed to develop and evaluate a multiplex quantitative polymerase chain reaction (qPCR) assay for the rapid detection and accurate quantification of HAdV B, C and E. The lower limit of detection for this assay was two genomic copies per reaction, and quantitative linearity ranged from 2 to 2x106 copies per reaction of the input viral DNA. Furthermore, 3,160 throat swab samples that tested HAdV negative by the immunofluorescence assay were collected and retested using the multiplex qPCR assay. The results showed that 2,906 samples were HAdV negative and the other 254 samples were HAdV positive. The HAdV species identified included B (184 samples), C (51 samples), and E (39 samples). Among the three HAdV species, HAdV B and E were detected from 8 samples, and HAdV C and E were detected from other 12 samples. The overall results demonstrated that the sensitivity and specificity of the proposed assay were 100% (254/254) and 99.6% (2894/2906), respectively. From the perspective of routine clinical diagnosis, this assay represented a rapid (≤1.5 h) and economic strategy, and had the potential to be used for the rapid and accurate diagnosis of human respiratory infections caused by HAdV B, C and E.
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September-2018
Volume 18 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Dou Y, Li Y, Ma C, Zhu H, Du J, Liu H, Liu Q, Chen R and Tan Y: Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction. Mol Med Rep 18: 2889-2897, 2018
APA
Dou, Y., Li, Y., Ma, C., Zhu, H., Du, J., Liu, H. ... Tan, Y. (2018). Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction. Molecular Medicine Reports, 18, 2889-2897. https://doi.org/10.3892/mmr.2018.9253
MLA
Dou, Y., Li, Y., Ma, C., Zhu, H., Du, J., Liu, H., Liu, Q., Chen, R., Tan, Y."Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction". Molecular Medicine Reports 18.3 (2018): 2889-2897.
Chicago
Dou, Y., Li, Y., Ma, C., Zhu, H., Du, J., Liu, H., Liu, Q., Chen, R., Tan, Y."Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction". Molecular Medicine Reports 18, no. 3 (2018): 2889-2897. https://doi.org/10.3892/mmr.2018.9253