Lycium barbarum polysaccharides protect human trophoblast HTR8/SVneo cells from hydrogen peroxide‑induced oxidative stress and apoptosis

Li,Jing; Ding,Zhongjun; Yang,Yue; Mao,Baohong; Wang,Yanxia; Xu,Xiaoying

doi:10.3892/mmr.2018.9274

Lycium barbarum polysaccharides protect human trophoblast HTR8/SVneo cells from hydrogen peroxide‑induced oxidative stress and apoptosis

Authors:
- Jing Li
- Zhongjun Ding
- Yue Yang
- Baohong Mao
- Yanxia Wang
- Xiaoying Xu
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Affiliations: Department of Women and Children's Medical Center, Gansu Provincial Maternity and Child Care Hospital, Lanzhou, Gansu 730050, P.R. China, Reproduction Medicine Center, Gansu Provincial Maternity and Child Care Hospital, Lanzhou, Gansu 730050, P.R. China, Discipline of Physiology, Gansu University of Chinese Medicine, Lanzhou, Gansu 730000, P.R. China, Perinatal Center, Gansu Provincial Maternity and Child Care Hospital, Lanzhou, Gansu 730050, P.R. China
Published online on: July 12, 2018 https://doi.org/10.3892/mmr.2018.9274
Pages: 2581-2588
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pregnancy complications are associated with abnormal cytotrophoblast differentiation and invasion. Hydrogen peroxide (H2O2) is an important mediator of oxidative ischemia/reperfusion stress in the placenta. Lycium barbarum polysaccharides (LBP) have been demonstrated to counteract oxidative free radicals. The effects of LBP in trophoblast HTR8/SVneo cells injured with H2O2 were examined. A cell counting kit‑8 assay was performed to detect the effect of LBP at different concentrations on the proliferative ability of H2O2 injured trophoblast cells. Flow cytometry was used to determine the levels of reactive oxygen species (ROS), mitochondria membrane potential (MMP) disruption and apoptosis. Superoxide dismutase (SOD) activity and lactate dehydrogenase (LDH) leakage into the supernatant was detected by ultraviolet spectrophotometry. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were performed to detect the expression of apoptosis‑associated factors, including survivin, hypoxia inducible factor 1‑α (HIF1‑α), Bcl‑2 apoptosis regulator (Bcl‑2), Bcl‑2 associated X apoptosis regulator (Bax). The results revealed that LBP protected the proliferative ability of trophoblast cells injured with H2O2 in a dose‑dependent manner. LBP inhibited the oxidative stress induced by H2O2, by reducing ROS and LDH levels and increasing SOD activity. Additionally, LBP decreased MMP disruption and cell apoptosis induced by H2O2, by increasing the mRNA and protein expression of survivin, HIF1‑α and Bcl‑2 and decreasing Bax expression. Therefore, it was concluded that LBP protected human trophoblast cells from H2O2‑induced oxidative stress and cell apoptosis via regulation of apoptosis‑associated factor expression. It will provide a novel strategy for the treatment of pregnancy complications.

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