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Article Open Access

Integrative analysis of promising molecular biomarkers and pathways for coronary artery disease using WGCNA and MetaDE methods

  • Authors:
    • Shilin Yan
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    Affiliations: Department of Cardiology, Yangling Demonstration Zone Hospital, Xianyang, Shaanxi 712100, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2789-2797
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    Published online on: July 16, 2018
       https://doi.org/10.3892/mmr.2018.9277
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Abstract

The present study aimed to examine the molecular mechanisms of coronary artery disease (CAD). A total of four microarray datasets (training dataset no. GSE12288; validation dataset nos. GSE20680, GSE20681 and GSE42148) were downloaded from the Gene Expression Omnibus database, which included CAD and healthy samples. Weighted gene co‑expression network analysis was applied to identify highly preserved modules across the four datasets. Differentially expressed genes (DEGs) with significant consistency in the four datasets were selected using the MetaDE method. The overlapping genes amongst the DEGs with significant consistency and in the preserved modules were used to construct a protein‑protein interaction (PPI) network, followed by functional enrichment analysis. A total of 11 modules were established in the training dataset, and five of them were highly preserved across all four datasets, including 873 genes. There was a total of 836 DEGs with significant consistency in the four datasets. A total of 177 overlapping genes were selected, with which a PPI network was constructed. The top five genes of the PPI network were identified based on their degrees: LCK proto‑oncogene, Src family tyrosine kinase (LCK), euchromatic histone lysine methyltransferase 2 (EHMT2), inosine monophosphate dehydrogenase 2 (IMPDH2), protein phosphatase 4 catalytic subunit (PPP4C) and ζ‑chain of T‑cell receptor associated protein kinase 70 (ZAP70). Genes in the PPI network were significantly involved in a number of Kyoto Encyclopedia Genes and Genomes pathways, including the ‘natural killer cell mediated cytotoxicity’, ‘primary immunodeficiency’ and ‘Fc gamma R‑mediated phagocytosis’ pathways. LCK, EHMT2, IMPDH2, PPP4C and ZAP70 are suggested as promising molecular biomarkers for CAD. The ‘natural killer cell mediated cytotoxicity’, ‘primary immunodeficiency’ and ‘Fc gamma R‑mediated phagocytosis’ pathways may serve important roles in CAD.
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Copy and paste a formatted citation
Spandidos Publications style
Yan S: Integrative analysis of promising molecular biomarkers and pathways for coronary artery disease using WGCNA and MetaDE methods. Mol Med Rep 18: 2789-2797, 2018.
APA
Yan, S. (2018). Integrative analysis of promising molecular biomarkers and pathways for coronary artery disease using WGCNA and MetaDE methods. Molecular Medicine Reports, 18, 2789-2797. https://doi.org/10.3892/mmr.2018.9277
MLA
Yan, S."Integrative analysis of promising molecular biomarkers and pathways for coronary artery disease using WGCNA and MetaDE methods". Molecular Medicine Reports 18.3 (2018): 2789-2797.
Chicago
Yan, S."Integrative analysis of promising molecular biomarkers and pathways for coronary artery disease using WGCNA and MetaDE methods". Molecular Medicine Reports 18, no. 3 (2018): 2789-2797. https://doi.org/10.3892/mmr.2018.9277
Copy and paste a formatted citation
x
Spandidos Publications style
Yan S: Integrative analysis of promising molecular biomarkers and pathways for coronary artery disease using WGCNA and MetaDE methods. Mol Med Rep 18: 2789-2797, 2018.
APA
Yan, S. (2018). Integrative analysis of promising molecular biomarkers and pathways for coronary artery disease using WGCNA and MetaDE methods. Molecular Medicine Reports, 18, 2789-2797. https://doi.org/10.3892/mmr.2018.9277
MLA
Yan, S."Integrative analysis of promising molecular biomarkers and pathways for coronary artery disease using WGCNA and MetaDE methods". Molecular Medicine Reports 18.3 (2018): 2789-2797.
Chicago
Yan, S."Integrative analysis of promising molecular biomarkers and pathways for coronary artery disease using WGCNA and MetaDE methods". Molecular Medicine Reports 18, no. 3 (2018): 2789-2797. https://doi.org/10.3892/mmr.2018.9277
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