Open Access

miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE

  • Authors:
    • Chonggao Yin
    • Guoxin Zhang
    • Ruimei Sun
    • Xinting Pan
    • Xuewen Wang
    • Hongli Li
    • Yunbo Sun
  • View Affiliations

  • Published online on: July 16, 2018     https://doi.org/10.3892/mmr.2018.9294
  • Pages: 2621-2630
  • Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In our previous study, advanced glycosylation end‑product specific receptor (RAGE) was observed to bind to S100A8/A9 and cause epithelial mesenchymal transition (EMT). The results from target gene prediction revealed that microRNA (miR)‑185‑5p had a RAGE binding site. However, the function of miR‑185‑5p in the invasion and migration of breast cancer remains ambiguous. In the present study, the expression of miR‑185‑5p was examined in breast cancer tissues and cells. Clinical features revealed a negative correlation between miR‑185‑5p and tumor size, as well as in tumor differentiation and lymph node metastasis in breast cancer. In addition, miR‑185‑5p was negatively associated with RAGE, and this miRNA reversed the EMT of breast cancer by modulating RAGE in vitro. In addition, miR‑185‑5p inhibited the S100A8/A9‑induced EMT of breast cancer cells by the nuclear factor‑κB/Snail signaling pathway. Notably, miR‑185‑5p upregulation inhibited the F‑actin polymerization induced by S100A8/A9 in breast cancer. Furthermore, overexpression of miR‑185‑5p and reduction of RAGE inhibited lung metastasis node in vivo. Thus, miR‑185‑5p represents a potential therapeutic target in breast cancer by modulating RAGE.
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September-2018
Volume 18 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yin C, Zhang G, Sun R, Pan X, Wang X, Li H and Sun Y: miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE. Mol Med Rep 18: 2621-2630, 2018
APA
Yin, C., Zhang, G., Sun, R., Pan, X., Wang, X., Li, H., & Sun, Y. (2018). miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE. Molecular Medicine Reports, 18, 2621-2630. https://doi.org/10.3892/mmr.2018.9294
MLA
Yin, C., Zhang, G., Sun, R., Pan, X., Wang, X., Li, H., Sun, Y."miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE". Molecular Medicine Reports 18.3 (2018): 2621-2630.
Chicago
Yin, C., Zhang, G., Sun, R., Pan, X., Wang, X., Li, H., Sun, Y."miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE". Molecular Medicine Reports 18, no. 3 (2018): 2621-2630. https://doi.org/10.3892/mmr.2018.9294