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Article Open Access

miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE

  • Authors:
    • Chonggao Yin
    • Guoxin Zhang
    • Ruimei Sun
    • Xinting Pan
    • Xuewen Wang
    • Hongli Li
    • Yunbo Sun
  • View Affiliations / Copyright

    Affiliations: College of Nursing, Qingdao University, Qingdao, Shandong 266003, P.R. China, Medicine Research Center, Weifang Medical University, Weifang, Shandong 261053, P.R. China, ICU, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2621-2630
    |
    Published online on: July 16, 2018
       https://doi.org/10.3892/mmr.2018.9294
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Abstract

In our previous study, advanced glycosylation end‑product specific receptor (RAGE) was observed to bind to S100A8/A9 and cause epithelial mesenchymal transition (EMT). The results from target gene prediction revealed that microRNA (miR)‑185‑5p had a RAGE binding site. However, the function of miR‑185‑5p in the invasion and migration of breast cancer remains ambiguous. In the present study, the expression of miR‑185‑5p was examined in breast cancer tissues and cells. Clinical features revealed a negative correlation between miR‑185‑5p and tumor size, as well as in tumor differentiation and lymph node metastasis in breast cancer. In addition, miR‑185‑5p was negatively associated with RAGE, and this miRNA reversed the EMT of breast cancer by modulating RAGE in vitro. In addition, miR‑185‑5p inhibited the S100A8/A9‑induced EMT of breast cancer cells by the nuclear factor‑κB/Snail signaling pathway. Notably, miR‑185‑5p upregulation inhibited the F‑actin polymerization induced by S100A8/A9 in breast cancer. Furthermore, overexpression of miR‑185‑5p and reduction of RAGE inhibited lung metastasis node in vivo. Thus, miR‑185‑5p represents a potential therapeutic target in breast cancer by modulating RAGE.
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Copy and paste a formatted citation
Spandidos Publications style
Yin C, Zhang G, Sun R, Pan X, Wang X, Li H and Sun Y: miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE. Mol Med Rep 18: 2621-2630, 2018.
APA
Yin, C., Zhang, G., Sun, R., Pan, X., Wang, X., Li, H., & Sun, Y. (2018). miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE. Molecular Medicine Reports, 18, 2621-2630. https://doi.org/10.3892/mmr.2018.9294
MLA
Yin, C., Zhang, G., Sun, R., Pan, X., Wang, X., Li, H., Sun, Y."miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE". Molecular Medicine Reports 18.3 (2018): 2621-2630.
Chicago
Yin, C., Zhang, G., Sun, R., Pan, X., Wang, X., Li, H., Sun, Y."miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE". Molecular Medicine Reports 18, no. 3 (2018): 2621-2630. https://doi.org/10.3892/mmr.2018.9294
Copy and paste a formatted citation
x
Spandidos Publications style
Yin C, Zhang G, Sun R, Pan X, Wang X, Li H and Sun Y: miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE. Mol Med Rep 18: 2621-2630, 2018.
APA
Yin, C., Zhang, G., Sun, R., Pan, X., Wang, X., Li, H., & Sun, Y. (2018). miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE. Molecular Medicine Reports, 18, 2621-2630. https://doi.org/10.3892/mmr.2018.9294
MLA
Yin, C., Zhang, G., Sun, R., Pan, X., Wang, X., Li, H., Sun, Y."miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE". Molecular Medicine Reports 18.3 (2018): 2621-2630.
Chicago
Yin, C., Zhang, G., Sun, R., Pan, X., Wang, X., Li, H., Sun, Y."miR‑185‑5p inhibits F‑actin polymerization and reverses epithelial mesenchymal transition of human breast cancer cells by modulating RAGE". Molecular Medicine Reports 18, no. 3 (2018): 2621-2630. https://doi.org/10.3892/mmr.2018.9294
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