Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
September-2018 Volume 18 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2018 Volume 18 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study

Corrigendum in: /10.3892/mmr.2021.12444
  • Authors:
    • Zhikang Miao
    • Zhenhui Lu
    • Shixing Luo
    • Danqing Lei
    • Yi He
    • Huayu Wu
    • Jinmin Zhao
    • Li Zheng
  • View Affiliations / Copyright

    Affiliations: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Orthopedics, Ninth Affiliated Hospital of Guangxi Medical University, Beihai 536000, P.R. China, Life Sciences Institute, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Cell Biology and Genetics, School of Premedical Sciences, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
    Copyright: © Miao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3341-3349
    |
    Published online on: July 23, 2018
       https://doi.org/10.3892/mmr.2018.9307
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Mesenchymal stem cell (MSC)‑based therapy has been commonly used in cartilage reconstruction, due to its self‑renewing ability and multi‑differentiation potential. Nerve growth factor (NGF) from cobra venom has been reported to regulate chondrogenesis of bone‑derived MSCs (BMSCs) and chondrocyte metabolism. Therefore, the present study aimed to determine whether other sources of NGF behave in the same manner as NGF from natural venom. The present study compared the effects of NGF from two sources, the commercially purchased recombinant murine β‑NGF (mNGF) and cobra venom‑derived NGF (cvNGF), on chondrogenesis of BMSCs by performing hematoxylin and eosin and fluorescein diacetate/propidium iodide staining, DNA and glycosaminoglycan quantization and reverse transcription‑quantitative polymerase chain reaction to investigate cell morphology, viability, proliferation, glycosaminoglycan synthesis and cartilage‑specific gene expression. The results demonstrated that cvNGF significantly accelerated cell proliferation and upregulated the expression of cartilage‑specific genes, including aggrecan, SRY‑box 9 and collagen type II α1 chain. Conversely, cvNGF reduced the expression levels of collagen type I α1 chain (a fibrocartilage marker), runt‑related transcription factor 2 and enolase 2 compared with in the mNGF and control groups. In addition, Chinese cobra venom, which is the main resource of cvNGF, is abundant and inexpensive, thus greatly decreasing the cost. In conclusion, the present study demonstrated that cvNGF may be considered a potential growth factor for inducing chondrogenic differentiation of BMSCs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Risbud MV and Sittinger M: Tissue engineering: Advances in in vitro cartilage generation. Trends Biotechnol. 20:351–356. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Wu YN, Yang Z, Hui JH, Ouyang HW and Lee EH: Cartilaginous ECM component-modification of the micro-bead culture system for chondrogenic differentiation of mesenchymal stem cells. Biomaterials. 28:4056–4067. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Nakasa T and Ochi M: Cell based therapy for articular cartilage injury. Clin Calcium. 21:890–895. 2011.(In Japanese). PubMed/NCBI

4 

Caldwell KL and Wang J: Cell-based articular cartilage repair: The link between development and regeneration. Osteoarthritis Cartilage. 23:351–362. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Jiang T, Xu G, Wang Q, Yang L, Zheng L, Zhao J and Zhang X: In vitro expansion impaired the stemness of early passage mesenchymal stem cells for treatment of cartilage defects. Cell Death Dis. 8:e28512017. View Article : Google Scholar : PubMed/NCBI

6 

Szychlinska MA, Stoddart MJ, D'Amora U, Ambrosio L, Alini M and Musumeci G: Mesenchymal stem cell-based cartilage regeneration approach and cell senescence: Can we manipulate cell aging and function? Tissue Eng Part B Rev. 23:529–539. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Raghunath J, Salacinski HJ, Sales KM, Butler PE and Seifalian AM: Advancing cartilage tissue engineering: The application of stem cell technology. Curr Opin Biotechnol. 16:503–509. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Lu Z, Lei D, Jiang T, Yang L, Zheng L and Zhao J: Nerve growth factor from Chinese cobra venom stimulates chondrogenic differentiation of mesenchymal stem cells. Cell Death Dis. 8:e28012017. View Article : Google Scholar : PubMed/NCBI

9 

van Beuningen HM, Glansbeek HL, van der Kraan PM and van den Berg WB: Differential effects of local application of BMP-2 or TGF-beta 1 on both articular cartilage composition and osteophyte formation. Osteoarthritis Cartilage. 6:306–317. 1998. View Article : Google Scholar : PubMed/NCBI

10 

Bakker AC, van de Loo FA, van Beuningen HM, Sime P, van Lent PL, van der Kraan PM, Richards CD and van den Berg WB: Overexpression of active TGF-beta-1 in the murine knee joint: Evidence for synovial-layer-dependent chondro-osteophyte formation. Osteoarthritis Cartilage. 9:128–136. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Davidson Blaney EN, Vitters EL, van Beuningen HM, van de Loo FA, van den Berg WB and van der Kraan PM: Resemblance of osteophytes in experimental osteoarthritis to transforming growth factor beta-induced osteophytes: Limited role of bone morphogenetic protein in early osteoarthritic osteophyte formation. Arthritis Rheum. 56:4065–4073. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Li C, Jiang J, Zheng Z, Lee KS, Zhou Y, Chen E, Culiat CT, Qiao Y, Chen X, Ting K, et al: Neural EGFL-like 1 is a downstream regulator of runt-related transcription factor 2 in chondrogenic differentiation and maturation. Am J Pathol. 187:963–972. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Grässel SG: The role of peripheral nerve fibers and their neurotransmitters in cartilage and bone physiology and pathophysiology. Arthritis Res Ther. 16:4852014. View Article : Google Scholar : PubMed/NCBI

14 

Nojiri Y, Takeda S, Enomoto M, Nishitsuji H, Masuda T, Sotome S and Shinomiya K: Co-overexpression of GDNF and GFRalpha1 induces neural differentiation in neural progenitor cells in comparison to bone marrow stromal cells. J Med Dent Sci. 55:121–128. 2008.PubMed/NCBI

15 

Lipps BV: Detection of nerve growth factor (NGF) in venoms from diverse source: Isolation and characterization of NGF from the venom of honey bee (Apis melifera). J Nat Toxins. 9:13–19. 2000.PubMed/NCBI

16 

Lipps BV: Isolation of nerve growth factor (NGF) from human body fluids; saliva, serum and urine: Comparison between cobra venom and cobra serum NGF. J Nat Toxins. 9:349–356. 2000.PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Caplan AI: Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol. 213:341–347. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Tew SR, Li Y, Pothacharoen P, Tweats LM, Hawkins RE and Hardingham TE: Retroviral transduction with SOX9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes. Osteoarthritis Cartilage. 13:80–89. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Uebersax L, Merkle HP and Meinel L: Insulin-like growth factor I releasing silk fibroin scaffolds induce chondrogenic differentiation of human mesenchymal stem cells. J Control Release. 127:12–21. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Marshall OJ and Harley VR: Molecular mechanisms of SOX9 action. Mol Genet Metab. 71:455–462. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Tew SR and Clegg PD: Analysis of post transcriptional regulation of SOX9 mRNA during in vitro chondrogenesis. Tissue Eng Part A. 17:1801–1807. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Robinson D, Ash H, Yayon A, Nevo Z and Aviezer D: Characteristics of cartilage biopsies used for autologous chondrocytes transplantation. Cell Transplant. 10:203–208. 2001.PubMed/NCBI

24 

Zhang W, Chen J, Tao J, Jiang Y, Hu C, Huang L, Ji J and Ouyang HW: The use of type 1 collagen scaffold containing stromal cell-derived factor-1 to create a matrix environment conducive to partial-thickness cartilage defects repair. Biomaterials. 34:713–723. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Liu CF, Samsa WE, Zhou G and Lefebvre V: Transcriptional control of chondrocyte specification and differentiation. Semin Cell Dev Biol. 62:34–49. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Yoshii SR, Kuma A and Mizushima N: Transgenic rescue of Atg5-null mice from neonatal lethality with neuron-specific expression of ATG5: Systemic analysis of adult Atg5-deficient mice. Autophagy. 13:763–764. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Miao Z, Lu Z, Luo S, Lei D, He Y, Wu H, Zhao J and Zheng L: Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study Corrigendum in /10.3892/mmr.2021.12444. Mol Med Rep 18: 3341-3349, 2018.
APA
Miao, Z., Lu, Z., Luo, S., Lei, D., He, Y., Wu, H. ... Zheng, L. (2018). Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study Corrigendum in /10.3892/mmr.2021.12444. Molecular Medicine Reports, 18, 3341-3349. https://doi.org/10.3892/mmr.2018.9307
MLA
Miao, Z., Lu, Z., Luo, S., Lei, D., He, Y., Wu, H., Zhao, J., Zheng, L."Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study Corrigendum in /10.3892/mmr.2021.12444". Molecular Medicine Reports 18.3 (2018): 3341-3349.
Chicago
Miao, Z., Lu, Z., Luo, S., Lei, D., He, Y., Wu, H., Zhao, J., Zheng, L."Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study Corrigendum in /10.3892/mmr.2021.12444". Molecular Medicine Reports 18, no. 3 (2018): 3341-3349. https://doi.org/10.3892/mmr.2018.9307
Copy and paste a formatted citation
x
Spandidos Publications style
Miao Z, Lu Z, Luo S, Lei D, He Y, Wu H, Zhao J and Zheng L: Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study Corrigendum in /10.3892/mmr.2021.12444. Mol Med Rep 18: 3341-3349, 2018.
APA
Miao, Z., Lu, Z., Luo, S., Lei, D., He, Y., Wu, H. ... Zheng, L. (2018). Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study Corrigendum in /10.3892/mmr.2021.12444. Molecular Medicine Reports, 18, 3341-3349. https://doi.org/10.3892/mmr.2018.9307
MLA
Miao, Z., Lu, Z., Luo, S., Lei, D., He, Y., Wu, H., Zhao, J., Zheng, L."Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study Corrigendum in /10.3892/mmr.2021.12444". Molecular Medicine Reports 18.3 (2018): 3341-3349.
Chicago
Miao, Z., Lu, Z., Luo, S., Lei, D., He, Y., Wu, H., Zhao, J., Zheng, L."Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study Corrigendum in /10.3892/mmr.2021.12444". Molecular Medicine Reports 18, no. 3 (2018): 3341-3349. https://doi.org/10.3892/mmr.2018.9307
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team