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September-2018 Volume 18 Issue 3

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Article

shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth

  • Authors:
    • Juan Liu
    • Jing Sun
    • Qian Zhang
    • Zhaochong Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Radiotherapy, Zhongshan Hospital, Fudan University, Xuhui, Shanghai 200032, P.R. China
  • Pages: 3490-3496
    |
    Published online on: July 25, 2018
       https://doi.org/10.3892/mmr.2018.9317
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Abstract

Breast cancer is a heterogeneous disease with a high degree of diversity with regards to tumor histological stage and molecular subtypes. These heterogeneous characteristics determine the risk of disease progression and therapeutic resistance. Understanding tumor heterogeneity is of primary concern to identify and develop novel and specific potential targets for diagnosis and therapy. The present study analyzed 106 paired breast cancer tissues from The Cancer Genome Atlas and demonstrated that excision repair cross‑complementation group 6 like (ERCC6L), a newly discovered DNA helicase, was overexpressed in 91.51% (97/106), unchanged in 7.54% (8/106) and decreased in 0.94% (1/106) of breast cancer samples. A short hairpin RNA ERCC6L lentivirus was constructed to investigate the role of ERCC6LR in cancer. First, a Celigo Image Cytometry system was used to detect MDA‑MB‑231 cell growth number following transfection with shERCC6L‑lentivirus or NC‑lentivirus and it was identified that the growth number of fluorescent MDA‑MB‑231 cells post‑transduction with shERCC6L‑lentivirus was decreased compared with the cells transduced with NC‑lentivirus. Then, the effect of knockdown of ERCC6L expression on the cell cycle distribution and apoptosis was to analyzed using fluorescence‑activated cell sorting (FACS). The FACS data demonstrated that knockdown of ERCC6L expression levels in MDA‑MB‑231 cells significantly increased S phase population but decreased the G1 and G2/M phase populations compared with the NC group. The apoptosis rate of MDA‑MB‑231 cells post‑transduction with shERCC6L‑lentivirus for 5 days was increased to 12.16±0.146% compared with the negative control rate (4.86±0.204%). These functional studies demonstrated that knockdown of ERCC6L expression levels in MDA‑MB‑231 cells significantly inhibited breast cancer cell proliferation, disturbed cell cycle distribution and induced apoptosis in vitro. These findings suggested that ERCC6L, which is highly expressed in breast cancer, acts as an oncogene, is involved in breast cancer development and may serve as a novel molecular target for the treatment of breast cancer.
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1 

Torre LA, Siegel RL, Ward EM and Jemal A: Global cancer incidence and mortality rates and trends-an update. Cancer Epidemiol Biomarkers Prev. 25:16–27. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Johann DJ, Rodriguez-Canales J, Mukherjee S, Prieto DA, Hanson JC, Emmert-Buck M and Blonder J: Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry. J Proteome Res. 8:2310–2318. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Polyak K: Heterogeneity in breast cancer. J Clin Invest. 121:3786–3788. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Park SY, Gönen M, Kim HJ, Michor F and Polyak K: Cellular and genetic diversity in the progression of in situ human breast carcinomas to an invasive phenotype. J Clin Invest. 120:636–644. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Beca F and Polyak K: Intratumor heterogeneity in breast cancer. Adv Exp Med Biol. 882:169–189. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Karlsson E, Appelgren J, Solterbeck A, Bergenheim M, Alvariza V and Bergh J: Breast cancer during follow-up and progression - A population based cohort on new cancers and changed biology. Eur J Cancer. 50:2916–2924. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Badve S and Gökmen-Polar Y: Tumor heterogeneity in breast cancer. Adv Anat Pathol. 22:294–302. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Bai X, Zhang E, Ye H, Nandakumar V, Wang Z, Chen L, Tang C, Li J, Li H, Zhang W, et al: PIK3CA and TP53 gene mutations in human breast cancer tumors frequently detected by ion torrent DNA sequencing. PLoS One. 9:e993062014. View Article : Google Scholar : PubMed/NCBI

9 

Van Keymeulen A, Lee MY, Ousset M, Brohée S, Rorive S, Giraddi RR, Wuidart A, Bouvencourt G, Dubois C, Salmon I, et al: Reactivation of multipotency by oncogenic PIK3CA induces breast tumour heterogeneity. Nature. 525:119–123. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Koren S, Reavie L, Couto JP, De Silva D, Stadler MB, Roloff T, Britschgi A, Eichlisberger T, Kohler H, Aina O, et al: PIK3CA(H1047R) induces multipotency and multi-lineage mammary tumours. Nature. 525:114–118. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

12 

King KL and Cidlowski JA: Cell cycle regulation and apoptosis. Annu Rev Physiol. 60:601–617. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Alenzi FQ: Links between apoptosis, proliferation and the cell cycle. Br J Biomed Sci. 61:99–102. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Foote FW Jr and Stewart FW: A histologic classification of carcinoma of the breast. Surgery. 19:74–99. 1946.PubMed/NCBI

15 

McGranahan N and Swanton C: Biological and therapeutic impact of intratumor heterogeneity in cancer evolution. Cancer Cell. 27:15–26. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Pu SY, Yu Q, Wu H, Jiang JJ, Chen XQ, He YH and Kong QP: ERCC6L, a DNA helicase, is involved in cell proliferation and associated with survival and progress in breast and kidney cancers. Oncotarget. 8:42116–42124. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Baumann C, Körner R, Hofmann K and Nigg EA: PICH, a centromere-associated SNF2 family ATPase, is regulated by Plk1 and required for the spindle checkpoint. Cell. 128:101–114. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Lin Z, Zhang X, Tuo J, Guo Y, Green B, Chan CC, Tan W, Huang Y, Ling W, Kadlubar FF, et al: A variant of the Cockayne syndrome B gene ERCC6 confers risk of lung cancer. Hum Mutat. 29:113–122. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Abbasi R, Ramroth H, Becher H, Dietz A, Schmezer P and Popanda O: Laryngeal cancer risk associated with smoking and alcohol consumption is modified by genetic polymorphisms in ERCC5, ERCC6 and RAD23B but not by polymorphisms in five other nucleotide excision repair genes. Int J Cancer. 125:1431–1439. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Liu JW, He CY, Sun LP, Xu Q, Xing CZ and Yuan Y: The DNA repair gene ERCC6 rs1917799 polymorphism is associated with gastric cancer risk in Chinese. Asian Pac J Cancer Prev. 14:6103–6108. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Chang CH, Chiu CF, Wang HC, Wu HC, Tsai RY, Tsai CW, Wang RF, Wang CH, Tsou YA and Bau DT: Significant association of ERCC6 single nucleotide polymorphisms with bladder cancer susceptibility in Taiwan. Anticancer Res. 29:5121–5124. 2009.PubMed/NCBI

22 

Ma H, Hu Z, Wang H, Jin G, Wang Y, Sun W, Chen D, Tian T, Jin L, Wei Q, et al: ERCC6/CSB gene polymorphisms and lung cancer risk. Cancer Lett. 273:172–176. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Ramaniuk VP, Nikitchenko NV, Savina NV, Kuzhir TD, Rolevich AI, Krasny SA, Sushinsky VE and Goncharova RI: Polymorphism of DNA repair genes OGG1, XRCC1, XPD and ERCC6 in bladder cancer in Belarus. Biomarkers. 19:509–516. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Yin Y, Tang L, Zhang J, Tang B and Li Z: Molecular cloning and gene expression analysis of Ercc6l in Sika Deer (Cervus nippon hortulorum). PLoS One. 6:e209292011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Sun J, Zhang Q and Zeng Z: shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth. Mol Med Rep 18: 3490-3496, 2018.
APA
Liu, J., Sun, J., Zhang, Q., & Zeng, Z. (2018). shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth. Molecular Medicine Reports, 18, 3490-3496. https://doi.org/10.3892/mmr.2018.9317
MLA
Liu, J., Sun, J., Zhang, Q., Zeng, Z."shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth". Molecular Medicine Reports 18.3 (2018): 3490-3496.
Chicago
Liu, J., Sun, J., Zhang, Q., Zeng, Z."shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth". Molecular Medicine Reports 18, no. 3 (2018): 3490-3496. https://doi.org/10.3892/mmr.2018.9317
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Sun J, Zhang Q and Zeng Z: shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth. Mol Med Rep 18: 3490-3496, 2018.
APA
Liu, J., Sun, J., Zhang, Q., & Zeng, Z. (2018). shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth. Molecular Medicine Reports, 18, 3490-3496. https://doi.org/10.3892/mmr.2018.9317
MLA
Liu, J., Sun, J., Zhang, Q., Zeng, Z."shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth". Molecular Medicine Reports 18.3 (2018): 3490-3496.
Chicago
Liu, J., Sun, J., Zhang, Q., Zeng, Z."shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth". Molecular Medicine Reports 18, no. 3 (2018): 3490-3496. https://doi.org/10.3892/mmr.2018.9317
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