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September-2018 Volume 18 Issue 3

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Article

Knockdown of miR‑935 increases paclitaxel sensitivity via regulation of SOX7 in non‑small‑cell lung cancer

  • Authors:
    • Bing Peng
    • Chao Li
    • Peng Cai
    • Liuyang Yu
    • Bolin Zhao
    • Guiming Chen
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The Second People's Hospital of Jingmen, Jingmen, Hubei 448000, P.R. China
  • Pages: 3397-3402
    |
    Published online on: July 27, 2018
       https://doi.org/10.3892/mmr.2018.9330
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Abstract

Sex determining region Y‑box (SOX)7 is a member of the SOX family and is responsible for various developmental processes. As a tumor suppressor, decreased expression of SOX7 has been observed in several cancer types, including non‑small‑cell lung cancer (NSCLC). However, the mechanism underlying SOX7 downregulation and its role in chemoresistance in NSCLC remains poorly understood. In the present study, the inhibition of microRNA (miR)‑935 increased the expression of SOX7 at the mRNA and protein levels in A549 cells. The luciferase reporter assay verified that miR‑935 could directly bind to the 3'untranslated region of SOX7 mRNA to suppress its expression in A549 cells. In addition, the inhibition of miR‑935 enhanced the anticancer effect of paclitaxel, i.e., induced cell growth arrest and apoptosis in A549 cells. It was further observed that the inhibition of miR‑935 decreased the B cell lymphoma (Bcl)‑2 and phosphorylated‑RAC‑α serine/threonine‑protein kinase (AKT) protein levels and increased the Bcl‑2 associated X, apoptosis regulator protein levels, without affecting the AKT levels in the presence of paclitaxel within A549 cells. The findings of the present study validate miR‑935 as a predictor of paclitaxel sensitivity in NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Peng B, Li C, Cai P, Yu L, Zhao B and Chen G: Knockdown of miR‑935 increases paclitaxel sensitivity via regulation of SOX7 in non‑small‑cell lung cancer. Mol Med Rep 18: 3397-3402, 2018.
APA
Peng, B., Li, C., Cai, P., Yu, L., Zhao, B., & Chen, G. (2018). Knockdown of miR‑935 increases paclitaxel sensitivity via regulation of SOX7 in non‑small‑cell lung cancer. Molecular Medicine Reports, 18, 3397-3402. https://doi.org/10.3892/mmr.2018.9330
MLA
Peng, B., Li, C., Cai, P., Yu, L., Zhao, B., Chen, G."Knockdown of miR‑935 increases paclitaxel sensitivity via regulation of SOX7 in non‑small‑cell lung cancer". Molecular Medicine Reports 18.3 (2018): 3397-3402.
Chicago
Peng, B., Li, C., Cai, P., Yu, L., Zhao, B., Chen, G."Knockdown of miR‑935 increases paclitaxel sensitivity via regulation of SOX7 in non‑small‑cell lung cancer". Molecular Medicine Reports 18, no. 3 (2018): 3397-3402. https://doi.org/10.3892/mmr.2018.9330
Copy and paste a formatted citation
x
Spandidos Publications style
Peng B, Li C, Cai P, Yu L, Zhao B and Chen G: Knockdown of miR‑935 increases paclitaxel sensitivity via regulation of SOX7 in non‑small‑cell lung cancer. Mol Med Rep 18: 3397-3402, 2018.
APA
Peng, B., Li, C., Cai, P., Yu, L., Zhao, B., & Chen, G. (2018). Knockdown of miR‑935 increases paclitaxel sensitivity via regulation of SOX7 in non‑small‑cell lung cancer. Molecular Medicine Reports, 18, 3397-3402. https://doi.org/10.3892/mmr.2018.9330
MLA
Peng, B., Li, C., Cai, P., Yu, L., Zhao, B., Chen, G."Knockdown of miR‑935 increases paclitaxel sensitivity via regulation of SOX7 in non‑small‑cell lung cancer". Molecular Medicine Reports 18.3 (2018): 3397-3402.
Chicago
Peng, B., Li, C., Cai, P., Yu, L., Zhao, B., Chen, G."Knockdown of miR‑935 increases paclitaxel sensitivity via regulation of SOX7 in non‑small‑cell lung cancer". Molecular Medicine Reports 18, no. 3 (2018): 3397-3402. https://doi.org/10.3892/mmr.2018.9330
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