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Article Open Access

Knockdown of long noncoding RNA GHET1 inhibits cell‑cycle progression and invasion of gastric cancer cells

  • Authors:
    • Ying Xia
    • Zhiqiang Yan
    • Ying Wan
    • Sixi Wei
    • Ying Bi
    • Juanjuan Zhao
    • Juanjuan Liu
    • Dezhong Joshua Liao
    • Hai Huang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Biochemistry, School of Clinical Laboratory Science, Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of Gastrointestinal Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of Pathology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
    Copyright: © Xia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3375-3381
    |
    Published online on: July 27, 2018
       https://doi.org/10.3892/mmr.2018.9332
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Abstract

GHET1 is an oncogenic long noncoding RNA (lncRNA) that promotes the proliferation and invasion of many malignant cell types. However, the function and underlying mechanisms of lncRNA GHET1 in gastric cancer are not fully understood. In this study, the expression of GHET1 was investigated in gastric cancer and it was determined whether GHET1 may potentially be used as a biomarker for the disease. The gastric cancer cell lines MGC‑803 and AGS were transfected with GHET1‑directed small interfering RNA (siRNA) and the changes in phenotype and cell‑cycle‑related molecules were assessed. The downregulation of GHET1 induced G0/G1‑phase arrest in gastric cancer cells and inhibited their proliferation, migration, and invasion. DNA synthesis and the expression of proliferating cell nuclear antigen (PCNA) decreased, which was consistent with the results of the CCK‑8 assay. The levels of specific cell‑cycle regulators were determined and the expression and activities of positive cell‑cycle regulators (cyclin D, CDK4, CDK6, cyclin E, CDK2) were reduced, whereas those of a negative regulator (P21) were increased in GHET1‑knockdown cells. Taken together, the present findings show that the downregulation of GHET1 not only inhibits the migration and invasion of gastric cancer cells, but also inhibits their proliferation, at least in part by upregulating P21 expression and downregulating cyclin and CDK expression to inhibit the G0/G1 to S phase transition. The present findings may provide a potential therapeutic target for gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xia Y, Yan Z, Wan Y, Wei S, Bi Y, Zhao J, Liu J, Liao DJ and Huang H: Knockdown of long noncoding RNA GHET1 inhibits cell‑cycle progression and invasion of gastric cancer cells. Mol Med Rep 18: 3375-3381, 2018.
APA
Xia, Y., Yan, Z., Wan, Y., Wei, S., Bi, Y., Zhao, J. ... Huang, H. (2018). Knockdown of long noncoding RNA GHET1 inhibits cell‑cycle progression and invasion of gastric cancer cells. Molecular Medicine Reports, 18, 3375-3381. https://doi.org/10.3892/mmr.2018.9332
MLA
Xia, Y., Yan, Z., Wan, Y., Wei, S., Bi, Y., Zhao, J., Liu, J., Liao, D. J., Huang, H."Knockdown of long noncoding RNA GHET1 inhibits cell‑cycle progression and invasion of gastric cancer cells". Molecular Medicine Reports 18.3 (2018): 3375-3381.
Chicago
Xia, Y., Yan, Z., Wan, Y., Wei, S., Bi, Y., Zhao, J., Liu, J., Liao, D. J., Huang, H."Knockdown of long noncoding RNA GHET1 inhibits cell‑cycle progression and invasion of gastric cancer cells". Molecular Medicine Reports 18, no. 3 (2018): 3375-3381. https://doi.org/10.3892/mmr.2018.9332
Copy and paste a formatted citation
x
Spandidos Publications style
Xia Y, Yan Z, Wan Y, Wei S, Bi Y, Zhao J, Liu J, Liao DJ and Huang H: Knockdown of long noncoding RNA GHET1 inhibits cell‑cycle progression and invasion of gastric cancer cells. Mol Med Rep 18: 3375-3381, 2018.
APA
Xia, Y., Yan, Z., Wan, Y., Wei, S., Bi, Y., Zhao, J. ... Huang, H. (2018). Knockdown of long noncoding RNA GHET1 inhibits cell‑cycle progression and invasion of gastric cancer cells. Molecular Medicine Reports, 18, 3375-3381. https://doi.org/10.3892/mmr.2018.9332
MLA
Xia, Y., Yan, Z., Wan, Y., Wei, S., Bi, Y., Zhao, J., Liu, J., Liao, D. J., Huang, H."Knockdown of long noncoding RNA GHET1 inhibits cell‑cycle progression and invasion of gastric cancer cells". Molecular Medicine Reports 18.3 (2018): 3375-3381.
Chicago
Xia, Y., Yan, Z., Wan, Y., Wei, S., Bi, Y., Zhao, J., Liu, J., Liao, D. J., Huang, H."Knockdown of long noncoding RNA GHET1 inhibits cell‑cycle progression and invasion of gastric cancer cells". Molecular Medicine Reports 18, no. 3 (2018): 3375-3381. https://doi.org/10.3892/mmr.2018.9332
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