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Article Open Access

In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis

  • Authors:
    • Jiarong Gao
    • Liangbing Wei
    • Junmei Song
    • Hui Jiang
    • Yachen Gao
    • Xi Wu
    • Shuangzhi Xu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui 230031, P.R. China, College of Chemistry and Material Engineering, Chaohu University, Hefei, Anhui 230031, P.R. China , Department of Urology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui 230031, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3683-3690
    |
    Published online on: August 3, 2018
       https://doi.org/10.3892/mmr.2018.9355
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Abstract

Chronic glomerulonephritis (CGN) is the most common form of glomerular disease; however, its associated molecular mechanisms remain unclear. Spleen tyrosine kinase (Syk) is a key mediator of B‑receptor signaling on the surface of inflammatory cells. The primary target for R406 is Syk. The aim of the present study was to investigate the molecular mechanisms involved in a rat model of CGN induced by adriamycin (ADR) and in the rat glomerular mesangial cell line, HBZY‑1, stimulated by lipopolysaccharide (LPS). CGN was induced in the rat models by two intravenous injections of ADR into the tail: 3.5 mg/kg ADR was given on the first day and 3.0 mg/kg on the fourteenth day. HBZY‑1 cells were incubated with 0.5 µg/ml LPS for 48 h. The pathological alterations in the kidney tissues were observed by hematoxylin and eosin staining. The 24 h urinary protein, blood urea nitrogen (BUN) and creatinine levels were measured using an automatic biochemistry analyzer. The mRNA expression levels of Syk, Ras, mitogen activated protein kinase kinase (MEK), extracellular signal regulated kinase (ERK)1/2 and c‑Fos was measured by reverse transcription‑quantitative polymerase chain reaction. Subsequently, the protein levels of phosphorylated (p)‑Syk, Ras, p‑MEK1/2, p‑ERK1/2 and c‑Fos were measured by western blot analysis. In the model group, 24 h urinary protein, BUN and creatinine levels were increased when compared with the normal group (P<0.05). In addition, compared with the normal group, the mRNA and protein levels of the Syk/Ras/c‑Fos pathway components in vitro and in vivo were markedly increased, inhibiting the abnormal cell viability of mesangial cells. In conclusion, the results of the present study suggested a potential role for the Syk/Ras/c‑Fos signaling pathway in CGN, which indicated the necessity for further investigation at the clinical level.
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View References

1 

Floege J and Amann K: Primary glomerulonephritides. Lancet. 387:2036–2048. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Zhang H, Ying Y, Chen Y, Lu X and Huang Y: Effect of chronic glomerulonephritis on the semen quality and cytokines in the semen of infertile males. Am J Reprod Immunol. 77:2017.https://doi.org/10.1111/aji.12598simple10.1111/aji.12598 View Article : Google Scholar

3 

Chebotareva NV, Bobkova IN, Neprintseva NV, Kozlovskaia LV and Malkandueva ZT: Urinary biomarkers for podocyte injury: Significance for evaluating the course and prognosis of chronic glomerulonephritis. Ter Arkh. 87:34–39. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Satirapoj B, Jirawatsiwaporn K, Tangwonglert T and Choovichian P: Performance of the estimated glomerular filtration rate creatinine and cystatin C based equations in Thai patients with chronic glomerulonephritis. Int J Nephrol Renovasc Dis. 8:145–150. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Dudnyk V, Zvenigorodska A and Guminska G: Genetic aspects of chronic glomerulonephritis. Lik Sprava. 159–160. 2015.(In Ukrainian). PubMed/NCBI

6 

Hule GP, Karmarkar MG, Cameron A, Hase N, Khopkar U, Mehta PR, McNeilly CL, McMillan D and Sriprakash KS: Seropositivity for antibodies to DRS-G, a virulence factor from streptococcus dysgalactiae subsp. equisimilis, is an independent risk factor for poststreptococcus glomerulonephritis and chronic kidney disease in Mumbai, India. Clin Vaccine Immunol. 22:938–942. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Gao JR, Qin XJ, Jiang H, Wang T, Song JM and Xu SZ: Screening and functional analysis of differentially expressed genes in chronic glomerulonephritis by whole genome microarray. Gene. 589:72–80. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Campa MJ, Moody MA, Zhang R, Liao HX, Gottlin EB and Patz EF Jr: Interrogation of individual intratumoral B lymphocytes from lung cancer patients for molecular target discovery. Cancer Immunol Immunother. 65:171–180. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Zeng KW, Wang S, Dong X, Jiang Y, Jin HW and Tu PF: Sesquiterpene dimmer (DSF-27) inhibits the release of neuroinflammatory mediators from microglia by targeting spleen tyrosine kinase (Syk) and Janus kinase 2 (Jak2): Two major non-receptor tyrosine signaling proteins involved in inflammatory events. Toxicol Appl Pharmacol. 275:244–256. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Wu L, Zhang L, Zhao J, Ning X, Mu C and Wang C: Cloning and expression of a transcription factor activator protein-1 (AP-1) member identified from manila clam Venerupis philippinarum. Gene. 557:106–111. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Lee DS, Yang SH, Kim HL, Joo KW, Lim CS, Chae DW, Kim S, Lee JS and Kim YS: Recombinant uteroglobin prevents the experimental crescentic glomerulonephritis. Kidney Int. 66:1061–1067. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Gohda T, Makita Y, Shike T, Funabiki K, Shirato I and Tomino Y: Dilazep hydrochloride, an antiplatelet drug, inhibits lipopolysaccharide-induced mouse mesangial cell IL-6 secretion and proliferation. Kidney Blood Press Res. 24:33–38. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Zhao H, Liu Z, Shen H, Jin S and Zhang S: Glycyrrhizic acid pretreatment prevents sepsis-induced acute kidney injury via suppressing inflammation, apoptosis and oxidative stress. Eur J Pharmacol. 781:92–99. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Gao JR, Qin XJ, Jiang H, Wang T, Song JM and Xu SZ: The effects of Qi Teng Xiao Zhuo granules, traditional Chinese medicine, on the expression of genes in chronic glomerulonephritis rats. J Ethnopharmacol. 193:140–149. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Sun LY, Wang Y, Chen SF, Sun ML and Li XM: The effects of strict dietary salt restriction on blood pressure and proteinuria in chronic glomerulonephritis patients. Zhonghua Nei Ke Za Zhi. 48:995–998. 2009.(In Chinese). PubMed/NCBI

17 

Zhong WQ, Liu GX, Yang YM, Cai X and Huang ZL: Clinical effect of treatment with lipo-prostaglandin E1 on the patients with chronic glomerulonephritis. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 16:292–294. 2004.(In Chinese). PubMed/NCBI

18 

Wang NH: Clinical research on effects of the integrative medicine on chronic nephritis. Clin J Chin Med. 10:94–95. 2015.(In Chinese).

19 

Ding SY, Zheng PD, He LQ, Hou WG, Zou Y and Gao JD: The research on xiaochaihu decoction improving the inflammation of chronic glomerulonephritis patients and relieving the proteninuria. Zhongguo Zhong Xi Yi Jie He Za Zhi. 33:21–26. 2013.(In Chinese). PubMed/NCBI

20 

Chiu HY, Huang HL, Li CH, Yin YJ, Chen HA, Hsu ST, Lin SJ, Tsai TF and Ho SY: Increased risk of glomerulonephritis and chronic kidney disease in relation to the severity of psoriasis, concomitant medication, and comorbidity: A nationwide population-based cohort study. Br J Dermatol. 173:146–154. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Gobessi S, Laurenti L, Longo PG, Carsetti L, Berno V, Sica S, Leone G and Efremov DG: Inhibition of constitutive and BCR-induced Syk activation downregulates Mcl-1 and induces apoptosis in chronic lymphocytic leukemia B cells. Leukemia. 23:686–697. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Ying H, Li Z, Yang L and Zhang J: Syk mediates BCR- and CD40-signaling integration during B cell activation. Immunobiology. 216:566–570. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Li HL, Forman MS, Kurosaki T and Puré E: Syk is required for BCR-mediated activation of p90Rsk, but not p70S6k, via a mitogen-activated protein kinase-independent pathway in B cells. J Biol Chem. 272:18200–18208. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Vogel US, Dixon RA, Schaber MD, Diehl RE, Marshall MS, Scolnick EM, Sigal IS and Gibbs JB: Cloning of bovine GAP and its interaction with oncogenic ras p21. Nature. 335:90–93. 1988. View Article : Google Scholar : PubMed/NCBI

25 

Nishiyama A, Yao L, Nagai Y, Miyata K, Yoshizumi M, Kagami S, Kondo S, Kiyomoto H, Shokoji T, Kimura S, et al: Possible contributions of reactive oxygen species and mitogen-activated protein kinase to renal injury in aldosterone/salt-induced hypertensive rats. Hypertension. 43:841–848. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Geng W, Wei R, Liu S, Tang L, Zhu H, Chen P, Wu J, Zhang X, Zhu F, Yin Z and Chen X: Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis. Biol Res. 49:172016. View Article : Google Scholar : PubMed/NCBI

27 

Zhang MX, Li L and Wang XY: Expression of Raf kinase inhibitor protein and p-ERK in renal tissues of diabetic rats. Chin J Pathophysiol. 29:358–360. 2013.(In Chinese).

28 

Chen D, Fong HW and Davis JS: Induction of c-fos and c-jun messenger ribonucleic acid expression by prostaglandin F2alpha is mediated by a protein kinase C-dependent extracellular signal-regulated kinase mitogen-activated protein kinase pathway in bovine luteal cells. Endocrinology. 142:887–895. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Zu N, Li P, Li N, Choy P and Gong Y: Mechanism of saikosaponin-d in the regulation of rat mesangial cell proliferation and synthesis of extracellular matrix proteins. Biochem Cell Biol. 85:169–174. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Chen D, Li Y, Mei Y, Geng W, Yang J, Hong Q, Feng Z, Cai G, Zhu H, Shi S, et al: miR-34a regulates mesangial cell proliferation via the PDGFR-β/Ras-MAPK signaling pathway. Cell Mol Life Sci. 71:4027–4042. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gao J, Wei L, Song J, Jiang H, Gao Y, Wu X and Xu S: In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis. Mol Med Rep 18: 3683-3690, 2018.
APA
Gao, J., Wei, L., Song, J., Jiang, H., Gao, Y., Wu, X., & Xu, S. (2018). In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis. Molecular Medicine Reports, 18, 3683-3690. https://doi.org/10.3892/mmr.2018.9355
MLA
Gao, J., Wei, L., Song, J., Jiang, H., Gao, Y., Wu, X., Xu, S."In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis". Molecular Medicine Reports 18.4 (2018): 3683-3690.
Chicago
Gao, J., Wei, L., Song, J., Jiang, H., Gao, Y., Wu, X., Xu, S."In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis". Molecular Medicine Reports 18, no. 4 (2018): 3683-3690. https://doi.org/10.3892/mmr.2018.9355
Copy and paste a formatted citation
x
Spandidos Publications style
Gao J, Wei L, Song J, Jiang H, Gao Y, Wu X and Xu S: In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis. Mol Med Rep 18: 3683-3690, 2018.
APA
Gao, J., Wei, L., Song, J., Jiang, H., Gao, Y., Wu, X., & Xu, S. (2018). In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis. Molecular Medicine Reports, 18, 3683-3690. https://doi.org/10.3892/mmr.2018.9355
MLA
Gao, J., Wei, L., Song, J., Jiang, H., Gao, Y., Wu, X., Xu, S."In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis". Molecular Medicine Reports 18.4 (2018): 3683-3690.
Chicago
Gao, J., Wei, L., Song, J., Jiang, H., Gao, Y., Wu, X., Xu, S."In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis". Molecular Medicine Reports 18, no. 4 (2018): 3683-3690. https://doi.org/10.3892/mmr.2018.9355
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