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Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway

  • Authors:
    • Zhen Wang
    • Gang Han
    • Qinghong Liu
    • Wenyuan Zhang
    • Jinshan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Guizhou People's Hospital, Guiyang, Guizhou 550002, P.R. China, Department of Urology, Chinese PLA 252 Hospital, Baoding, Hebei 071000, P.R. China, Department of Urology, East Hospital, Tongji University School of Medicine, Shanghai 200123, P.R. China, Department of Urology, East Hospital Affiliated to Ji'an Hospital, Ji'an, Jiangxi 343000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3800-3808
    |
    Published online on: August 14, 2018
       https://doi.org/10.3892/mmr.2018.9388
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Abstract

Brain‑type glycogen phosphorylase (PYGB) is an enzyme that metabolizes glycogen, whose function is to provide energy for an organism in an emergency state. The present study purposed to investigate the role and mechanism of PYGB silencing on the growth and apoptosis of prostate cancer cells. A cell counting kit‑8 assay and flow cytometry were performed to determine the cell viability, apoptosis and reactive oxygen species (ROS) content, respectively. Colorimetry was performed to analyze the activity of caspase‑3. Western blotting and reverse transcription‑quantitative polymerase chain reaction were used to evaluate the associated mRNA and protein expression levels. The results revealed that PYGB was upregulated in prostate cancer tissues and was associated with disease progression. In addition, PYGB silencing suppressed the cell viability of PC3 cells. PYGB silencing promoted apoptosis of PC3 cells via the regulation of the expression levels of cleaved‑poly (adenosine diphosphate‑ribose) polymerase, cleaved‑caspase‑3, B‑cell lymphoma‑2 (Bcl‑2) and Bcl‑2‑associated X protein. PYGB silencing increased the ROS content in PC3 cells, and affected nuclear factor (NF)‑κB/nuclear factor‑erythroid 2‑related factor 2 (Nrf2) signaling pathways in PC3 cells. In conclusion, PYGB silencing suppressed the growth and promoted the apoptosis of prostate cancer cells by affecting the NF‑κB/Nrf2 signaling pathway. The present study provided evidence that may lead to the development of a potential therapeutic strategy for prostate cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Han G, Liu Q, Zhang W and Wang J: Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway. Mol Med Rep 18: 3800-3808, 2018.
APA
Wang, Z., Han, G., Liu, Q., Zhang, W., & Wang, J. (2018). Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway. Molecular Medicine Reports, 18, 3800-3808. https://doi.org/10.3892/mmr.2018.9388
MLA
Wang, Z., Han, G., Liu, Q., Zhang, W., Wang, J."Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway". Molecular Medicine Reports 18.4 (2018): 3800-3808.
Chicago
Wang, Z., Han, G., Liu, Q., Zhang, W., Wang, J."Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway". Molecular Medicine Reports 18, no. 4 (2018): 3800-3808. https://doi.org/10.3892/mmr.2018.9388
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Han G, Liu Q, Zhang W and Wang J: Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway. Mol Med Rep 18: 3800-3808, 2018.
APA
Wang, Z., Han, G., Liu, Q., Zhang, W., & Wang, J. (2018). Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway. Molecular Medicine Reports, 18, 3800-3808. https://doi.org/10.3892/mmr.2018.9388
MLA
Wang, Z., Han, G., Liu, Q., Zhang, W., Wang, J."Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway". Molecular Medicine Reports 18.4 (2018): 3800-3808.
Chicago
Wang, Z., Han, G., Liu, Q., Zhang, W., Wang, J."Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway". Molecular Medicine Reports 18, no. 4 (2018): 3800-3808. https://doi.org/10.3892/mmr.2018.9388
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