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Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells

  • Authors:
    • Bisheng Zhu
    • Qisheng Liu
    • Qi Han
    • Bohang Zeng
    • Jingqi Chen
    • Qiuju Xiao
  • View Affiliations / Copyright

    Affiliations: Oncology Department, Xingning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, Xianning, Hubei 437000, P.R. China, Department of Gastroenterology, Xingning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, Xianning, Hubei 437000, P.R. China, Oncology Department, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3932-3940
    |
    Published online on: August 20, 2018
       https://doi.org/10.3892/mmr.2018.9401
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Abstract

Cervical cancer is one of the most common malignancies that seriously threatens women's health. Krüppel‑like factors (KLFs) have been reported to be associated with the progression of cervical cancer. The role of KLF1 in cervical cancer, which still remains unclear, was investigated in the present study. The expression of KLF1 was detected in different cervical cell lines by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting. Cell proliferation, metastasis and invasion were respectively detected by Cell Counting Kit‑8, wound healing and transwell assays. Associated factor expression was also detected by RT‑qPCR and western blotting. In addition, the phosphorylation levels of phosphatidylinositol‑3‑kinase (PI3K) and protein kinase B (Akt) were determined by western blot analysis. The results revealed that KLF1 expression was promoted in SiHa, Caski and C4‑1 cervical cancer cells. However, KLF1 knockdown suppressed cell proliferation, metastasis and invasion in SiHa cervical cancer cells. KLF1 knockdown also inhibited the expressions of Ki67, metastasis‑associated antigen 1 and matrix metalloproteinase (MMP)‑2. KLF1 knockdown promoted the expressions of nonmetastatic clone 23 type 1 and tissue inhibitor of metalloproteinase‑2, and the expression of MMP‑9 was promoted slightly as well. In addition, KLF1 knockdown inhibited the PI3K/Akt signaling pathway. Hence, it was concluded that KLF1 promoted metastasis and invasion via the PI3K/Akt signaling pathway in cervical cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu B, Liu Q, Han Q, Zeng B, Chen J and Xiao Q: Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells. Mol Med Rep 18: 3932-3940, 2018.
APA
Zhu, B., Liu, Q., Han, Q., Zeng, B., Chen, J., & Xiao, Q. (2018). Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells. Molecular Medicine Reports, 18, 3932-3940. https://doi.org/10.3892/mmr.2018.9401
MLA
Zhu, B., Liu, Q., Han, Q., Zeng, B., Chen, J., Xiao, Q."Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells". Molecular Medicine Reports 18.4 (2018): 3932-3940.
Chicago
Zhu, B., Liu, Q., Han, Q., Zeng, B., Chen, J., Xiao, Q."Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells". Molecular Medicine Reports 18, no. 4 (2018): 3932-3940. https://doi.org/10.3892/mmr.2018.9401
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu B, Liu Q, Han Q, Zeng B, Chen J and Xiao Q: Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells. Mol Med Rep 18: 3932-3940, 2018.
APA
Zhu, B., Liu, Q., Han, Q., Zeng, B., Chen, J., & Xiao, Q. (2018). Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells. Molecular Medicine Reports, 18, 3932-3940. https://doi.org/10.3892/mmr.2018.9401
MLA
Zhu, B., Liu, Q., Han, Q., Zeng, B., Chen, J., Xiao, Q."Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells". Molecular Medicine Reports 18.4 (2018): 3932-3940.
Chicago
Zhu, B., Liu, Q., Han, Q., Zeng, B., Chen, J., Xiao, Q."Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells". Molecular Medicine Reports 18, no. 4 (2018): 3932-3940. https://doi.org/10.3892/mmr.2018.9401
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