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Article Open Access

proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes

  • Authors:
    • Shen Liu
    • Wei Guo
    • Hengxing Zhou
    • Liang Tang
    • Shiqing Feng
    • Jin‑Hua Zhong
    • Xin‑Fu Zhou
  • View Affiliations / Copyright

    Affiliations: School of Pharmacy and Medical Science and Sansom Institute, Division of Health Sciences, University of South Australia, Adelaide SA5000, Australia, Orthopaedic Department of Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Orthopaedic Department of Tianjin Haihe Hospital, Tianjin 300000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3809-3817
    |
    Published online on: August 21, 2018
       https://doi.org/10.3892/mmr.2018.9407
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Abstract

In contrast with mature brain‑derived neurotrophic factor (mBDNF), proBDNF induces cell apoptosis. However, the function of proBDNF in oligodendrocytes remains unclear. In the present study, the OLN‑93 oligodendroglia cell line was utilized as an in vitro model to analyse the functions of proBDNF in oligodendroglia. p75NTR, sortilin and proBDNF were expressed in cultured OLN‑93 cells. It was indicated that proBDNF inhibited OLN‑93 cell proliferation in a dose‑dependent manner as determined using the MTT assay and BrdU staining. Furthermore, proBDNF suppressed the migration of OLN‑93 cells as demonstrated using the scratch assay. proBDNF also decreased cell viability and promoted apoptosis as indicated by activated cysteine‑aspartic acid protease‑3 (caspase‑3) immunocytochemistry. Notably, anti‑proBDNF treatment neutralized the effect of proBDNF and resulted in increased cell proliferation and migration and decreased apoptosis. However, these effects were not observed in the presence of recombinant p75NTR extracellular domain‑human FC fusion protein and p75NTR antibody, indicating that proBDNF imparts its inhibitory effects on oligodendrocytes through the p75NTR signal pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Liu S, Guo W, Zhou H, Tang L, Feng S, Zhong JH and Zhou XF: proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes. Mol Med Rep 18: 3809-3817, 2018.
APA
Liu, S., Guo, W., Zhou, H., Tang, L., Feng, S., Zhong, J., & Zhou, X. (2018). proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes. Molecular Medicine Reports, 18, 3809-3817. https://doi.org/10.3892/mmr.2018.9407
MLA
Liu, S., Guo, W., Zhou, H., Tang, L., Feng, S., Zhong, J., Zhou, X."proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes". Molecular Medicine Reports 18.4 (2018): 3809-3817.
Chicago
Liu, S., Guo, W., Zhou, H., Tang, L., Feng, S., Zhong, J., Zhou, X."proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes". Molecular Medicine Reports 18, no. 4 (2018): 3809-3817. https://doi.org/10.3892/mmr.2018.9407
Copy and paste a formatted citation
x
Spandidos Publications style
Liu S, Guo W, Zhou H, Tang L, Feng S, Zhong JH and Zhou XF: proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes. Mol Med Rep 18: 3809-3817, 2018.
APA
Liu, S., Guo, W., Zhou, H., Tang, L., Feng, S., Zhong, J., & Zhou, X. (2018). proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes. Molecular Medicine Reports, 18, 3809-3817. https://doi.org/10.3892/mmr.2018.9407
MLA
Liu, S., Guo, W., Zhou, H., Tang, L., Feng, S., Zhong, J., Zhou, X."proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes". Molecular Medicine Reports 18.4 (2018): 3809-3817.
Chicago
Liu, S., Guo, W., Zhou, H., Tang, L., Feng, S., Zhong, J., Zhou, X."proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes". Molecular Medicine Reports 18, no. 4 (2018): 3809-3817. https://doi.org/10.3892/mmr.2018.9407
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