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Article

Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix

  • Authors:
    • Lijun Shi
    • Fengli Han
    • Chenglong Shi
    • Yan Huang
    • Yan Liu
    • Xiaojia Chang
  • View Affiliations / Copyright

    Affiliations: Attogen Biomedical, Ltd., Suzhou, Jiangsu 215123, P.R. China
  • Pages: 3949-3956
    |
    Published online on: August 22, 2018
       https://doi.org/10.3892/mmr.2018.9416
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Abstract

Human papillomavirus (HPV) E7 protein expression is caused by HPV viral DNA integration into human cellular DNA, and is a prerequisite for the development and progression of cervical cancer. The present study aimed to evaluate the role of E7 protein as a biomarker for identification of transformed cervical epithelial cells during the early stages of cervical cancer. Specific monoclonal antibodies to the E7 protein of high‑risk HPVs were generated and characterized for applications in immunocytochemistry and immunohistochemistry using cervical epithelial cells or biopsy tissue slides. The specificity and feasibility for detecting precancerous cells in cervical exfoliated epithelial cells was demonstrated. In addition, antibody staining of cervix biopsies indicated the pathological grades of cervical cancer and precancerous lesions. The results of the present study demonstrated the potential benefit of using E7 protein as a novel and specific clinical diagnostic marker to distinguish transient HPV infections from malignant and pre‑malignant lesions.
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Copy and paste a formatted citation
Spandidos Publications style
Shi L, Han F, Shi C, Huang Y, Liu Y and Chang X: Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix. Mol Med Rep 18: 3949-3956, 2018.
APA
Shi, L., Han, F., Shi, C., Huang, Y., Liu, Y., & Chang, X. (2018). Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix. Molecular Medicine Reports, 18, 3949-3956. https://doi.org/10.3892/mmr.2018.9416
MLA
Shi, L., Han, F., Shi, C., Huang, Y., Liu, Y., Chang, X."Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix". Molecular Medicine Reports 18.4 (2018): 3949-3956.
Chicago
Shi, L., Han, F., Shi, C., Huang, Y., Liu, Y., Chang, X."Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix". Molecular Medicine Reports 18, no. 4 (2018): 3949-3956. https://doi.org/10.3892/mmr.2018.9416
Copy and paste a formatted citation
x
Spandidos Publications style
Shi L, Han F, Shi C, Huang Y, Liu Y and Chang X: Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix. Mol Med Rep 18: 3949-3956, 2018.
APA
Shi, L., Han, F., Shi, C., Huang, Y., Liu, Y., & Chang, X. (2018). Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix. Molecular Medicine Reports, 18, 3949-3956. https://doi.org/10.3892/mmr.2018.9416
MLA
Shi, L., Han, F., Shi, C., Huang, Y., Liu, Y., Chang, X."Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix". Molecular Medicine Reports 18.4 (2018): 3949-3956.
Chicago
Shi, L., Han, F., Shi, C., Huang, Y., Liu, Y., Chang, X."Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix". Molecular Medicine Reports 18, no. 4 (2018): 3949-3956. https://doi.org/10.3892/mmr.2018.9416
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