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Article

Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury

  • Authors:
    • Xiaoqin Jiang
    • Jiyun Yang
    • Hedong Li
    • Yi Qu
    • Wenming Xu
    • Haiyan Yu
    • Yu Tong
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Center for Human Molecular Biology and Genetics, Institute of Laboratory Medicine, The Key Laboratory for Human Disease Gene Study of Sichuan, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610041, P.R. China, Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 4595-4602
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    Published online on: September 3, 2018
       https://doi.org/10.3892/mmr.2018.9430
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Abstract

Hypoxic‑ischemic encephalopathy is one of the most notable causes of brain injury in newborns. Cerebral ischemia and reperfusion lead to neuronal damage and neurological disability. In vitro and in vivo analyses have indicated that E3 ubiquitin protein ligase (Huwe1) is important for the process of neurogenesis during brain development; however, the exact biological function and the underlying mechanism of Huwe1 remain controversial. In the present study, neural progenitor cells, L2.3, of which we previously generated from rat E14.5 cortex, were used to investigate the role of Huwe1 and its effects on the downstream N‑Myc‑Delta‑like 3‑Notch1 signaling pathway during oxygen‑glucose deprivation (OGD). To evaluate the role of Huwe1 in L2.3 cells, transduction, cell viability, lactate dehydrogenase, 5‑bromo‑2'deoxyurine incorporation, western blotting and immunocytochemical assays were performed. The results of the present study indicated that Huwe1 rescued L2.3 cells from OGD‑induced insults by inhibiting proliferation and inducing neuronal differentiation. In addition, Huwe1 was suggested to mediate the survival of L2.3 cells by inhibiting the activation of the N‑Myc‑Notch1 signaling pathway. Of note, the effects of Huwe1 on Notch1 signaling were completely abolished by knockdown of N‑Myc, indicating that Huwe1 may require N‑Myc to suppress the activation of the Notch1 signaling in L2.3 cells. The determination of the neuroprotective function of the Huwe1‑N‑Myc‑Notch1 axis may provide insight into novel potential therapeutic targets for the treatment of ischemic stroke.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang X, Yang J, Li H, Qu Y, Xu W, Yu H and Tong Y: Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury. Mol Med Rep 18: 4595-4602, 2018.
APA
Jiang, X., Yang, J., Li, H., Qu, Y., Xu, W., Yu, H., & Tong, Y. (2018). Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury. Molecular Medicine Reports, 18, 4595-4602. https://doi.org/10.3892/mmr.2018.9430
MLA
Jiang, X., Yang, J., Li, H., Qu, Y., Xu, W., Yu, H., Tong, Y."Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury". Molecular Medicine Reports 18.5 (2018): 4595-4602.
Chicago
Jiang, X., Yang, J., Li, H., Qu, Y., Xu, W., Yu, H., Tong, Y."Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury". Molecular Medicine Reports 18, no. 5 (2018): 4595-4602. https://doi.org/10.3892/mmr.2018.9430
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang X, Yang J, Li H, Qu Y, Xu W, Yu H and Tong Y: Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury. Mol Med Rep 18: 4595-4602, 2018.
APA
Jiang, X., Yang, J., Li, H., Qu, Y., Xu, W., Yu, H., & Tong, Y. (2018). Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury. Molecular Medicine Reports, 18, 4595-4602. https://doi.org/10.3892/mmr.2018.9430
MLA
Jiang, X., Yang, J., Li, H., Qu, Y., Xu, W., Yu, H., Tong, Y."Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury". Molecular Medicine Reports 18.5 (2018): 4595-4602.
Chicago
Jiang, X., Yang, J., Li, H., Qu, Y., Xu, W., Yu, H., Tong, Y."Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury". Molecular Medicine Reports 18, no. 5 (2018): 4595-4602. https://doi.org/10.3892/mmr.2018.9430
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