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Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells

  • Authors:
    • Zhiming Wu
    • Huangen Wang
    • Sunyang Fang
    • Chaoyang Xu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Shaoxing Hospital, China Medical University, Shaoxing, Zhejiang 312030, P.R. China, Department of Thyroid Breast Surgery, Shaoxing People's Hospital, Shaoxing, Zhejiang 312030, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4163-4174
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    Published online on: September 3, 2018
       https://doi.org/10.3892/mmr.2018.9443
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Abstract

Endoplasmic reticulum stress (ERS) can be induced by a variety of physiological and pathological factors including oxidative stress, which triggers the unfolded protein response to deal with ERS. Autophagy has been hypothesized to be a means for tumor cells to increase cell survival under conditions of hypoxia, metabolic stress and even chemotherapy. Although they may function independently from each other, there are also interactions between responses to oxidative stress injury induced by pathologic and pharmacological factors. The aim of the present study was to investigate the effects of ERS and autophagy on H2O2‑induced oxidative stress injury in human HepG2 hepatoblastoma cells. It was demonstrated that exposure of HepG2 cells to H2O2 decreased cell viability and increased reactive oxygen species (ROS) levels in a dosage‑dependent manner. In addition, apoptosis and autophagy rates were elevated and reduced following cell exposure to H2O2 + the ERS inducer Tunicamycin (TM), and to H2O2 + the ERS inhibitor Salubrinal (SAL), compared with the cells treated with H2O2 alone, respectively. Further studies revealed that TM enhanced the expression of ERS‑related genes including glucose‑regulated protein‑78/binding immunoglobulin protein, inositol‑requiring kinase‑I and activating transcription factor 6 and C/EBP‑homologous protein 10, which were attenuated by SAL compared with cells exposed to H2O2 alone. The data from the present study also demonstrated that LC3II/LC3‑I and p62, members of autophagy‑related genes, were increased and decreased in cells treated with H2O2 + TM compared with cells treated with H2O2, respectively, indicating that autophagy was stimulated by ERS. Furthermore, a reduction in the levels of pro caspase‑3 and pro caspase‑9, and elevation level of caspase‑12 were observed in cells exposed to H2O2 + TM compared with cells treated with H2O2, respectively, suggesting apoptosis induced by H2O2 was enhanced by ERS or autophagy triggered by H2O2. The above results suggest that the ERS inducer may be a potential target for pharmacological intervention targeted to ERS or autophagy to enhance oxidative stress injury of tumor cells induced by antitumor drugs.
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View References

1 

Benham AM: Protein folding and disulfide bond formation in the eukaryotic cell: Meeting report based on the presentations at the European Network Meeting on Protein Folding and Disulfide Bond Formation 2009 (Elsinore, Denmark). FEBS J. 276:6905–6911. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Kato H and Nishitoh H: Stress responses from the endoplasmic reticulum in cancer. Front Oncol. 5:932015. View Article : Google Scholar : PubMed/NCBI

3 

Henderson KA: Boric acid localization and effects on storage calcium release and the endoplasmic reticulum in prostate cancer cells. Dissertations & Theses-Gradworks. 2009.

4 

Schapansky J, Morissette M, Odero G, Albensi B and Glazner G: Neuregulin beta1 enhances peak glutamate-induced intracellular calcium levels through endoplasmic reticulum calcium release in cultured hippocampal neurons. Can J Physiol Pharmacol. 87:883–891. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Mandl J, Mészáros T, Bánhegyi G and Csala M: Minireview: Endoplasmic reticulum stress: Control in protein, lipid, and signal homeostasis. Mol Endocrinol. 27:384–393. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Rangelaldao R: The unfolded protein response, inflammation, oscillators, and disease: A systems biologyapproach. Endo Reticulum Stress Dis. 2:30–52. 2015.

7 

Richie DL, Feng X, Hartl L, Aimanianda V, Krishnan K, Powers-Fletcher MV, Watson DS, Galande AK, White SM, Willett T, et al: The virulence of the opportunistic fungal pathogen requires cooperation between the endoplasmic reticulum-associated degradation pathway (ERAD) and the unfolded protein response (UPR). Virulence. 2:12–21. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Townsend DM, Manevich Y, He L, Xiong Y, Bowers RR Jr, Hutchens S and Tew KD: Nitrosative-stress induced S-glutathionylation of PDI leads to activation of the unfolded protein response. Cancer Res. 69:7626–7634. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Reddy RK, Mao C, Baumeister P, Austin RC, Kaufman RJ and Lee AS: Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors: Role of ATP binding site in suppression of caspase-7 activation. J Biol Chem. 278:20915–20924. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Bennett HL, Fleming JT, O'Prey J, Ryan KM and Leung HY: Androgens modulate autophagy and cell death via regulation of the endoplasmic reticulum chaperone glucose-regulated protein 78/BiP in prostate cancer cells. Cell Death Dis. 1:e722010. View Article : Google Scholar : PubMed/NCBI

11 

Lu T, Yang W, Wang Z, Hu Z, Zeng X, Yang C, Wang Y, Zhang Y, Li F, Liu Z, Wang D and Ye Z: Knockdown of glucose-regulated protein 78/binding immunoglobulin heavy chain protein expression by asymmetric small interfering RNA induces apoptosis in prostate cancer cells and attenuates migratory capability. Mol Med Rep. 11:2492015. View Article : Google Scholar : PubMed/NCBI

12 

Maiuolo J, Bulotta S, Verderio C, Benfante R and Borgese N: Selective activation of the transcription factor ATF6 mediates endoplasmic reticulum proliferation triggered by a membrane protein. Proc Natl Acad Sci USA. 108:7832–1837. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Umebayashi K, Hirata A, Horiuchi H, Ohta A and Takagi M: Unfolded protein response-induced BiP/Kar2p production protects cell growth against accumulation of misfolded protein aggregates in the yeast endoplasmic reticulum. Eur J Cell Biol. 78:726–738. 1999. View Article : Google Scholar : PubMed/NCBI

14 

Gardner BM and Walter P: Unfolded proteins are Ire1-activating ligands that directly induce the unfolded protein response. Science. 333:1891–1894. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Tang J, Guo YS, Zhang Y, Yu XL, Li L, Huang W, Li Y, Chen B, Jiang JL and Chen ZN: CD147 induces UPR to inhibit apoptosis and chemosensitivity by increasing the transcription of Bip in hepatocellular carcinoma. Cell Death Differ. 19:1779–1790. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Hiss DC and Gabriels GA: Implications of endoplasmic reticulum stress, the unfolded protein response and apoptosis for molecular cancer therapy. Part II: Targeting cell cycle events, caspases, NF-κB and the proteasome. Exp Opin Drug Discov. 4:907–921. 2009. View Article : Google Scholar

17 

Svetlana S, Patricia C, Mnich K, Ayo A, Pakos-Zebrucka K, Patterson JB, Logue SE and Samali A: Endoplasmic reticulum stress-mediated induction of SESTRIN 2 potentiates cell survival. Oncotarget. 7:12254–12266. 2016.PubMed/NCBI

18 

Netherton CL, Parsley JC and Wileman T: African swine fever virus inhibits induction of the stress-induced proapoptotic transcription factor CHOP/GADD153. J Virol. 78:10825–10828. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Moriya S, Miyazawa K, Kawaguchi T, Che XF and Tomoda A: Involvement of endoplasmic reticulum stress-mediated CHOP (GADD153) induction in the cytotoxicity of 2-aminophenoxazine-3-one in cancer cells. Int J Oncol. 39:981–988. 2011.PubMed/NCBI

20 

Tan Y, Dourdin N, Wu C, De VT, Elce JS and Greer PA: Ubiquitous calpains promote caspase-12 and JNK activation during endoplasmic reticulum stress-induced apoptosis. J Biol Chem. 281:16016–16024. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Niso-Santano M, Bravo-San Pedro JM, Gómez-Sánchez R, Climent V, Soler G, Fuentes JM and González-Polo RA: ASK1 overexpression accelerates paraquat-induced autophagy via endoplasmic reticulum stress. Toxicol Sci. 119:1562011. View Article : Google Scholar : PubMed/NCBI

22 

Nakka VP, Prakash-Babu P and Vemuganti R: Crosstalk between endoplasmic reticulum stress, oxidative stress, and autophagy: Potential therapeutic targets for acute CNS Injuries. Mol Neurobiol. 53:532–544. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Devenish RJ and Klionsky DJ: Autophagy: Mechanism and physiological relevance ‘brewed’ from yeast studies. Front Biosci (Schol Ed). 4:1354–1363. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Deegan S, Koryga I, Glynn SA, Gupta S, Gorman AM and Samali A: A close connection between the PERK and IRE arms of the UPR and the transcriptional regulation of autophagy. Biochem Biophys Res Commun. 456:305–311. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Drake KR, Kang M and Kenworthy AK: Nucleocytoplasmic distribution and dynamics of the autophagosome marker EGFP-LC3. PLoS One. 5:e98062010. View Article : Google Scholar : PubMed/NCBI

26 

Tamura H, Shibata M, Koike M, Sasaki M and Uchiyama Y: Atg9A, an autophagy-related membrane protein, is localized in neurons of mouse brain. Neuroscience Research. 68:443–453. 2010. View Article : Google Scholar

27 

Jo YK, Kim SC, Park IJ, Park SJ, Jin DH, Hong SW, Cho DH and Kim JC: Increased expression of ATG10 in colorectal cancer is associated with lymphovascular invasion and lymph node metastasis. PLoS One. 7:e527052012. View Article : Google Scholar : PubMed/NCBI

28 

Chen ZH, Cao JF, Zhou JS, Liu H, Che LQ, Mizumura K, Li W, Choi AM and Shen HH: Interaction of caveolin-1 with ATG12-ATG5 system suppresses autophagy in lung epithelial cells. Am J Physiol Lung Cell Mol Physiol. 306:1016–1025. 2014. View Article : Google Scholar

29 

Hwang S, Maloney NS, Bruinsma MW, Goel G, Duan E, Zhang L, Shrestha B, Diamond MS, Dani A, Sosnovtsev SV, et al: Nondegradative role of Atg5-Atg12/Atg16L1 autophagy protein complex in antiviral activity of interferon gamma. Cell Host Microbe. 11:397–409. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Kung CP, Budina A, Balaburski G, Bergenstock MK and Murphy M: Autophagy in tumor suppression and cancer therapy. Critical Reviews in Eukaryotic Gene Exp. 21:71–100. 2011. View Article : Google Scholar

31 

Lebovitz CB, Bortnik SB and Gorski SM: Here, there be dragons: Charting autophagy-related alterations in human tumors. Clin Cancer Res. 18:1214–1226. 2012. View Article : Google Scholar : PubMed/NCBI

32 

BenYounès A, Tajeddine N, Tailler M, Malik SA, Shen S, Métivier D, Kepp O, Vitale I, Maiuri MC and Kroemer G: A fluorescence-microscopic and cytofluorometric system for monitoring the turnover of the autophagic substrate p62/SQSTM1. Autophagy. 7:883–891. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Komatsu M, Kurokawa H, Waguri S, Taguchi K, Kobayashi A, Ichimura Y, Sou YS, Ueno I, Sakamoto A, Tong KI, et al: The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. Nat Cell Biol. 12:213–223. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Kundu M: ULK1, Mammalian Target of Rapamycin, and Mitochondria: Linking Nutrient Availability and Autophagy. Antioxid Redox Signal. 14:1953–1958. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Cheong H and Klionsky DJ: Dual role of Atg1 in regulation of autophagy-specific PAS assembly in Saccharomyces cerevisiae. Autophagy. 4:724–726. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Luciani MF, Giusti C, Harms B, Oshima Y, Kikuchi H, Kubohara Y and Golstein P: Atg1 allows second-signaled autophagic cell death in Dictyostelium. Autophagy. 7:501–508. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Yorimitsu T, Nair U, Yang Z and Klionsky DJ: Endoplasmic reticulum stress triggers autophagy. J Biol Chem. 281:30299–30304. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Tekirdag KA, Korkmaz G, Ozturk DG, Agami R and Gozuacik D: MIR181A regulates starvation- and rapamycin-induced autophagy through targeting of ATG5. Autophagy. 9:374–385. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Lopez-Terrada D, Cheung SW, Finegold MJ and Knowles BB: Hep G2 is a hepatoblastoma-derived cell line. Hum Pathol. 40:1512–1515. 2009. View Article : Google Scholar : PubMed/NCBI

40 

Kenneth J and Livak TD: Analysis of relative gene expression data using rea l-time quantitative PCR a nd the 2(-Delta Delta C(T)) method. Method. 25:402–408. 2001. View Article : Google Scholar

41 

Simasi J, Schubert A, Oelkrug C, Gillissen A and Nieber K: Primary and secondary resistance to tyrosine kinase inhibitors in lung cancer. Anticancer Res. 34:2841–2850. 2014.PubMed/NCBI

42 

Quintás-Cardama A, Kantarjian HM and Cortes JE: Mechanisms of primary and secondary resistance to imatinib in chronic myeloid leukemia. Cancer Control. 16:122–131. 2009. View Article : Google Scholar : PubMed/NCBI

43 

Schleicher SM, Moretti L, Varki V and Lu B: Progress in the unraveling of the endoplasmic reticulum stress/autophagy pathway and cancer: Implications for future therapeutic approaches. Drug Resist Updat. 13:79–86. 2010. View Article : Google Scholar : PubMed/NCBI

44 

Li T, Su L, Zhong N, Hao X, Zhong D, Singhal S and Liu X: Salinomycin induces cell death with autophagy through activation of endoplasmic reticulum stress in human cancer cells. Autophagy. 9:1057–1068. 2013. View Article : Google Scholar : PubMed/NCBI

45 

Su CC: Tanshinone IIA could inhibit pancreatic cancer BxPC-3 cells through increasing PERK, ATF6, Caspase-12 and CHOP expression to induce apoptosis. J Biom Sci Eng. 8:149–159. 2015. View Article : Google Scholar

46 

Liu H, Nishitoh H, Ichijo H and Kyriakis JM: Activation of apoptosis signal-regulating kinase 1 (ASK1) by tumor necrosis factor receptor-associated factor 2 requires prior dissociation of the ASK1 inhibitor thioredoxin. Mol Cell Biol. 20:2198–2208. 2000. View Article : Google Scholar : PubMed/NCBI

47 

Nakagawa T and Yuan J: Cross-talk between two cysteine protease families activation of caspase-12 by calpain in apoptosis. J Cell Biol. 150:887–894. 2000. View Article : Google Scholar : PubMed/NCBI

48 

Morishima N, Nakanishi K, Takenouchi H, Shibata T and Yasuhiko Y: An Endoplasmic Reticulum Stress-specific Caspase Cascade in Apoptosis cytochrome c-independent activation of caspase-9 by caspase-12. J Biol Chem. 277:34287–34294. 2002. View Article : Google Scholar : PubMed/NCBI

49 

Bae JY and Park HH: Purification and characterization of a ubiquitin-like system for autophagosome formation. J Microbiol Biotechnol. 20:1647–1652. 2010.PubMed/NCBI

50 

Satoo K, Noda NN, Kumeta H, Fujioka Y, Mizushima N, Ohsumi Y and Inagaki F: The structure of Atg4B-LC3 complex reveals the mechanism of LC3 processing and delipidation during autophagy. EMBO J. 28:1341–1350. 2009. View Article : Google Scholar : PubMed/NCBI

51 

Tanida I, Minematsuikeguchi N, Ueno T and Kominami E: Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy. 1:84–91. 2005. View Article : Google Scholar : PubMed/NCBI

52 

Sailaja GS, Praveen B, Bharathi G, Chetty C, Gogineni VR, Velpula KK, Gondi CS and Rao JS: The secreted protein acidic and rich in cysteine (SPARC) induces endoplasmic reticulum stress leading to autophagy-mediated apoptosis in neuroblastoma. Int J Oncol. 42:188–196. 2013. View Article : Google Scholar : PubMed/NCBI

53 

Jiang Q, Li F, Shi K, Wu P, An J, Yang Y and Xu C: Involvement of p38 in signal switching from autophagy to apoptosis via the PERK/eIF2α/ATF4 axis in selenite-treated NB4 cells. Cell Death Dis. 5:e12702014. View Article : Google Scholar : PubMed/NCBI

54 

Kamada Y: Prime-numbered Atg proteins act at the primary step in autophagy: Unphosphorylatable Atg13 can induce autophagy without TOR inactivation. Autophagy. 6:415–416. 2010. View Article : Google Scholar : PubMed/NCBI

55 

Saito A, Ochiai K, Kondo S, Tsumagari K, Murakami T, Cavener DR and Imaizumi K: Endoplasmic reticulum stress response mediated by the PERK-eIF2(alpha)-ATF4 pathway is involved in osteoblast differentiation induced by BMP2. J Biol Chem. 286:4809–4818. 2011. View Article : Google Scholar : PubMed/NCBI

56 

Kimura S, Noda T and Yoshimori T: Dynein-dependent movement of autophagosomes mediates efficient encounters with lysosomes. Cell Struct Funct. 33:109–122. 2008. View Article : Google Scholar : PubMed/NCBI

57 

Lee H, Noh JY, Oh Y, Kim Y, Chang JW, Chung CW, Lee ST, Kim M, Ryu H and Jung YK: IRE1 plays an essential role in ER stress-mediated aggregation of mutant huntingtin via the inhibition of autophagy flux. Hum Mol Genet. 21:101–114. 2012. View Article : Google Scholar : PubMed/NCBI

58 

Zhang C, Kawauchi J, Adachi MT, Hashimoto Y, Oshiro S, Aso T and Kitajima S: Activation of JNK and Transcriptional Repressor ATF3/LRF1 through the IRE1/TRAF2 pathway is implicated in human vascular endothelial cell death by homocysteine. Biochem Biophys Res Commun. 289:718–724. 2001. View Article : Google Scholar : PubMed/NCBI

59 

Shvets E, Fass E, Scherz-Shouval R and Elazar Z: The N-terminus and Phe52 residue of LC3 recruit p62/SQSTM1 into autophagosomes. J Cell Sci. 121:2685–2695. 2008. View Article : Google Scholar : PubMed/NCBI

60 

Liu WJ, Ye L, Huang WF, Guo LJ, Xu ZG, Wu HL, Yang C and Liu HF: p62 links the autophagy pathway and the ubiqutin-proteasome system upon ubiquitinated protein degradation. Cell Mol Biol Lett. 21:292016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu Z, Wang H, Fang S and Xu C: Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells. Mol Med Rep 18: 4163-4174, 2018.
APA
Wu, Z., Wang, H., Fang, S., & Xu, C. (2018). Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells. Molecular Medicine Reports, 18, 4163-4174. https://doi.org/10.3892/mmr.2018.9443
MLA
Wu, Z., Wang, H., Fang, S., Xu, C."Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells". Molecular Medicine Reports 18.5 (2018): 4163-4174.
Chicago
Wu, Z., Wang, H., Fang, S., Xu, C."Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells". Molecular Medicine Reports 18, no. 5 (2018): 4163-4174. https://doi.org/10.3892/mmr.2018.9443
Copy and paste a formatted citation
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Spandidos Publications style
Wu Z, Wang H, Fang S and Xu C: Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells. Mol Med Rep 18: 4163-4174, 2018.
APA
Wu, Z., Wang, H., Fang, S., & Xu, C. (2018). Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells. Molecular Medicine Reports, 18, 4163-4174. https://doi.org/10.3892/mmr.2018.9443
MLA
Wu, Z., Wang, H., Fang, S., Xu, C."Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells". Molecular Medicine Reports 18.5 (2018): 4163-4174.
Chicago
Wu, Z., Wang, H., Fang, S., Xu, C."Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells". Molecular Medicine Reports 18, no. 5 (2018): 4163-4174. https://doi.org/10.3892/mmr.2018.9443
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