Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November-2018 Volume 18 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2018 Volume 18 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury

  • Authors:
    • Yang Liu
    • Wanting Cao
    • Xiangbo Kong
    • Jie Li
    • Xueying Chen
    • Yaping Ge
    • Wanzhen Zhong
    • Silian Fang
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, The Sixth Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510655, P.R. China, Department of Stomatology, Sun Yat‑Sen Memorial Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4219-4228
    |
    Published online on: September 4, 2018
       https://doi.org/10.3892/mmr.2018.9449
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Osteoradionecrosis of the jaws (ORNJ) is a complication of oral and maxillofacial malignancy that arises following radiotherapy; progressive jaw necrosis severely decreases the quality of life of patients. Human bone marrow mesenchymal stem cells (hBMMSCs) are a cell type with self‑renewal and pluripotent differentiation potential in the bone marrow stroma. These cells are associated with bone tissue regeneration and are one of the primary cell types affected by bone tissue radiation injury. α‑2‑macroglobulin (α2M) is a glycoprotein‑rich macromolecule that interacts with cytokines, growth factors and hormones to serve a variety of biological roles. In addition, α2M possesses radio‑protective effects. The aim of the present study was to investigate whether α2M has protective effects against radiation injury of hBMMSCs. Cell counting kit‑8 and colony formation assays were used to monitor cell proliferation. Western blot analysis and reverse transcription‑quantitative polymerase chain reaction were used to detect Beclin1, microtubule‑associated protein 1A/1B, sex determining region Y, Nanog, runt‑related transcription factor 2, osteoglycin and manganese superoxide dismutase expression. The formation of calcium nodules was evaluated by Alizarin red staining after osteogenic induction. Flow cytometric analysis of Annexin‑V and propidium iodide double staining was used to detect changes in apoptosis rate. Alkaline phosphatase and superoxide dismutase activity were determined using colorimetric assays. Reactive oxygen species levels were detected using 2',7'‑dichlorodihydrofluorescein diacetate. The results of the present study revealed that α2M increased the rate of proliferation, reduced autophagy, alleviated pluripotent differentiation injury, increased the osteogenic differentiation ability and decreased the rate of apoptosis in hBMMSCs following irradiation via an antioxidative pathway. In conclusion, α2M exhibited protective effects against radiation injury in hBMMSCs and may be considered a potential therapeutic agent for the prevention and treatment of ORNJ.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Bots WTC, van den Bosch S, Zwijnenburg EM, Dijkema T, van den Broek GB, Weijs WLJ, Verhoef LCG and Kaanders JHAM: Reirradiation of head and neck cancer: Long-term disease control and toxicity. Head Neck. 39:1122–1130. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Nadella KR, Kodali RM, Guttikonda LK and Jonnalagadda A: Osteoradionecrosis of the Jaws: Clinico-therapeutic management: A literature review and update. J Maxillofac Oral Surg. 14:891–901. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Dhanda J, Pasquier D, Newman L and Shaw R: Current concepts in osteoradionecrosis after head and neck radiotherapy. Clin Oncol (R Coll Radiol). 28:459–466. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Moroni L and Fornasari PM: Human mesenchymal stem cells: A bank perspective on the isolation, characterization and potential of alternative sources for the regeneration of musculoskeletal tissues. J Cell Physiol. 228:680–687. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Baker N, Boyette LB and Tuan RS: Characterization of bone marrow-derived mesenchymal stem cells in aging. Bone. 70:37–47. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Wang Y, Zhu G, Wang J and Chen J: Irradiation alters the differentiation potential of bone marrow mesenchymal stem cells. Mol Med Rep. 13:213–223. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Fang S, Chen W, Wang S, Kong X, Sun X, Ge Y and Hu K: Proteomic Analysis of Serum in patients with Osteoradionecrosis of the Jaws. Int Dental J. 62:242012.

8 

Fang S, Kong X, Chen W, Chen Y, He F, Ge Y, Sun X and Wang S: Identification of differential proteins that related to bone metabolism in the serum of ORNJ patients. Int Dental J. 62:252012.

9 

Chen X, Kong X, Cao W, Ge Y, Wang S, Zhang Z, Li H, Liu Y and Fang S: The detection of serum and urine levels of alpha 2-Macroglobulin in nasopharyngeal carcinoma patients receiving late period radiotherapy and its significance. Int J Dentistry Oral Sci. 5:611–617. 2018.

10 

Rehman AA, Ahsan H and Khan FH: α2-macroglobulin: A physiological guardian. J Cell Physiol. 228:1665–1675. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Rehman AA, Zaman M, Zia MK, Ahsan H, Khan RH and Khan FH: Conformational behavior of alpha-2-macroglobulin: Aggregation and inhibition induced by TFE. Int J Biol Macromol. 104:539–546. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Chen X, Kong X, Zhang Z, Chen W, Chen J, Li H, Cao W, Ge Y and Fang S: Alpha-2-macroglobulin as a radioprotective agent: A review. Chin J Cancer Res. 26:611–621. 2014.PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Lyons A and Ghazali N: Osteoradionecrosis of the jaws: Current understanding of its pathophysiology and treatment. Br J Oral Maxillofac Surg. 46:653–660. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Wissing MD: Chemotherapy- and irradiation-induced bone loss in adults with solid tumors. Curr Osteoporos Rep. 13:140–145. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Bogojević D, Poznanović G, Grdović N, Grigorov I, Vidaković M, Dinić S and Mihailović M: Administration of rat acute-phase protein α(2)-macroglobulin before total-body irradiation initiates cytoprotective mechanisms in the liver. Radiat Environ Biophys. 50:167–179. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Aizman I, Holland WS, Yang C and Bates D: αSMA expression in large colonies of Colony-forming units-fibroblast as an early predictor of bone marrow MSC expandability. Cell Med. 8:79–85. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Liang DH, El-Zein R and Dave B: Autophagy Inhibition to Increase Radiosensiti-zation in breast cancer. J Nucl Med Radiat Ther. 6:2542015. View Article : Google Scholar : PubMed/NCBI

19 

Koukourakis MI, Mitrakas AG and Giatromanolaki A: Therapeutic interactions of autophagy with radiation and temozolomide in glioblastoma: Evidence and issues to resolve. Br J Cancer. 114:485–496. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Kim M, Fekadu J, Maronde E and Rami A: Alleviation of autophagy by knockdown of Beclin-1 enhances susceptibility of hippocampal neurons to proapoptotic signals indeced by amino acid starvation. Histochem Cell Biol. 139:99–108. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Go MJ, Takenaka C and Ohgushi H: Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells maintains their expansion and differentiation capabilities. Exp Cell Res. 314:1147–1154. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Heffernan C, Sumer H and Verma PJ: Generation of clinically relevant ‘induced pluripotent stem’ (iPS) cells. J Stem Cells. 6:109–127. 2011.PubMed/NCBI

23 

Millán JL: The role of phosphatases in the initiation of skeletal mineralization. Calcif Tissue Int. 93:299–306. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Halling Linder C, Enander K and Magnusson P: Glycation contributes to interaction between human bone alkaline phosphatase and collagen type I. Calcif Tissue Int. 98:284–293. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Henriquez B, Hepp M, Merino P, Sepulveda H, van Wijnen AJ, Lian JB, Stein GS, Stein JL and Montecino M: C/EBPβ binds the P1 promoter of the Runx2 gene and up-regulates Runx2 transcription in osteoblastic cells. J Cell Physiol. 226:3043–3052. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Qin X, Jiang Q, Matsuo Y, Kawane T, Komori H, Moriishi T, Taniuchi I, Ito K, Kawai Y, Rokutanda S, et al: Cbfb regulates bone development by stabilizing runx family proteins. J Bone Miner Res. 30:706–714. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Tanaka K, Matsumoto E, Higashimaki Y, Katagiri T, Sugimoto T, Seino S and Kaji H: Role of osteoglycin in the linkage between muscle and bone. J Biol Chem. 287:11616–11628. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Gregory CA, Gunn WG, Peister A and Prockop DJ: An Alizarin red-based assay of mineralization by adherent cells in culture: Comparison with cetylpyridinium chloride extraction. Anal Biochem. 329:77–84. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Sakuma S, Abe M, Kohda T and Fujimoto Y: Hydrogen peroxide generated by xanthine/xanthine oxidase system represses the proliferation of colorectal cancer cell line Caco-2. J Clin Biochem Nutr. 56:15–19. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Ivanov VE, Usacheva AM, Chernikov AV, Bruskov VI and Gudkov SV: Formation of long-lived reactive species of blood serum proteins induced by low-intensity irradiation of helium-neon laser and their involvement in the generation of reactive oxygen species. J Photochem Photobiol B. 176:36–43. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Wu J, Liu S, Meng H, Qu T, Fu S, Wang Z, Yang J, Jin D and Yu B: Neuropeptide Y enhances proliferation and prevents apoptosis in rat bone marrow stromal cells in association with activation of the Wnt/β-catenin pathway in vitro. Stem Cell Res. 21:74–84. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Deng B, Jiang H, Zeng K, Liang Y, Wu Y and Yang Y: Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells. Mol Med Rep. 15:3499–3506. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Hou J, Han ZP, Jing YY, Yang X, Zhang SS, Sun K, Hao C, Meng Y, Yu FH, Liu XQ, et al: Autophagy prevents irradiation injury and maintains stemness through decreasing ROS generation in mesenchymal stem cells. Cell Death Dis. 4:e8442013. View Article : Google Scholar : PubMed/NCBI

34 

M Fetherolf M, Boyd SD, Winkler DD and Winge DR: Oxygen-dependent activation of Cu, Zn-superoxide dismutase-1. Metallomics. 9:1047–1059. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Cao W, Kong X, Li J, Chen X, Ge Y, Zhong W and Fang S: Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury. Mol Med Rep 18: 4219-4228, 2018.
APA
Liu, Y., Cao, W., Kong, X., Li, J., Chen, X., Ge, Y. ... Fang, S. (2018). Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury. Molecular Medicine Reports, 18, 4219-4228. https://doi.org/10.3892/mmr.2018.9449
MLA
Liu, Y., Cao, W., Kong, X., Li, J., Chen, X., Ge, Y., Zhong, W., Fang, S."Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury". Molecular Medicine Reports 18.5 (2018): 4219-4228.
Chicago
Liu, Y., Cao, W., Kong, X., Li, J., Chen, X., Ge, Y., Zhong, W., Fang, S."Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury". Molecular Medicine Reports 18, no. 5 (2018): 4219-4228. https://doi.org/10.3892/mmr.2018.9449
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Cao W, Kong X, Li J, Chen X, Ge Y, Zhong W and Fang S: Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury. Mol Med Rep 18: 4219-4228, 2018.
APA
Liu, Y., Cao, W., Kong, X., Li, J., Chen, X., Ge, Y. ... Fang, S. (2018). Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury. Molecular Medicine Reports, 18, 4219-4228. https://doi.org/10.3892/mmr.2018.9449
MLA
Liu, Y., Cao, W., Kong, X., Li, J., Chen, X., Ge, Y., Zhong, W., Fang, S."Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury". Molecular Medicine Reports 18.5 (2018): 4219-4228.
Chicago
Liu, Y., Cao, W., Kong, X., Li, J., Chen, X., Ge, Y., Zhong, W., Fang, S."Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury". Molecular Medicine Reports 18, no. 5 (2018): 4219-4228. https://doi.org/10.3892/mmr.2018.9449
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team