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Article

miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4

  • Authors:
    • Hongwei Xu
    • Quan Wen
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, General Internal Medicine Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 4621-4628
    |
    Published online on: September 4, 2018
       https://doi.org/10.3892/mmr.2018.9454
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Abstract

Accumulating evidence indicates that microRNAs (miRs) are important regulators in a number of types of human cancer, including non‑small cell lung cancer (NSCLC). The function of miR‑3120‑5p in NSCLC remains unclear. In the present study, it was demonstrated that miR‑3120‑5p was significantly upregulated in NSCLC tissues. Additionally, miR‑3120‑5p expression level was positively associated with NSCLC metastasis and tumor, node and metastasis stage. Furthermore, it was demonstrated that miR‑3120‑5p exhibited potential as an indicator of NSCLC for use in diagnosis. Through functional experiments, it was demonstrated that overexpression of miR‑3120‑5p promoted the proliferation, colony formation and invasion of NSCLC cells. miR‑3120‑5p overexpression significantly promoted cell cycle progression. Mechanistically, it was demonstrated that Krueppel‑like factor 4 (KLF4) was a target of miR‑3120‑5p in NSCLC cells. Overexpression of miR‑3120‑5p repressed the expression of KLF4 in A549 and H460 cells. Furthermore, it was demonstrated that KLF4 was downregulated in NSCLC tissues and cell lines. Overexpression of KLF4 significantly reversed the effects of miR‑3120‑5p on NSCLC cell proliferation and invasion. In conclusion, the present study demonstrated that miR‑3120‑5p promoted NSCLC progression by directly targeting KLF4.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Chen KD, Huang KT, Lin CC, Weng WT, Hsu LW, Goto S, Nakano T, Lai CY, Kung CP, Chiu KW, et al: MicroRNA-27b enhances the hepatic regenerative properties of adipose-derived mesenchymal stem cells. Mol Ther Nucleic Acids. 5:e2852016. View Article : Google Scholar : PubMed/NCBI

3 

Karlsen TA, de Souza GA, Odegaard B, Engebretsen L and Brinchmann JE: microRNA-140 inhibits inflammation and stimulates chondrogenesis in a model of interleukin 1β-induced Osteoarthritis. Mol Ther Nucleic Acids. 5:e3732016. View Article : Google Scholar : PubMed/NCBI

4 

Lemjabbar-Alaoui H, Hassan OU, Yang YW and Buchanan P: Lung cancer: Biology and treatment options. Biochim. Biophys Acta. 1856:189–210. 2015.

5 

Huang K, Dong X, Sui C, Hu D, Xiong T, Liao S and Zhang H: MiR-223 suppresses endometrial carcinoma cells proliferation by targeting IGF-1R. Am J Transl Res. 6:841–849. 2014.PubMed/NCBI

6 

Huang X, Huang M, Kong L and Li Y: miR-372 suppresses tumour proliferation and invasion by targeting IGF2BP1 in renal cell carcinoma. Cell Prolif. 48:593–599. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Shukla GC, Singh J and Barik S: MicroRNAs: Processing, maturation, target recognition and regulatory functions. Mol Cell Pharmacol. 3:83–92. 2011.PubMed/NCBI

8 

Kim G, An HJ, Lee MJ, Song JY, Jeong JY, Lee JH and Jeong HC: Hsa-miR-1246 and hsa-miR-1290 are associated with stemness and invasiveness of non-small cell lung cancer. Lung Cancer. 91:15–22. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Yang Z, He J, Gao P, Niu Y, Zhang J, Wang L, Liu M, Wei X, Liu C, Zhang C, et al: miR-769-5p suppressed cell proliferation, migration and invasion by targeting TGFBR1 in non-small cell lung carcinoma. Oncotarget. 8:113558–113570. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Li G, Wu F, Yang H, Deng X and Yuan Y: MiR-9-5p promotes cell growth and metastasis in non-small cell lung cancer through the repression of TGFBR2. Biomed Pharmacother. 96:1170–1178. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Wang M, Meng B and Liu Y, Yu J, Chen Q and Liu Y: MiR-124 inhibits growth and enhances radiation-induced apoptosis in non-small cell lung cancer by inhibiting STAT3. Cell Physiol Biochem. 44:2017–2028. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Lu G, Fu D, Jia C, Chai L, Han Y, Liu J, Wu T, Xie R, Chang Z, Yang H, et al: Reduced miR-105-1 levels are associated with poor survival of patients with non-small cell lung cancer. Oncol Lett. 14:7842–7848. 2017.PubMed/NCBI

13 

Zhou Q, Huang SX, Zhang F, Li SJ, Liu C, Xi YY, Wang L, Wang X, He QQ, Sun CC and Li DJ: MicroRNAs: A novel potential biomarker for diagnosis and therapy in patients with non-small cell lung cancer. Cell Prolif. 50:2017. View Article : Google Scholar :

14 

Hongdan L and Feng L: miR-3120-5p promotes colon cancer stem cell stemness and invasiveness through targeting Axin2. Biochem Biophys Res Commun. 496:302–308. 2018. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Cai Y, Ruan J, Yao X, Zhao L and Wang B: MicroRNA-187 modulates epithelial-mesenchymal transition by targeting PTRF in non-small cell lung cancer. Oncol Rep. 37:2787–2794. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Cheng R, Lu C, Zhang G, Zhang G and Zhao G: Overexpression of miR-203 increases the sensitivity of NSCLC A549/H460 cell lines to cisplatin by targeting Dickkopf-1. Oncol Rep. 37:2129–2136. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Xu G, Shao G, Pan Q, Sun L, Zheng D, Li M, Li N, Shi H and Ni Y: MicroRNA-9 regulates non-small cell lung cancer cell invasion and migration by targeting eukaryotic translation initiation factor 5A2. Am J Transl Res. 9:478–488. 2017.PubMed/NCBI

19 

Chen S, Jiang S, Hu F, Xu Y, Wang T and Mei Q: Foxk2 inhibits non-small cell lung cancer epithelial-mesenchymal transition and proliferation through the repression of different key target genes. Oncol Rep. 37:2335–2347. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Zhijun Z and Jingkang H: MicroRNA-520e suppresses non-small-cell lung cancer cell growth by targeting Zbtb7a-mediated Wnt signaling pathway. Biochem. Biophys Res Commun. 486:49–56. 2017. View Article : Google Scholar

21 

Shi L, Xu Z, Wu G, Chen X, Huang Y, Wang Y, Jiang W and Ke B: Up-regulation of miR-146a increases the sensitivity of non-small cell lung cancer to DDP by downregulating cyclin J. BMC Cancer. 17:1382017. View Article : Google Scholar : PubMed/NCBI

22 

Shi ZM, Wang L, Shen H, Jiang CF, Ge X, Li DM, Wen YY, Sun HR, Pan MH, Li W, et al: Downregulation of miR-218 contributes to epithelial-mesenchymal transition and tumor metastasis in lung cancer by targeting Slug/ZEB2 signaling. Oncogene. 36:2577–2588. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Ye B, Liu B, Hao L, Zhu X, Yang L, Wang S, Xia P, Du Y, Meng S, Huang G, et al: Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice. Nat Commun. 9:12612018. View Article : Google Scholar : PubMed/NCBI

24 

Li S, Huang L, Gu J, Wu J, Ou W, Feng J, Liu B, Xu X and Zhou Y: Restoration of KLF4 Inhibits Invasion and Metastases of Lung Adenocarcinoma through Suppressing MMP2. J Cancer. 8:3480–3489. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Wu LP, Wu J, Shang A, Yang M, Li LL, Yu J, Xu LR, Wang CB, Wang WW, Zhu JJ and Lu WY: miR-124 inhibits progression of hepatocarcinoma by targeting KLF4 and promises a novel diagnostic marker. Artif Cells Nanomed Biotechnol. Dec 18–2017.(Epub ahead of print). View Article : Google Scholar

26 

Hu W, Hofstetter WL, Li H, Zhou Y, He Y, Pataer A, Wang L, Xie K, Swisher SG and Fang B: Putative tumor-suppressive function of Kruppel-like factor 4 in primary lung carcinoma. Clin Cancer Res. 15:5688–5695. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Zhou Y, Hofstetter WL, He Y, Hu W, Pataer A, Wang L, Wang J, Zhou Y, Yu L, Fang B and Swisher SG: KLF4 inhibition of lung cancer cell invasion by suppression of SPARC expression. Cancer Biol Ther. 9:507–513. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Fadous-Khalife MC, Aloulou N, Jalbout M, Hadchity J, Aftimos G, Paris F and Hadchity E: Kruppel-like factor 4: A new potential biomarker of lung cancer. Mol Clin Oncol. 5:35–40. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu H and Wen Q: miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4. Mol Med Rep 18: 4621-4628, 2018.
APA
Xu, H., & Wen, Q. (2018). miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4. Molecular Medicine Reports, 18, 4621-4628. https://doi.org/10.3892/mmr.2018.9454
MLA
Xu, H., Wen, Q."miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4". Molecular Medicine Reports 18.5 (2018): 4621-4628.
Chicago
Xu, H., Wen, Q."miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4". Molecular Medicine Reports 18, no. 5 (2018): 4621-4628. https://doi.org/10.3892/mmr.2018.9454
Copy and paste a formatted citation
x
Spandidos Publications style
Xu H and Wen Q: miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4. Mol Med Rep 18: 4621-4628, 2018.
APA
Xu, H., & Wen, Q. (2018). miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4. Molecular Medicine Reports, 18, 4621-4628. https://doi.org/10.3892/mmr.2018.9454
MLA
Xu, H., Wen, Q."miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4". Molecular Medicine Reports 18.5 (2018): 4621-4628.
Chicago
Xu, H., Wen, Q."miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4". Molecular Medicine Reports 18, no. 5 (2018): 4621-4628. https://doi.org/10.3892/mmr.2018.9454
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