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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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November-2018 Volume 18 Issue 5

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Article

Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells

  • Authors:
    • Ling‑Xin Meng
    • Yu‑Xiu Zheng
    • Mao‑Lei He
    • Xiao‑Ming Zhou
    • Shu‑Yan Sun
    • Zhao‑Jun Ding
    • Qin Meng
    • Bing‑Cheng Li
    • Yan‑Wei Sun
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, People's Hospital of Rizhao, Rizhao, Shandong 276826, P.R. China, Department of Pathology, People's Hospital of Rizhao, Rizhao, Shandong 276826, P.R. China
  • Pages: 4571-4576
    |
    Published online on: September 5, 2018
       https://doi.org/10.3892/mmr.2018.9461
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Abstract

Previous studies have identified that perilipin-1 (PLIN1) is a highly specific marker for liposarcoma. However, its functions have yet to be fully elucidated. The aim of the present study was to investigate the potential role of PLIN1 in the proliferation, migration and apoptosis of liposarcoma cells. Short hairpin RNA was designed to inhibit PLIN1 levels. Cell proliferation was monitored by Cell Counting Kit‑8 assay and cell migration determined by wound healing assay. Flow cytometry was performed to assess the cell cycle distributions and apoptosis in liposarcoma cells. The results demonstrated that the expression of PLIN1 was significantly upregulated in liposarcoma tumor tissues compared with normal adipose tissues. Silencing of PLIN1 by short hairpin RNA significantly inhibited proliferation and migration and induced G1 phase cell cycle arrest and apoptosis in liposarcoma cell lines. It was identified that PLIN1 serves a crucial role in the pathogenesis and progression of liposarcoma and may be a potential therapeutic target for its clinical management.
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Copy and paste a formatted citation
Spandidos Publications style
Meng LX, Zheng YX, He ML, Zhou XM, Sun SY, Ding ZJ, Meng Q, Li BC and Sun YW: Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells. Mol Med Rep 18: 4571-4576, 2018.
APA
Meng, L., Zheng, Y., He, M., Zhou, X., Sun, S., Ding, Z. ... Sun, Y. (2018). Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells. Molecular Medicine Reports, 18, 4571-4576. https://doi.org/10.3892/mmr.2018.9461
MLA
Meng, L., Zheng, Y., He, M., Zhou, X., Sun, S., Ding, Z., Meng, Q., Li, B., Sun, Y."Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells". Molecular Medicine Reports 18.5 (2018): 4571-4576.
Chicago
Meng, L., Zheng, Y., He, M., Zhou, X., Sun, S., Ding, Z., Meng, Q., Li, B., Sun, Y."Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells". Molecular Medicine Reports 18, no. 5 (2018): 4571-4576. https://doi.org/10.3892/mmr.2018.9461
Copy and paste a formatted citation
x
Spandidos Publications style
Meng LX, Zheng YX, He ML, Zhou XM, Sun SY, Ding ZJ, Meng Q, Li BC and Sun YW: Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells. Mol Med Rep 18: 4571-4576, 2018.
APA
Meng, L., Zheng, Y., He, M., Zhou, X., Sun, S., Ding, Z. ... Sun, Y. (2018). Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells. Molecular Medicine Reports, 18, 4571-4576. https://doi.org/10.3892/mmr.2018.9461
MLA
Meng, L., Zheng, Y., He, M., Zhou, X., Sun, S., Ding, Z., Meng, Q., Li, B., Sun, Y."Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells". Molecular Medicine Reports 18.5 (2018): 4571-4576.
Chicago
Meng, L., Zheng, Y., He, M., Zhou, X., Sun, S., Ding, Z., Meng, Q., Li, B., Sun, Y."Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells". Molecular Medicine Reports 18, no. 5 (2018): 4571-4576. https://doi.org/10.3892/mmr.2018.9461
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