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Article Open Access

Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro

  • Authors:
    • Xiaoju Li
    • Huixiao Yang
    • Zijiian Zhang
    • Zhonghai Yan
    • Huling Lv
    • Yan Zhang
    • Bin Wu
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The People's Hospital of Longhua, Shenzhen, Guangdong 518109, P.R. China, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatological Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China, Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX 79905, USA, Department of General Therapy Dentistry, Stomatology Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Pages: 4477-4485
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    Published online on: September 10, 2018
       https://doi.org/10.3892/mmr.2018.9472
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Abstract

The purpose of the present study was to evaluate the effects of platelet‑rich fibrin (PRF) exudate on the proliferation, osteogenic differentiation and mineralization of human periodontal ligament cells (hPDLCs) in vitro. In the present study PRF was obtained with permission, from the peripheral blood of healthy donors and PRF exudates were collected on the 7th day of incubation. hPDLCs were obtained from healthy premolars, cultured by a tissue explant method and identified with anti‑vimentin and anti‑cytokeratin antibodies. PRF exudates were added to hPDLCs in different concentrations to evaluate cell proliferation and osteogenic differentiation. The proliferation of hPDLCs was measured using a colorimetric assay. Osteogenic differentiation and mineralization were determined by Alizarin red staining, alkaline phosphatase activity (ALP), western blotting and reverse transcription‑quantitative polymerase chain reaction. Cell proliferation was enhanced by addition of the PRF exudate, which also promoted the formation of mineralized matrix nodules and upregulated ALP activity and osteoblast‑associated levels of osteocalcin, runt‑related transcription factor and osterix gene expression. As these stimulatory effects occurred in a dose‑dependent manner, it was concluded that high concentrations of the PRF exudate served an essential role in the proliferation, osteogenic differentiation and mineralization of hPDLCs in vitro. The present study demonstrated that PRF exudate enhanced hPDLC proliferation, induced the osteoblastic differentiation of hPDLCs into mineralized tissue‑formation cells in vitro, and may therefore provide potential benefits for periodontal tissue engineering; contributing to the primary processes of periodontal tissue regeneration. From the perspective of both economics and biology, PRF has greater clinical benefits than analogous growth factors.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Yang H, Zhang Z, Yan Z, Lv H, Zhang Y and Wu B: Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro. Mol Med Rep 18: 4477-4485, 2018.
APA
Li, X., Yang, H., Zhang, Z., Yan, Z., Lv, H., Zhang, Y., & Wu, B. (2018). Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro. Molecular Medicine Reports, 18, 4477-4485. https://doi.org/10.3892/mmr.2018.9472
MLA
Li, X., Yang, H., Zhang, Z., Yan, Z., Lv, H., Zhang, Y., Wu, B."Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro". Molecular Medicine Reports 18.5 (2018): 4477-4485.
Chicago
Li, X., Yang, H., Zhang, Z., Yan, Z., Lv, H., Zhang, Y., Wu, B."Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro". Molecular Medicine Reports 18, no. 5 (2018): 4477-4485. https://doi.org/10.3892/mmr.2018.9472
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Yang H, Zhang Z, Yan Z, Lv H, Zhang Y and Wu B: Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro. Mol Med Rep 18: 4477-4485, 2018.
APA
Li, X., Yang, H., Zhang, Z., Yan, Z., Lv, H., Zhang, Y., & Wu, B. (2018). Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro. Molecular Medicine Reports, 18, 4477-4485. https://doi.org/10.3892/mmr.2018.9472
MLA
Li, X., Yang, H., Zhang, Z., Yan, Z., Lv, H., Zhang, Y., Wu, B."Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro". Molecular Medicine Reports 18.5 (2018): 4477-4485.
Chicago
Li, X., Yang, H., Zhang, Z., Yan, Z., Lv, H., Zhang, Y., Wu, B."Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro". Molecular Medicine Reports 18, no. 5 (2018): 4477-4485. https://doi.org/10.3892/mmr.2018.9472
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