Acute stimulatory effect of tumor necrosis factor on the basolateral 50 pS K channels in the thick ascending limb of the rat kidney

  • Authors:
    • Guoyan Zhang
    • Shiliang Gui
    • Weiqun Wang
    • Dexin Meng
    • Qingmin Meng
    • Haiyan Luan
    • Rixin Zhao
    • Jiatian Zhang
    • Hongyu Sui
  • View Affiliations

  • Published online on: September 10, 2018     https://doi.org/10.3892/mmr.2018.9475
  • Pages: 4733-4738
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Abstract

The aim of the present study was to investigate the acute effect and mechanism of tumor necrosis factor (TNF) on basolateral 50 pS K channels in the thick ascending limb (TAL) of the rat kidney. The TAL tubules were isolated from the rat kidney, and the activity of the 50 pS K channels was recorded using the patch‑clamp technique. The results indicated that the application of TNF (10 nM) significantly activated the 50 pS K channels and the TNF effect was concentration‑dependent. Inhibition of protein kinase A, phospholipase A2 and protein tyrosine kinase using pathway inhibitors (H89, AACOCF3 and Herbimycin A, respectively) did not abolish the stimulatory effect of TNF, indicating that none of these pathways mediated the TNF effect. By contrast, the phenylarsine oxide inhibitor against protein tyrosine phosphatase (PTP) decreased the activity of the 50 pS K channels and blocked the stimulatory effect of TNF on these channels. Furthermore, western blot analysis demonstrated that the application of TNF (10 nM) in the TAL increased the phosphorylation of PTP, an indication of PTP activity stimulation. Thus, it was concluded that the acute application of TNF may stimulate the basolateral 50 pS K channel in the TAL and the stimulatory effect of TNF may be mediated by the PTP‑dependent pathway.
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November-2018
Volume 18 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang G, Gui S, Wang W, Meng D, Meng Q, Luan H, Zhao R, Zhang J and Sui H: Acute stimulatory effect of tumor necrosis factor on the basolateral 50 pS K channels in the thick ascending limb of the rat kidney. Mol Med Rep 18: 4733-4738, 2018
APA
Zhang, G., Gui, S., Wang, W., Meng, D., Meng, Q., Luan, H. ... Sui, H. (2018). Acute stimulatory effect of tumor necrosis factor on the basolateral 50 pS K channels in the thick ascending limb of the rat kidney. Molecular Medicine Reports, 18, 4733-4738. https://doi.org/10.3892/mmr.2018.9475
MLA
Zhang, G., Gui, S., Wang, W., Meng, D., Meng, Q., Luan, H., Zhao, R., Zhang, J., Sui, H."Acute stimulatory effect of tumor necrosis factor on the basolateral 50 pS K channels in the thick ascending limb of the rat kidney". Molecular Medicine Reports 18.5 (2018): 4733-4738.
Chicago
Zhang, G., Gui, S., Wang, W., Meng, D., Meng, Q., Luan, H., Zhao, R., Zhang, J., Sui, H."Acute stimulatory effect of tumor necrosis factor on the basolateral 50 pS K channels in the thick ascending limb of the rat kidney". Molecular Medicine Reports 18, no. 5 (2018): 4733-4738. https://doi.org/10.3892/mmr.2018.9475