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Article

Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation

  • Authors:
    • Junko Ikemoto
    • Satoshi Yoshihara
    • Tamami Kobayashi
    • Shunro Kai
    • Yoshihiro Fujimori
  • View Affiliations / Copyright

    Affiliations: Center for Blood Transfusion and Cellular Therapy, Hyogo College of Medicine Hospital, Nishinomiya, Hyogo 663‑8501, Japan
  • Pages: 4530-4534
    |
    Published online on: September 13, 2018
       https://doi.org/10.3892/mmr.2018.9481
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Abstract

In umbilical cord blood transplantation (UCBT), the number of cluster of differentiation (CD)34+ cells and colony‑forming units (CFUs) in the cord blood (CB) graft positively correlate with patient survival. Therefore, these parameters are currently used for quality assessment of the cryopreserved CB cells in the attached segment that is considered representative of the CB in the main bag prior to UCBT. Since aldehyde dehydrogenase (ALDH) activity is high in hematopoietic stem cells, the number of ALDH‑bright (ALDHbr) cells was examined in comparison with the number of CD34+ cells and CFUs for the quality assessment of CB units. In the cryopreserved main bag, the number of ALDHbr cells in the CB unit exhibited positive correlation with the number of CD34+ cells, and with CFU‑granulocytes/macrophages and total CFU counts. Furthermore, the concentration of ALDHbr cells in the cryopreserved attached segment was not significantly different compared to that of the main bag, suggesting that the attached segment is representative of the main bag. In conclusion, the present study suggested that ALDHbr cell counts in the cryopreserved attached segments may serve as a quality assessment indicator for CB units prior to UCBT.
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Copy and paste a formatted citation
Spandidos Publications style
Ikemoto J, Yoshihara S, Kobayashi T, Kai S and Fujimori Y: Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation. Mol Med Rep 18: 4530-4534, 2018.
APA
Ikemoto, J., Yoshihara, S., Kobayashi, T., Kai, S., & Fujimori, Y. (2018). Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation. Molecular Medicine Reports, 18, 4530-4534. https://doi.org/10.3892/mmr.2018.9481
MLA
Ikemoto, J., Yoshihara, S., Kobayashi, T., Kai, S., Fujimori, Y."Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation". Molecular Medicine Reports 18.5 (2018): 4530-4534.
Chicago
Ikemoto, J., Yoshihara, S., Kobayashi, T., Kai, S., Fujimori, Y."Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation". Molecular Medicine Reports 18, no. 5 (2018): 4530-4534. https://doi.org/10.3892/mmr.2018.9481
Copy and paste a formatted citation
x
Spandidos Publications style
Ikemoto J, Yoshihara S, Kobayashi T, Kai S and Fujimori Y: Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation. Mol Med Rep 18: 4530-4534, 2018.
APA
Ikemoto, J., Yoshihara, S., Kobayashi, T., Kai, S., & Fujimori, Y. (2018). Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation. Molecular Medicine Reports, 18, 4530-4534. https://doi.org/10.3892/mmr.2018.9481
MLA
Ikemoto, J., Yoshihara, S., Kobayashi, T., Kai, S., Fujimori, Y."Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation". Molecular Medicine Reports 18.5 (2018): 4530-4534.
Chicago
Ikemoto, J., Yoshihara, S., Kobayashi, T., Kai, S., Fujimori, Y."Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation". Molecular Medicine Reports 18, no. 5 (2018): 4530-4534. https://doi.org/10.3892/mmr.2018.9481
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