Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia‑reperfusion

Wang,Bing; Xu,Hua; Li,Jian; Gao,Hong‑Mei; Xing,Ying‑Hong; Lin,Zhu; Li,Hong‑Jie; Wang,Yong‑Qiang; Cao,Shu‑Hua

doi:10.3892/mmr.2018.9484

November-2018 Volume 18 Issue 5

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November-2018 Volume 18 Issue 5

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Article Open Access

Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia‑reperfusion

Authors:
- Bing Wang
- Hua Xu
- Jian Li
- Hong‑Mei Gao
- Ying‑Hong Xing
- Zhu Lin
- Hong‑Jie Li
- Yong‑Qiang Wang
- Shu‑Hua Cao
View Affiliations / Copyright

Affiliations: Department of Intensive Care Unit and Key Laboratory for Critical Care Medicine of the Ministry of Health, Emergency Medicine Research Institute, Tianjin First Center Hospital, Tianjin 300192, P.R. China

Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 4523-4529
|
Published online on: September 14, 2018

https://doi.org/10.3892/mmr.2018.9484
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Abstract

Increasing evidence has demonstrated that complement activation is required for ischemia‑reperfusion injury (IRI)‑induced hepatic damage, and cobra venom factor (CVF) can deplete the complement components. The aim of the current study was to investigate the effect and intrinsic mechanism of CVF pretreatment on IRI‑induced acute hepatic injury in rats. Acute hepatic injury in rats was induced by bone fracture to simulate trauma, followed by hemorrhage for 90 min, and then the rats were resuscitated for a period of 20 min of reperfusion. The survival times under different CVF treatment doses and schedules for rats with IRI were evaluated. Hepatic tissues and serum samples were analyzed for acute hepatic injury, complement activation, inflammatory mediator release and apoptosis at predetermined times and compared between the IRI group and the CVF pretreatment + IRI groups. Compared to the rats with IRI alone, the survival times were significantly improved among rats with IRI receiving a high‑dose or low‑dose CVF pretreatment (all P<0.01). Upon histological examination, severe hepatic damage was observed in the rats with IRI, accompanied by liver function deterioration, complement and membrane attack complex activation, inflammatory mediator release and hepatic cell apoptosis. CVF pretreatment significantly attenuated the hepatic injury through depletion of anaphylatoxic C5a and membrane attack complex C5b‑9 activation, and subsequent inhibition of inflammatory mediator release and hepatic cell apoptosis (all P<0.05). The results indicated that CVF pretreatment ameliorates IRI‑induced acute hepatic injury. However, further studies are required to determine whether this therapy could be a potential agent for the treatment of IRI injuries in clinical settings.

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1	Cannistrà M, Ruggiero M, Zullo A, Gallelli G, Serafini S, Maria M, Naso A, Grande R, Serra R and Nardo B: Hepatic ischemia reperfusion injury: A systematic review of literature and the role of current drugs and biomarkers. Int J Surg. 33 Suppl 1:S57–S70. 2016. View Article : Google Scholar : PubMed/NCBI
2	Peralta C, Jiménez-Castro MB and Gracia-Sancho J: Hepatic ischemia and reperfusion injury: Effects on the liver sinusoidal milieu. J Hepatol. 59:1094–1106. 2013. View Article : Google Scholar : PubMed/NCBI
3	Abe Y, Hines IN, Zibari G, Pavlick K, Gray L, Kitagawa Y and Grisham MB: Mouse model of liver ischemia and reperfusion injury: Method for studying reactive oxygen and nitrogen metabolites in vivo. Free Radic Biol Med. 46:1–7. 2009. View Article : Google Scholar : PubMed/NCBI
4	Pantazi E, Bejaoui M, Folch-Puy E, Adam R and Roselló-Catafau J: Advances in treatment strategies for ischemia reperfusion injury. Expert Opin Pharmacother. 17:169–179. 2016. View Article : Google Scholar : PubMed/NCBI
5	Gorsuch WB, Chrysanthou E, Schwaeble WJ and Stahl GL: The complement system in ischemia-reperfusion injuries. Immunobiology. 217:1026–1033. 2012. View Article : Google Scholar : PubMed/NCBI
6	Bajic G, Degn SE, Thiel S and Andersen GR: Complement activation, regulation, and molecular basis for complement-related diseases. EMBO J. 34:2735–2757. 2015. View Article : Google Scholar : PubMed/NCBI
7	Melis JP, Strumane K, Ruuls SR, Beurskens FJ, Schuurman J and Parren PW: Complement in therapy and disease: Regulating the complement system with antibody-based therapeutics. Mol Immunol. 67:117–130. 2015. View Article : Google Scholar : PubMed/NCBI
8	Krishnan V, Ponnuraj K, Xu Y, Macon K, Volanakis JE and Narayana SV: The crystal structure of cobra venom factor, a cofactor for C3- and C5-convertase CVFBb. Structure. 17:611–619. 2009. View Article : Google Scholar : PubMed/NCBI
9	Vogel CW and Fritzinger DC: Cobra venom factor: Structure, function, and humanization for therapeutic complement depletion. Toxicon. 56:1198–1222. 2010. View Article : Google Scholar : PubMed/NCBI
10	Vogel CW, Finnegan PW and Fritzinger DC: Humanized cobra venom factor: Structure, activity, and therapeutic efficacy in preclinical disease models. Mol Immunol. 61:191–203. 2014. View Article : Google Scholar : PubMed/NCBI
11	Mao YF, Yu QH, Zheng XF, Liu K, Liang WQ, Wang YW, Deng XW and Jiang L: Pre-treatment with cobra venom factor alleviates acute lung injury induced by intestinal ischemia-reperfusion in rats. Eur Rev Med Pharmacol Sci. 17:2207–2217. 2013.PubMed/NCBI
12	Gorsuch WB, Guikema BJ, Fritzinger DC, Vogel CW and Stahl GL: Humanized cobra venom factor decreases myocardial ischemia-reperfusion injury. Mol Immunol. 47:506–510. 2009. View Article : Google Scholar : PubMed/NCBI
13	Figueroa E, Gordon LE, Feldhoff PW and Lassiter HA: The administration of cobra venom factor reduces post-ischemic cerebra injury in adult and neonatal rats. Neurosci Lett. 380:48–53. 2005. View Article : Google Scholar : PubMed/NCBI
14	Vogel CW, Fritzinger DC, Gorsuch WB and Stahl GL: Complement depletion with humanized cobra venom factor: Efficacy in preclinical models of vascular disease. Thromb Haemost. 113:548–552. 2015. View Article : Google Scholar : PubMed/NCBI
15	Sun QY, Chen G, Guo H, Chen S, Wang WY and Xiong YL: Prolonged cardiac xenograft survival in guinea pig-to-rat model by a highly active cobra venom factor. Toxicon. 42:257–262. 2003. View Article : Google Scholar : PubMed/NCBI
16	Cui YL, Qiu LH, Zhou SY, Li LF, Qian ZZ, Liu XM, Zhang HL, Ren XB and Wang YQ: Necroptosis as a potential therapeutic target in multiple organ dysfunction syndrome. Oncotarget. 8:56980–56990. 2017. View Article : Google Scholar : PubMed/NCBI
17	Silverman J and Muir WW III: A review of laboratory animal anesthesia with chloral hydrate and chloralose. Lab Anim Sci. 43:210–216. 1993.PubMed/NCBI
18	Zimpfer M, Silt SP and Vatner SF: Effect of anesthesia on the canine carotid chemoreceptor reflex. Circ Res. 48:400–406. 1981. View Article : Google Scholar : PubMed/NCBI
19	Bahde R and Spiegel HU: Hepatic ischemia-reperfusion injury from bench to beside. Br J Surg. 97:1461–1475. 2010. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia‑reperfusion

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