Mitochondrial tRNAIle A4317G mutation may be associated with hearing impairment in a Han Chinese family

  • Authors:
    • Yong Cui
    • Duan‑Jun He
  • View Affiliations

  • Published online on: September 27, 2018     https://doi.org/10.3892/mmr.2018.9519
  • Pages: 5159-5165
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Abstract

Mutations in the mitochondrial genome have been identified to be associated with hearing loss. The aim of the present study was to investigate the role of mitochondrial DNA (mtDNA) variants in a Chinese family with hearing loss. Polymerase chain reaction (PCR)‑Sanger sequencing was used to screen the mtDNA variants and nuclear genes [gap junction protein β2 (GJB2) and transfer (t)RNA 5‑methylaminomethyle‑2‑thiouridylate methyltransferase (TRMU)]; in addition, the mtDNA copy number was determined by quantitative PCR. The present study characterized the molecular features of a Chinese family with maternally‑inherited hearing loss and identified mtDNA A1555G and tRNAIle A4317G mutations. The A4317G mutation was localized at the TΨC arm of tRNAIle (position 59) and created a novel base‑pairing (G59‑C54), which may alter the secondary structure of the tRNA. In addition, patients carrying the A4317G mutation exhibited a lower mtDNA copy number compared with the controls, suggesting that this mutation may cause mitochondrial dysfunction that is responsible for the deafness. However, no functional variants in the GJB2 and TRMU genes were detected. mtDNA A1555G and A4317G mutations may contribute to the clinical manifestation of hearing loss in this family.
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December-2018
Volume 18 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Cui Y and Cui Y: Mitochondrial tRNAIle A4317G mutation may be associated with hearing impairment in a Han Chinese family. Mol Med Rep 18: 5159-5165, 2018
APA
Cui, Y., & Cui, Y. (2018). Mitochondrial tRNAIle A4317G mutation may be associated with hearing impairment in a Han Chinese family. Molecular Medicine Reports, 18, 5159-5165. https://doi.org/10.3892/mmr.2018.9519
MLA
Cui, Y., He, D."Mitochondrial tRNAIle A4317G mutation may be associated with hearing impairment in a Han Chinese family". Molecular Medicine Reports 18.6 (2018): 5159-5165.
Chicago
Cui, Y., He, D."Mitochondrial tRNAIle A4317G mutation may be associated with hearing impairment in a Han Chinese family". Molecular Medicine Reports 18, no. 6 (2018): 5159-5165. https://doi.org/10.3892/mmr.2018.9519