Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3

  • Authors:
    • Young‑Tae Ro
    • Guk‑Heui Jo
    • Sun‑Ah Jung
    • Eunjoo H. Lee
    • Jongdae Shin
    • Joon H. Lee
  • View Affiliations

  • Published online on: October 1, 2018     https://doi.org/10.3892/mmr.2018.9525
  • Pages: 5133-5140
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Abstract

Salmonella enterica serovar Typhimurium (hereafter referred to as Salmonella), a virulent pathogen, is known to induce host‑cell death. Using reverse transcription‑quantitative polymerase chain reaction, a 28‑fold increase of microRNA (miR)‑155 expression in RAW 264.7 macrophages was observed following infection with Salmonella for 24 h. This miR‑155 upregulation increased macrophage cell death by up to 40% in 48 h following infection. Western blot analysis revealed that receptor interacting protein 1 (RIP1) and 3 (RIP3) were increased at 18 h following miR‑155 transfection to macrophages, similar to Salmonella infection. In addition, inhibition of RIP1 by pre‑incubating macrophages with necrostatin‑1, a RIP1 specific inhibitor, increased the viability of Salmonella‑infected cells and miR‑155‑transfected cells by up to 20%. The cleavage of poly (adenosine diphosphate‑ribose) polymerase‑1 (PARP‑1) was also enhanced by miR‑155 induction upon Salmonella infection. Therefore, it was suggested that RIP1/3‑induced necroptosis and PARP‑1‑mediated necrosis caused by miR‑155 induction may represent distinct routes of programmed necrotic cell death of Salmonella‑infected macrophages.

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Copy and paste a formatted citation
APA
Ro, Y., Jo, G., Jung, S., Lee, E.H., Shin, J., & Lee, J.H. (2018). Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3. Molecular Medicine Reports, 18, 5133-5140. https://doi.org/10.3892/mmr.2018.9525
MLA
Ro, Y., Jo, G., Jung, S., Lee, E. H., Shin, J., Lee, J. H."Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3". Molecular Medicine Reports 18.6 (2018): 5133-5140.
Chicago
Ro, Y., Jo, G., Jung, S., Lee, E. H., Shin, J., Lee, J. H."Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3". Molecular Medicine Reports 18, no. 6 (2018): 5133-5140. https://doi.org/10.3892/mmr.2018.9525