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Article

Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3

  • Authors:
    • Young‑Tae Ro
    • Guk‑Heui Jo
    • Sun‑Ah Jung
    • Eunjoo H. Lee
    • Jongdae Shin
    • Joon H. Lee
  • View Affiliations / Copyright

    Affiliations: Laboratory of Biochemistry, Graduate School of Medicine, Konkuk University, Chungju, Chungcheong 27478, Republic of Korea, Laboratory of Cell Biology, Myunggok Eye Research Institute, Konyang University College of Medicine, Seoul 07301, Republic of Korea, Graduate School of East‑West Medical Science, Kyung Hee University, Yongin, Gyeonggi 17104, Republic of Korea
  • Pages: 5133-5140
    |
    Published online on: October 1, 2018
       https://doi.org/10.3892/mmr.2018.9525
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Abstract

Salmonella enterica serovar Typhimurium (hereafter referred to as Salmonella), a virulent pathogen, is known to induce host‑cell death. Using reverse transcription‑quantitative polymerase chain reaction, a 28‑fold increase of microRNA (miR)‑155 expression in RAW 264.7 macrophages was observed following infection with Salmonella for 24 h. This miR‑155 upregulation increased macrophage cell death by up to 40% in 48 h following infection. Western blot analysis revealed that receptor interacting protein 1 (RIP1) and 3 (RIP3) were increased at 18 h following miR‑155 transfection to macrophages, similar to Salmonella infection. In addition, inhibition of RIP1 by pre‑incubating macrophages with necrostatin‑1, a RIP1 specific inhibitor, increased the viability of Salmonella‑infected cells and miR‑155‑transfected cells by up to 20%. The cleavage of poly (adenosine diphosphate‑ribose) polymerase‑1 (PARP‑1) was also enhanced by miR‑155 induction upon Salmonella infection. Therefore, it was suggested that RIP1/3‑induced necroptosis and PARP‑1‑mediated necrosis caused by miR‑155 induction may represent distinct routes of programmed necrotic cell death of Salmonella‑infected macrophages.
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Copy and paste a formatted citation
Spandidos Publications style
Ro YT, Jo GH, Jung SA, Lee EH, Shin J and Lee JH: Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3. Mol Med Rep 18: 5133-5140, 2018.
APA
Ro, Y., Jo, G., Jung, S., Lee, E.H., Shin, J., & Lee, J.H. (2018). Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3. Molecular Medicine Reports, 18, 5133-5140. https://doi.org/10.3892/mmr.2018.9525
MLA
Ro, Y., Jo, G., Jung, S., Lee, E. H., Shin, J., Lee, J. H."Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3". Molecular Medicine Reports 18.6 (2018): 5133-5140.
Chicago
Ro, Y., Jo, G., Jung, S., Lee, E. H., Shin, J., Lee, J. H."Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3". Molecular Medicine Reports 18, no. 6 (2018): 5133-5140. https://doi.org/10.3892/mmr.2018.9525
Copy and paste a formatted citation
x
Spandidos Publications style
Ro YT, Jo GH, Jung SA, Lee EH, Shin J and Lee JH: Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3. Mol Med Rep 18: 5133-5140, 2018.
APA
Ro, Y., Jo, G., Jung, S., Lee, E.H., Shin, J., & Lee, J.H. (2018). Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3. Molecular Medicine Reports, 18, 5133-5140. https://doi.org/10.3892/mmr.2018.9525
MLA
Ro, Y., Jo, G., Jung, S., Lee, E. H., Shin, J., Lee, J. H."Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3". Molecular Medicine Reports 18.6 (2018): 5133-5140.
Chicago
Ro, Y., Jo, G., Jung, S., Lee, E. H., Shin, J., Lee, J. H."Salmonella‑induced miR‑155 enhances necroptotic death in macrophage cells via targeting RIP1/3". Molecular Medicine Reports 18, no. 6 (2018): 5133-5140. https://doi.org/10.3892/mmr.2018.9525
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