Formononetin may protect aged hearts from ischemia/reperfusion damage by enhancing autophagic degradation

Huang,Zhengxin; Liu,Yingfeng; Huang,Xianping

doi:10.3892/mmr.2018.9544

Formononetin may protect aged hearts from ischemia/reperfusion damage by enhancing autophagic degradation

Authors:
- Zhengxin Huang
- Yingfeng Liu
- Xianping Huang
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Affiliations: Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510000, P.R. China, Laboratory of Biochemistry, Hunan University of Chinese Medicine, Changsha, Hunan 410000, P.R. China
Published online on: October 10, 2018 https://doi.org/10.3892/mmr.2018.9544
Pages: 4821-4830
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Myocardial infarction is a leading cause of mortality worldwide, and timely blood/oxygen reperfusion may substantially improve the outcome of infarction. However, ischemia/reperfusion (I/R) may cause severe side effects through excess reactive oxygen species generation. To develop novel methods to relieve I/R induced cell damage, the present study used a component of traditional Chinese medicine. In the present study, isolated heart tissue from aged mice and H9C2 cells with chemically‑induced aging were used as experimental subjects, and it was demonstrated that formononetin was able to alleviate I/R‑induced cell or tissue apoptosis. By applying formononetin to I/R‑damaged tissue or cells, it was demonstrated that formononetin was able to enhance autophagy and thus alleviate I/R‑induced cell damage. Furthermore, it was observed that I/R was able to inhibit lysosomal degradation processes in aged tissues or cells by impairing the lysosome acidification level, and formononetin was able to reverse this process via the re‑acidification of lysosomes. In conclusion, the present study demonstrated that formononetin was able to alleviate I/R‑induced cellular apoptosis in aged cells by facilitating autophagy.

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