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Article

Role of JNK signalling pathway and platelet‑lymphocyte aggregates in myocardial ischemia‑reperfusion injury and the cardioprotective effect of ischemic postconditioning in rats

  • Authors:
    • Faxin Ren
    • Nan Mu
    • Mingxiao Gao
    • Jing Sun
    • Chuanhuan Zhang
    • Xiaojian Sun
    • Liudong Li
    • Jun Li
    • Tong Liu
    • Gary Tse
    • Mei Dong
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Gynaecology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Tianjin Key Laboratory of Ionic‑Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China, Li Ka Shing Institute of Health Sciences, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong, SAR, P.R. China
  • Pages: 5237-5242
    |
    Published online on: October 10, 2018
       https://doi.org/10.3892/mmr.2018.9545
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Abstract

In myocardial ischemia‑reperfusion injury (MIRI), increased activity of the c‑Jun N‑terminal kinase (JNK) pathway and the activation of platelets that leads to the formation of platelet‑leukocyte aggregates (PLAs) have been observed. It was hypothesized that ischemic postconditioning in MIRI exerts cardioprotective effects by altering JNK activity, which in turn leads to reduced PLA levels. A total of 60 rats were randomly divided into 6 groups (n=10 for each group): i) Control; ii) ischemia‑reperfusion injury alone; iii) ischemia‑reperfusion with postconditioning (PostC group), iv) treatment with the JNK inhibitor‑SP600125; v) postC and treatment with anisomycin; and vi) treatment with the JNK activator‑anisomycin. Subsequently, the levels of PLA, infarct size, myocardial injury markers (creatinine kinase‑muscle/brain and troponin I) and were measured. Western blotting was used to determine the protein expression of phosphorylated‑JNK. MIRI led to increased myocardial infarct size that was associated with raised troponin I and creatine kinase‑muscle/brain. At different time points of MIRI, the level of PLA gradually increased. Compared with the injury‑reperfusion group, the level of PLA in the PostC and Inhibitor‑JNK groups was significantly reduced at 60 min and 3 h following reperfusion. MIRI was able to increase the expression of phosphorylated JNK. These effects were significantly reduced by ischemic postC or by treatment with SP600125. By contrast, the addition of anisomycin attenuated these protective effects. JNK is a critical mediator of MIRI. Ischemic postC can reduce the level of PLA during reperfusion by inhibiting the phosphorylation of JNK MAPK, thereby reducing MIRI. Pharmacological inhibition and activation of JNK can improve and reduce cardioprotective effects, respectively. These results explained the mechanism of the cardioprotection of postC and provided novel insight and target for the therapeutic strategy of MIRI.
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Copy and paste a formatted citation
Spandidos Publications style
Ren F, Mu N, Gao M, Sun J, Zhang C, Sun X, Li L, Li J, Liu T, Tse G, Tse G, et al: Role of JNK signalling pathway and platelet‑lymphocyte aggregates in myocardial ischemia‑reperfusion injury and the cardioprotective effect of ischemic postconditioning in rats. Mol Med Rep 18: 5237-5242, 2018.
APA
Ren, F., Mu, N., Gao, M., Sun, J., Zhang, C., Sun, X. ... Dong, M. (2018). Role of JNK signalling pathway and platelet‑lymphocyte aggregates in myocardial ischemia‑reperfusion injury and the cardioprotective effect of ischemic postconditioning in rats. Molecular Medicine Reports, 18, 5237-5242. https://doi.org/10.3892/mmr.2018.9545
MLA
Ren, F., Mu, N., Gao, M., Sun, J., Zhang, C., Sun, X., Li, L., Li, J., Liu, T., Tse, G., Dong, M."Role of JNK signalling pathway and platelet‑lymphocyte aggregates in myocardial ischemia‑reperfusion injury and the cardioprotective effect of ischemic postconditioning in rats". Molecular Medicine Reports 18.6 (2018): 5237-5242.
Chicago
Ren, F., Mu, N., Gao, M., Sun, J., Zhang, C., Sun, X., Li, L., Li, J., Liu, T., Tse, G., Dong, M."Role of JNK signalling pathway and platelet‑lymphocyte aggregates in myocardial ischemia‑reperfusion injury and the cardioprotective effect of ischemic postconditioning in rats". Molecular Medicine Reports 18, no. 6 (2018): 5237-5242. https://doi.org/10.3892/mmr.2018.9545
Copy and paste a formatted citation
x
Spandidos Publications style
Ren F, Mu N, Gao M, Sun J, Zhang C, Sun X, Li L, Li J, Liu T, Tse G, Tse G, et al: Role of JNK signalling pathway and platelet‑lymphocyte aggregates in myocardial ischemia‑reperfusion injury and the cardioprotective effect of ischemic postconditioning in rats. Mol Med Rep 18: 5237-5242, 2018.
APA
Ren, F., Mu, N., Gao, M., Sun, J., Zhang, C., Sun, X. ... Dong, M. (2018). Role of JNK signalling pathway and platelet‑lymphocyte aggregates in myocardial ischemia‑reperfusion injury and the cardioprotective effect of ischemic postconditioning in rats. Molecular Medicine Reports, 18, 5237-5242. https://doi.org/10.3892/mmr.2018.9545
MLA
Ren, F., Mu, N., Gao, M., Sun, J., Zhang, C., Sun, X., Li, L., Li, J., Liu, T., Tse, G., Dong, M."Role of JNK signalling pathway and platelet‑lymphocyte aggregates in myocardial ischemia‑reperfusion injury and the cardioprotective effect of ischemic postconditioning in rats". Molecular Medicine Reports 18.6 (2018): 5237-5242.
Chicago
Ren, F., Mu, N., Gao, M., Sun, J., Zhang, C., Sun, X., Li, L., Li, J., Liu, T., Tse, G., Dong, M."Role of JNK signalling pathway and platelet‑lymphocyte aggregates in myocardial ischemia‑reperfusion injury and the cardioprotective effect of ischemic postconditioning in rats". Molecular Medicine Reports 18, no. 6 (2018): 5237-5242. https://doi.org/10.3892/mmr.2018.9545
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