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Article

CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44

  • Authors:
    • Zhichao Fu
    • Pei Zhang
    • Huachun Luo
    • Huijuan Huang
    • Fengmei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Dongfang Hospital, Xiamen University, Fuzhou, Fujian 350025, P.R. China, Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, P.R. China, Department of Radiation Oncology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, Fujian 350025, P.R. China, Department of Gynaecology and Obstetrics, Dongfang Hospital, Xiamen University, Fuzhou, Fujian 350025, P.R. China
  • Pages: 5101-5108
    |
    Published online on: October 12, 2018
       https://doi.org/10.3892/mmr.2018.9554
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Abstract

The aim of the present study was to investigate the regulation of stromal cell‑derived factor 1 (CXCL12) in the radioresistance of cervical cancer, which was upregulated in tumors in our previous study. A CCK‑8 assay was used to detect cell viability. Flow cytometry was used to measure cell apoptosis and the expression levels of CD44 and CXCR4. ELISA was performed to measure the expression level of CXCL12 protein and CXCL12 mRNA was detected by reverse transcription‑quantitative polymerase chain reaction assays. Cell viability and apoptosis were determined with or without treatment with CXCL12 small interfering (si)RNA to examine the function of CXCL12 in Hela cells. The expression level of CD44 antigen (CD44) and C‑X‑C chemokine receptor type 4 (CXCR4) were measured using flow cytometry in the presence of CXCL12 and irradiation. In the present study, it was demonstrated that inhibition of CXCL12 reduced cell viability and increased cellular apoptosis in Hela cells treated with irradiation. Following treatment with CXCL12 siRNA, the expression level of CD44 was downregulated and the expression level of CXCR4 was upregulated. This effect of regulation additionally occurred in the presence of irradiation. In conclusion, the present data demonstrated that CXCL12 served an important role in the radioresistance of cervical cancer, suggestinh a novel therapeutic target.
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1 

Sun C, Reimers LL and Burk RD: Methylation of HPV16 genome CpG sites is associated with cervix precancer and cancer. Gynecol Oncol. 121:59–63. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Hu SY, Zheng RS, Zhao FH, Zhang SW, Chen WQ and Qiao YL: Trend analysis of cervical cancer incidence and mortality rates in Chinese women during 1989–1989. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 36:119–25. 2014.(In Chinese). PubMed/NCBI

3 

Jiang P, Liu J, Li W, Zeng X and Tang J: Role of p53 and p21 polymorphisms in the risk of cervical cancer among Chinese women. Acta Biochim Biophys Sin (Shanghai). 42:671–676. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Zhang B, Chen J, Ren Z, Chen Y, Li J, Miao X, Song Y, Zhao T, Li Y, Shi Y, et al: A specific miRNA signature promotes radioresistance of human cervical cancer cells. Cancer Cell Int. 13:1182013. View Article : Google Scholar : PubMed/NCBI

5 

Thompson RF and Maity A: Radiotherapy and the tumor microenvironment: Mutual influence and clinical implications. Adv Exp Med Biol. 772:147–165. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Shinohara ET and Maity A: Increasing sensitivity to radiotherapy and chemotherapy by using novel biological agents that alter the tumor microenvironment. Curr Mol Med. 9:1034–1045. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Fu ZC, Wang FM and Cai JM: Gene expression changes in residual advanced cervical cancer after radiotherapy: Indicators of poor prognosis and radioresistance? Med Sci Monit. 21:1276–1287. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Dubrovska A, Elliott J, Salamone RJ, Telegeev GD, Stakhovsky AE, Schepotin IB, Yan F, Wang Y, Bouchez LC, Kularatne SA, et al: CXCR4 expression in prostate cancer progenitor cells. PLoS One. 7:e312262012. View Article : Google Scholar : PubMed/NCBI

10 

Fuchs K, Hippe A, Schmaus A, Homey B, Sleeman JP and Orian-Rousseau V: Opposing effects of high- and low-molecular weight hyaluronan on CXCL12-induced CXCR4 signaling depend on CD44. Cell Death Dis. 4:e8192013. View Article : Google Scholar : PubMed/NCBI

11 

Goffart N, Lombard A, Lallemand F, Kroonen J, Nassen J, Di Valentin E, Berendsen S, Dedobbeleer M, Willems E, Robe P, et al: CXCL12 mediates glioblastoma resistance to radiotherapy in the subventricular zone. Neuro Oncol. 19:66–77. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Wang D, Jiao C, Zhu Y, Liang D, Zao M, Meng X, Gao J, He Y, Liu W, Hou J, et al: Activation of CXCL12/CXCR4 renders colorectal cancer cells less sensitive to radiotherapy via up-regulating the expression of survivin. Exp Biol Med (Maywood). 242:429–435. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Davis S, Lollo B, Freier S and Esau C: Improved targeting of miRNA with antisense oligonucleotides. Nucleic Acids Res. 34:2294–2304. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Barker HE, Paget JT, Khan AA and Harrington KJ: The tumour microenvironment after radiotherapy: Mechanisms of resistance and recurrence. Nat Rev Cancer. 15:409–425. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Zips D, Eicheler W, Brüchner K, Jackisch T, Geyer P, Petersen C, van der Kogel AJ and Baumann M: Impact of the tumour bed effect on microenvironment, radiobiological hypoxia and the outcome of fractionated radiotherapy of human FaDu squamous-cell carcinoma growing in the nude mouse. Int J Radiat Biol. 77:1185–1193. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Egeblad M and Werb Z: New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer. 2:161–174. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Tachibana K, Hirota S, Iizasa H, Yoshida H, Kawabata K, Kataoka Y, Kitamura Y, Matsushima K, Yoshida N, Nishikawa S, et al: The chemokine receptor CXCR4 is essential for vascularization of the gastrointestinal tract. Nature. 393:591–594. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Dorsam RT and Gutkind JS: G-protein-coupled receptors and cancer. Nat Rev Cancer. 7:79–94. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Takakura N, Watanabe T, Suenobu S, Yamada Y, Noda T, Ito Y, Satake M and Suda T: A role for hematopoietic stem cells in promoting angiogenesis. Cell. 102:199–209. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Park HJ, Griffin RJ, Hui S, Levitt SH and Song CW: Radiation-induced vascular damage in tumors: Implications of vascular damage in ablative hypofractionated radiotherapy (SBRT and SRS). Radiat Res. 177:311–327. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Boimel PJ, Smirnova T, Zhou ZN, Wyckoff J, Park H, Coniglio SJ, Qian BZ, Stanley ER, Cox D, Pollard JW, et al: Contribution of CXCL12 secretion to invasion of breast cancer cells. Breast Cancer Res. 14:R232012. View Article : Google Scholar : PubMed/NCBI

23 

Burns JM, Summers BC, Wang Y, Melikian A, Berahovich R, Miao Z, Penfold ME, Sunshine MJ, Littman DR, Kuo CJ, et al: A novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development. J Exp Med. 203:2201–2213. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Burger JA and Kipps TJ: CXCR4: A key receptor in the crosstalk between tumor cells and their microenvironment. Blood. 107:1761–1767. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Kucia M, Reca R, Miekus K, Wanzeck J, Wojakowski W, Janowska-Wieczorek A, Ratajczak J and Ratajczak MZ: Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: Pivotal role of the SDF-1-CXCR4 axis. Stem Cells. 23:879–894. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Zhang XH, Jin X, Malladi S, Zou Y, Wen YH, Brogi E, Smid M, Foekens JA and Massagué J: Selection of bone metastasis seeds by mesenchymal signals in the primary tumor stroma. Cell. 154:1060–1073. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Barbero S, Bonavia R, Bajetto A, Porcile C, Pirani P, Ravetti JL, Zona GL, Spaziante R, Florio T and Schettini G: Stromal cell-derived factor 1alpha stimulates human glioblastoma cell growth through the activation of both extracellular signal-regulated kinases 1/2 and Akt. Cancer Res. 63:1969–1974. 2003.PubMed/NCBI

28 

Avigdor A, Goichberg P, Shivtiel S, Dar A, Peled A, Samira S, Kollet O, Hershkoviz R, Alon R, Hardan I, et al: CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow. Blood. 103:2981–2989. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Huang Y, Zhang J, Cui ZM, Zhao J and Zheng Y: Expression of the CXCL12/CXCR4 and CXCL16/CXCR6 axes in cervical intraepithelial neoplasia and cervical cancer. Chin J Cancer. 32:289–296. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Faber A, Goessler UR, Hoermann K, Schultz JD, Umbreit C and Stern-Straeter J: SDF-1-CXCR4 axis: Cell trafficking in the cancer stem cell niche of head and neck squamous cell carcinoma. Oncol Rep. 29:2325–2331. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Müller A, Homey B, Soto H, Ge N, Catron D, Buchanan ME, McClanahan T, Murphy E, Yuan W, Wagner SN, et al: Involvement of chemokine receptors in breast cancer metastasis. Nature. 410:50–56. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fu Z, Zhang P, Luo H, Huang H and Wang F: CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44. Mol Med Rep 18: 5101-5108, 2018.
APA
Fu, Z., Zhang, P., Luo, H., Huang, H., & Wang, F. (2018). CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44. Molecular Medicine Reports, 18, 5101-5108. https://doi.org/10.3892/mmr.2018.9554
MLA
Fu, Z., Zhang, P., Luo, H., Huang, H., Wang, F."CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44". Molecular Medicine Reports 18.6 (2018): 5101-5108.
Chicago
Fu, Z., Zhang, P., Luo, H., Huang, H., Wang, F."CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44". Molecular Medicine Reports 18, no. 6 (2018): 5101-5108. https://doi.org/10.3892/mmr.2018.9554
Copy and paste a formatted citation
x
Spandidos Publications style
Fu Z, Zhang P, Luo H, Huang H and Wang F: CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44. Mol Med Rep 18: 5101-5108, 2018.
APA
Fu, Z., Zhang, P., Luo, H., Huang, H., & Wang, F. (2018). CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44. Molecular Medicine Reports, 18, 5101-5108. https://doi.org/10.3892/mmr.2018.9554
MLA
Fu, Z., Zhang, P., Luo, H., Huang, H., Wang, F."CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44". Molecular Medicine Reports 18.6 (2018): 5101-5108.
Chicago
Fu, Z., Zhang, P., Luo, H., Huang, H., Wang, F."CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44". Molecular Medicine Reports 18, no. 6 (2018): 5101-5108. https://doi.org/10.3892/mmr.2018.9554
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