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Article Open Access

Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats

  • Authors:
    • Pengcheng Xing
    • Ke Ma
    • Jun Wu
    • Wei Long
    • Donglian Wang
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Shanghai Sixth People's Hospital East Area Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai 201306, P.R. China, ICU, Shanghai Sixth People's Hospital East Area Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai 201306, P.R. China, Department of Geriatric Medicine, Shanghai Sixth People's Hospital East Area Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai 201306, P.R. China
    Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5371-5378
    |
    Published online on: October 23, 2018
       https://doi.org/10.3892/mmr.2018.9579
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Abstract

In the present study, the protective effects and regulatory mechanism of polysaccharide peptide (PSP) were investigated in rats with cerebral ischemia‑reperfusion (IR) injury. Neuroblastoma N2a cells were divided into five groups: Negative control; IR injury; PSP low dose treatment; PSP middle dose treatment; and PSP high dose treatment. In vitro, the cell viability was detected by an MTT assay. ELISA was performed to determine the activity of lactate dehydrogenase (LDH) and caspase‑3. A cerebral IR injury model in vivo was established, and hematoxylin and eosin (H&E) staining, western blotting, neurological deficit score and cerebral infarction were assessed. The cell viability was markedly improved following treatment with PSP and the activity of LDH and caspase‑3 was decreased following PSP administration (P<0.05). The in vivo studies determined that the neurological deficit score and cerebral infarction volume were reduced with the concentration of PSP increasing between 150 and 250 mg/kg. The H&E staining indicated that PSP was able to protect the nerve cells against the cerebral IR injury. In addition, PSP upregulated the decreased silent information regulator protein 1, peroxisome proliferator‑­activated receptor γ coactivator‑1α and apoptosis regulator B‑cell lymphoma 2 expression induced by cerebral IR injury. The protein expression level of caspase‑3 and apoptosis regulator apoptosis regulator Bcl‑2‑like protein 4 was downregulated following PSP administration. These results suggested that PSP may improve nerve cell viability, enhance the neuroprotective role in cerebral IR injury and provide a novel approach for the treatment of cerebral IR injury.
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Copy and paste a formatted citation
Spandidos Publications style
Xing P, Ma K, Wu J, Long W and Wang D: Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats. Mol Med Rep 18: 5371-5378, 2018.
APA
Xing, P., Ma, K., Wu, J., Long, W., & Wang, D. (2018). Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats. Molecular Medicine Reports, 18, 5371-5378. https://doi.org/10.3892/mmr.2018.9579
MLA
Xing, P., Ma, K., Wu, J., Long, W., Wang, D."Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats". Molecular Medicine Reports 18.6 (2018): 5371-5378.
Chicago
Xing, P., Ma, K., Wu, J., Long, W., Wang, D."Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats". Molecular Medicine Reports 18, no. 6 (2018): 5371-5378. https://doi.org/10.3892/mmr.2018.9579
Copy and paste a formatted citation
x
Spandidos Publications style
Xing P, Ma K, Wu J, Long W and Wang D: Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats. Mol Med Rep 18: 5371-5378, 2018.
APA
Xing, P., Ma, K., Wu, J., Long, W., & Wang, D. (2018). Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats. Molecular Medicine Reports, 18, 5371-5378. https://doi.org/10.3892/mmr.2018.9579
MLA
Xing, P., Ma, K., Wu, J., Long, W., Wang, D."Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats". Molecular Medicine Reports 18.6 (2018): 5371-5378.
Chicago
Xing, P., Ma, K., Wu, J., Long, W., Wang, D."Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats". Molecular Medicine Reports 18, no. 6 (2018): 5371-5378. https://doi.org/10.3892/mmr.2018.9579
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