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Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice

  • Authors:
    • Zhi Liu
    • Hong Chang
    • Yihong Hou
    • Yu Wang
    • Zhiqiang Zhou
    • Ming Wang
    • Zhidong Huang
    • Bin Yu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Surgery II, People's Hospital of Dongsheng, Ordos City, Inner Mongolia 017000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5317-5326
    |
    Published online on: October 25, 2018
       https://doi.org/10.3892/mmr.2018.9596
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Abstract

Repair of bone defects presents a serious clinical challenge as it is difficult to restore bone function and regenerate bone loss. In the present study, the effects of lentivirus‑mediated transfection of bone marrow mesenchymal stem cells (BMSCs) with microRNA (miR)‑26a on bone regeneration were investigated in a mouse bone defect repair model. Marker of proliferation Ki67 (Ki67) staining was employed to detect the cell proliferation capacity and alkaline phosphatase (ALP) staining was used to investigate osteogenic differentiation. A mouse model of cranial bone defects was established. β‑tricalcium phosphate biomaterials co‑cultured with the transfected BMSCs were implanted into the defect areas of mouse models. Micro‑computed tomography, and hematoxylin and eosin and toluidine blue staining, were used to detect bone regeneration in the defect areas and the degradation of scaffolds. miR‑26a expression, and the mRNA and protein expression of osteogenesis‑associated cytokines, were detected using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Separated and cultured BMSCs highly expressed CD29 and CD105, but not CD34 and CD45, as determined by flow cytometry. miR‑26a expression and the positive cell rate of Ki67 and ALP staining in BMSCs transfected with pLVTHM‑miR‑26a were increased. The BMSC and negative control‑transfected BMSC groups exhibited increased bone regeneration in the defect areas, increased bone volume of newly formed bones, and elevated mRNA and protein expression of runt‑related transcription factor 2 (Runx2) and osteocalcin (OC), compared with the blank group. However, the miR‑26a‑transfected BMSC group exhibited further increases in bone regeneration and the volume of newly formed bones, and further elevations of the mRNA and protein expression levels of Runx2 and OC. The present findings demonstrated that lentivirus‑mediated modification of BMSCs enhanced bone regeneration during the repair of cranial bone defects in mice.
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View References

1 

Kolambkar YM, Boerckel JD, Dupont KM, Bajin M, Huebsch N, Mooney DJ, Hutmacher DW and Guldberg RE: Spatiotemporal delivery of bone morphogenetic protein enhances functional repair of segmental bone defects. Bone. 49:485–492. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Calori GM, Mazza E, Colombo M and Ripamonti C: The use of bone-graft substitutes in large bone defects: Any specific needs? Injury. 42 Suppl 2:S56–S63. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Lv J, Xiu P, Tan J, Jia Z, Cai H and Liu Z: Enhanced angiogenesis and osteogenesis in critical bone defects by the controlled release of BMP-2 and VEGF: Implantation of electron beam melting-fabricated porous Ti6Al4V scaffolds incorporating growth factor-doped fibrin glue. Biomed Mater. 10:0350132015. View Article : Google Scholar : PubMed/NCBI

4 

Kusumbe AP, Ramasamy SK and Adams RH: Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature. 507:323–328. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Kolambkar YM, Dupont KM, Boerckel JD, Huebsch N, Mooney DJ, Hutmacher DW and Guldberg RE: An alginate-based hybrid system for growth factor delivery in the functional repair of large bone defects. Biomaterials. 32:65–74. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Li J, Hong J, Zheng Q, Guo X, Lan S, Cui F, Pan H, Zou Z and Chen C: Repair of rat cranial bone defects with nHAC/PLLA and BMP-2-related peptide or rhBMP-2. J Orthop Res. 29:1745–1752. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Zou D, Zhang Z, Ye D, Tang A, Deng L, Han W, Zhao J, Wang S, Zhang W, Zhu C, et al: Repair of critical-sized rat calvarial defects using genetically engineered bone marrow-derived mesenchymal stem cells overexpressing hypoxia-inducible factor-1α. Stem Cells. 29:1380–1390. 2011.PubMed/NCBI

8 

Zhang Y, Wang F, Chen J, Ning Z and Yang L: Bone marrow-derived mesenchymal stem cells versus bone marrow nucleated cells in the treatment of chondral defects. Int Orthop. 36:1079–1086. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Lin CY, Chang YH, Lin KJ, Yen TC, Tai CL, Chen CY, Lo WH, Hsiao IT and Hu YC: The healing of critical-sized femoral segmental bone defects in rabbits using baculovirus-engineered mesenchymal stem cells. Biomaterials. 31:3222–3230. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Liu JL, Jiang L, Lin QX, Deng CY, Mai LP, Zhu JN, Li XH, Yu XY, Lin SG and Shan ZX: MicroRNA 16 enhances differentiation of human bone marrow mesenchymal stem cells in a cardiac niche toward myogenic phenotypes in vitro. Life Sci. 90:1020–1026. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Chen S, Yang L, Jie Q, Lin YS, Meng GL, Fan JZ, Zhang JK, Fan J, Luo ZJ and Liu J: MicroRNA125b suppresses the proliferation and osteogenic differentiation of human bone marrowderived mesenchymal stem cells. Mol Med Rep. 9:1820–1826. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Xu JF, Yang GH, Pan XH, Zhang SJ, Zhao C, Qiu BS, Gu HF, Hong JF, Cao L, Chen Y, et al: Altered microRNA expression profile in exosomes during osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. PLoS One. 9:e1146272014. View Article : Google Scholar : PubMed/NCBI

13 

Zhang J, Han C and Wu T: MicroRNA-26a promotes cholangiocarcinoma growth by activating β-catenin. Gastroenterology. 143:246–256, e248. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Mohamed JS, Lopez MA and Boriek AM: Mechanical stretch up-regulates microRNA-26a and induces human airway smooth muscle hypertrophy by suppressing glycogen synthase kinase-3β. J Biol Chem. 285:29336–29347. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Yang X, Liang L, Zhang XF, Jia HL, Qin Y, Zhu XC, Gao XM, Qiao P, Zheng Y, Sheng YY, et al: MicroRNA-26a suppresses tumor growth and metastasis of human hepatocellular carcinoma by targeting interleukin-6-Stat3 pathway. Hepatology. 58:158–170. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Leeper NJ, Raiesdana A, Kojima Y, Chun HJ, Azuma J, Maegdefessel L, Kundu RK, Quertermous T, Tsao PS and Spin JM: MicroRNA-26a is a novel regulator of vascular smooth muscle cell function. J Cell Physiol. 226:1035–1043. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Bahi A, Chandrasekar V and Dreyer JL: Selective lentiviral-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like effects in rats. Psychoneuroendocrinology. 46:78–87. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Sun BS, Dong QZ, Ye QH, Sun HJ, Jia HL, Zhu XQ, Liu DY, Chen J, Xue Q, Zhou HJ, et al: Lentiviral-mediated miRNA against osteopontin suppresses tumor growth and metastasis of human hepatocellular carcinoma. Hepatology. 48:1834–1842. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Luzi E, Marini F, Tognarini I, Galli G, Falchetti A and Brandi ML: The regulatory network menin-microRNA 26a as a possible target for RNA-based therapy of bone diseases. Nucleic Acid Ther. 22:103–108. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Luzi E, Marini F, Sala SC, Tognarini I, Galli G and Brandi ML: Osteogenic differentiation of human adipose tissue-derived stem cells is modulated by the miR-26a targeting of the SMAD1 transcription factor. J Bone Miner Res. 23:287–295. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Ye JH, Xu YJ, Gao J, Yan SG, Zhao J, Tu Q, Zhang J, Duan XJ, Sommer CA, Mostoslavsky G, et al: Critical-size calvarial bone defects healing in a mouse model with silk scaffolds and SATB2-modified iPSCs. Biomaterials. 32:5065–5076. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Williams JR: The declaration of Helsinki and public health. Bull World Health Organ. 86:650–652. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Mikheev AG: 2 modifications of the calcium-cobalt method for cytochemical determination of alkaline phosphatase in leukocytes of the blood and bone marrow. Lab Delo. 12:711–713. 1969.(In Russian). PubMed/NCBI

24 

Li J, Jin L, Wang M, Zhu S and Xu S: Repair of rat cranial bone defect by using bone morphogenetic protein-2-related peptide combined with microspheres composed of polylactic acid/polyglycolic acid copolymer and chitosan. Biomed Mater. 10:0450042015. View Article : Google Scholar : PubMed/NCBI

25 

Xu L, Lv K, Zhang W, Zhang X, Jiang X and Zhang F: The healing of critical-size calvarial bone defects in rat with rhPDGF-BB, BMSCs, and β-TCP scaffolds. J Mater Sci Mater Med. 23:1073–1084. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Li TJ, Browne RM and Matthews JB: Epithelial cell proliferation in odontogenic keratocysts: A comparative immunocytochemical study of Ki67 in simple, recurrent and basal cell naevus syndrome (BCNS)-associated lesions. J Oral Pathol Med. 24:221–226. 1995. View Article : Google Scholar : PubMed/NCBI

28 

Giannoudis PV, Faour O, Goff T, Kanakaris N and Dimitriou R: Masquelet technique for the treatment of bone defects: Tips-tricks and future directions. Injury. 42:591–598. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Seeliger C, Karpinski K, Haug AT, Vester H, Schmitt A, Bauer JS and van Griensven M: Five freely circulating miRNAs and bone tissue miRNAs are associated with osteoporotic fractures. J Bone Miner Res. 29:1718–1728. 2014. View Article : Google Scholar : PubMed/NCBI

30 

van Wijnen AJ, van de Peppel J, van Leeuwen JP, Lian JB, Stein GS, Westendorf JJ, Oursler MJ, Im HJ, Taipaleenmäki H, Hesse E, et al: MicroRNA functions in osteogenesis and dysfunctions in osteoporosis. Curr Osteoporosis Rep. 11:72–82. 2013. View Article : Google Scholar

31 

Li KC, Chang YH, Yeh CL and Hu YC: Healing of osteoporotic bone defects by baculovirus-engineered bone marrow-derived MSCs expressing MicroRNA sponges. Biomaterials. 74:155–166. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Wang Z, Zhang D, Hu Z, Cheng J, Zhuo C, Fang X and Xing Y: MicroRNA-26a-modified adipose-derived stem cells incorporated with a porous hydroxyapatite scaffold improve the repair of bone defects. Mol Med Rep. 12:3345–3350. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Paquet J, Moya A, Bensidhoum M and Petite H: Engineered cell-free scaffold with two-stage delivery of miRNA-26a for bone repair. Ann Transl Med. 4:2042016. View Article : Google Scholar : PubMed/NCBI

34 

Li Y, Fan L, Liu S, Liu W, Zhang H, Zhou T, Wu D, Yang P, Shen L, Chen J and Jin Y: The promotion of bone regeneration through positive regulation of angiogenic-osteogenic coupling using microRNA-26a. Biomaterials. 34:5048–5058. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Jo HN, Kang H, Lee A, Choi J, Chang W, Lee MS and Kim J: Endothelial miR-26a regulates VEGF-Nogo-B receptor-mediated angiogenesis. BMB Rep. 50:384–389. 2017. View Article : Google Scholar : PubMed/NCBI

36 

Yuan J, Zhang WJ, Liu G, Wei M, Qi ZL, Liu W, Cui L and Cao YL: Repair of canine mandibular bone defects with bone marrow stromal cells and coral. Tissue Eng Part A. 16:1385–1394. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Tian H, Bharadwaj S, Liu Y, Ma PX, Atala A and Zhang Y: Differentiation of human bone marrow mesenchymal stem cells into bladder cells: Potential for urological tissue engineering. Tissue Eng Part A. 16:1769–1779. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Zhou J, Lin H, Fang T, Li X, Dai W, Uemura T and Dong J: The repair of large segmental bone defects in the rabbit with vascularized tissue engineered bone. Biomaterials. 31:1171–1179. 2010. View Article : Google Scholar : PubMed/NCBI

39 

Dupont KM, Sharma K, Stevens HY, Boerckel JD, Garcia AJ and Guldberg RE: Human stem cell delivery for treatment of large segmental bone defects. Proc Natl Acad Sci USA. 107:3305–3310. 2010. View Article : Google Scholar : PubMed/NCBI

40 

Hasegawa N, Kawaguchi H, Hirachi A, Takeda K, Mizuno N, Nishimura M, Koike C, Tsuji K, Iba H, Kato Y and Kurihara H: Behavior of transplanted bone marrow-derived mesenchymal stem cells in periodontal defects. J Periodontol. 77:1003–1007. 2006. View Article : Google Scholar : PubMed/NCBI

41 

Su X, Liao L, Shuai Y, Jing H, Liu S, Zhou H, Liu Y and Jin Y: MiR-26a functions oppositely in osteogenic differentiation of BMSCs and ADSCs depending on distinct activation and roles of Wnt and BMP signaling pathway. Cell Death Dis. 6:e18512015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Chang H, Hou Y, Wang Y, Zhou Z, Wang M, Huang Z and Yu B: Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice. Mol Med Rep 18: 5317-5326, 2018.
APA
Liu, Z., Chang, H., Hou, Y., Wang, Y., Zhou, Z., Wang, M. ... Yu, B. (2018). Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice. Molecular Medicine Reports, 18, 5317-5326. https://doi.org/10.3892/mmr.2018.9596
MLA
Liu, Z., Chang, H., Hou, Y., Wang, Y., Zhou, Z., Wang, M., Huang, Z., Yu, B."Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice". Molecular Medicine Reports 18.6 (2018): 5317-5326.
Chicago
Liu, Z., Chang, H., Hou, Y., Wang, Y., Zhou, Z., Wang, M., Huang, Z., Yu, B."Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice". Molecular Medicine Reports 18, no. 6 (2018): 5317-5326. https://doi.org/10.3892/mmr.2018.9596
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Chang H, Hou Y, Wang Y, Zhou Z, Wang M, Huang Z and Yu B: Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice. Mol Med Rep 18: 5317-5326, 2018.
APA
Liu, Z., Chang, H., Hou, Y., Wang, Y., Zhou, Z., Wang, M. ... Yu, B. (2018). Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice. Molecular Medicine Reports, 18, 5317-5326. https://doi.org/10.3892/mmr.2018.9596
MLA
Liu, Z., Chang, H., Hou, Y., Wang, Y., Zhou, Z., Wang, M., Huang, Z., Yu, B."Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice". Molecular Medicine Reports 18.6 (2018): 5317-5326.
Chicago
Liu, Z., Chang, H., Hou, Y., Wang, Y., Zhou, Z., Wang, M., Huang, Z., Yu, B."Lentivirus‑mediated microRNA‑26a overexpression in bone mesenchymal stem cells facilitates bone regeneration in bone defects of calvaria in mice". Molecular Medicine Reports 18, no. 6 (2018): 5317-5326. https://doi.org/10.3892/mmr.2018.9596
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