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Long‑term treatment with salicylate enables NMDA receptors and impairs AMPA receptors in C57BL/6J mice inner hair cell ribbon synapse

  • Authors:
    • Wanming Cui
    • Haolin Wang
    • Yu Cheng
    • Xiaorui Ma
    • Yu Lei
    • Xingxing Ruan
    • Lin Shi
    • Mei Lv
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116013, P.R. China
    Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 51-58
    |
    Published online on: November 5, 2018
       https://doi.org/10.3892/mmr.2018.9624
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Abstract

Salicylate is widely used to produce animal models of tinnitus in mice and/or rats. The side effects on auditory function, including hearing loss and tinnitus, are considered the results of the auditory nerve dysfunction. A recent study indicated that chronic treatment with salicylate for several weeks reduces compressed action potential amplitude, which is contradictory to the studies reporting excessive activation of N‑methyl‑D‑aspartate receptors (NMDAR) in tinnitus‑induced animals. The specific aims of the experiment were to detect the effect of salicylate on the inner hair cells (IHCs), ribbon synapse, as well as the association between the hearing threshold and the number of mismatched ribbon synapses. In the present study, mice were injected intraperitoneally with a low dose of salicylate (200 mg/kg) for 14 days. The auditory brainstem response and otoacoustic emission were measured to assess auditory function of the mice. The postsynaptic regions of IHC were identified with two types of immunostaining targets: Postsynaptic density protein 95 and Glu2/3. The number of spheres was counted and the synapses were reconstructed in 3‑dimensional images. Increases in distortion product otoacoustic emissions amplitudes of the salicylate group were detected, however, an elevation in the hearing threshold was also observed. A mismatch between pre‑and post‑ribbon synapses was observed. In addition, the cochlear components, including the numbers of outer hair cells and IHCs, were unlikely to be affected by salicylate. IHC ribbon synapses were more susceptible to salicylate stimuli. Furthermore, mismatch of pre‑ and post‑ribbon synapses may indicate a competitive inhibition between NMDAR and α-amino‑3‑hydroxy-5-methyl-4-isoxa-zole-propionate receptors and dysfunction of ribbon synapses.
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Copy and paste a formatted citation
Spandidos Publications style
Cui W, Wang H, Cheng Y, Ma X, Lei Y, Ruan X, Shi L and Lv M: Long‑term treatment with salicylate enables NMDA receptors and impairs AMPA receptors in C57BL/6J mice inner hair cell ribbon synapse. Mol Med Rep 19: 51-58, 2019.
APA
Cui, W., Wang, H., Cheng, Y., Ma, X., Lei, Y., Ruan, X. ... Lv, M. (2019). Long‑term treatment with salicylate enables NMDA receptors and impairs AMPA receptors in C57BL/6J mice inner hair cell ribbon synapse. Molecular Medicine Reports, 19, 51-58. https://doi.org/10.3892/mmr.2018.9624
MLA
Cui, W., Wang, H., Cheng, Y., Ma, X., Lei, Y., Ruan, X., Shi, L., Lv, M."Long‑term treatment with salicylate enables NMDA receptors and impairs AMPA receptors in C57BL/6J mice inner hair cell ribbon synapse". Molecular Medicine Reports 19.1 (2019): 51-58.
Chicago
Cui, W., Wang, H., Cheng, Y., Ma, X., Lei, Y., Ruan, X., Shi, L., Lv, M."Long‑term treatment with salicylate enables NMDA receptors and impairs AMPA receptors in C57BL/6J mice inner hair cell ribbon synapse". Molecular Medicine Reports 19, no. 1 (2019): 51-58. https://doi.org/10.3892/mmr.2018.9624
Copy and paste a formatted citation
x
Spandidos Publications style
Cui W, Wang H, Cheng Y, Ma X, Lei Y, Ruan X, Shi L and Lv M: Long‑term treatment with salicylate enables NMDA receptors and impairs AMPA receptors in C57BL/6J mice inner hair cell ribbon synapse. Mol Med Rep 19: 51-58, 2019.
APA
Cui, W., Wang, H., Cheng, Y., Ma, X., Lei, Y., Ruan, X. ... Lv, M. (2019). Long‑term treatment with salicylate enables NMDA receptors and impairs AMPA receptors in C57BL/6J mice inner hair cell ribbon synapse. Molecular Medicine Reports, 19, 51-58. https://doi.org/10.3892/mmr.2018.9624
MLA
Cui, W., Wang, H., Cheng, Y., Ma, X., Lei, Y., Ruan, X., Shi, L., Lv, M."Long‑term treatment with salicylate enables NMDA receptors and impairs AMPA receptors in C57BL/6J mice inner hair cell ribbon synapse". Molecular Medicine Reports 19.1 (2019): 51-58.
Chicago
Cui, W., Wang, H., Cheng, Y., Ma, X., Lei, Y., Ruan, X., Shi, L., Lv, M."Long‑term treatment with salicylate enables NMDA receptors and impairs AMPA receptors in C57BL/6J mice inner hair cell ribbon synapse". Molecular Medicine Reports 19, no. 1 (2019): 51-58. https://doi.org/10.3892/mmr.2018.9624
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