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Hydrogen‑rich medium alleviates high glucose‑induced oxidative stress and parthanatos in rat Schwann cells in vitro

  • Authors:
    • Qing Li
    • Yang Jiao
    • Yang Yu
    • Guolin Wang
    • Yonghao Yu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 338-344
    |
    Published online on: November 8, 2018
       https://doi.org/10.3892/mmr.2018.9631
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Abstract

Diabetic peripheral neuropathy (DPN) is considered to be the most common cause of microvascular diabetic complications, for which no effective therapies currently exist. Previous studies have identified that oxidative stress is the common pathway in all possible hypotheses for the induction of DPN, and poly(ADP‑ribose) (PAR) polymerase‑1 (PARP‑1)‑dependent cell death (parthanatos) is key in the pathogenic mechanisms of neurodegenerative disease. The aim of the present study was to investigate the protective effects and corresponding mechanisms of hydrogen‑rich medium (HM) on high glucose (HG)‑induced oxidative stress and parthanatos in primary rat Schwann cells (RSCs) in vitro. The RSCs were divided into groups and treated for 48 h. Cell counting kit‑8 and lactate dehydrogenase assays were used to detect cell viability and cytotoxicity, respectively; intracellular OH‑ levels were measured using a DCFH‑DA assay; concentrations of peroxynitrite (ONOO‑) and 8‑hydroxy deoxyguanosine (8‑OHdG) were evaluated with an enzyme‑linked immunosorbent assay; relative expression levels of parthanatos‑related proteins [PAR, nucleus apoptosis‑inducing factor (AIF) and total AIF] were analyzed using western blot analysis, and immunofluorescence was used to determine the nuclear translocation of AIF. After 48 h, HG was shown to induce severe oxidative stress and promote marked levels of parthanatos in the RSCs. Treatment with HM inhibited HG‑induced oxidative stress by reducing the production of OH‑ and ONOO‑ and suppressed parthanatos by downregulating the levels of 8‑OHdG, the expression of PAR and the nuclear translocation of AIF. HM improved cell viability and inhibited cytotoxicity under the HG condition. These results indicate that HM effectively reduces HG‑induced oxidative stress in RSCs and protects them against parthanatos. Therefore, HM may be a novel treatment for DPN.
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Copy and paste a formatted citation
Spandidos Publications style
Li Q, Jiao Y, Yu Y, Wang G and Yu Y: Hydrogen‑rich medium alleviates high glucose‑induced oxidative stress and parthanatos in rat Schwann cells in vitro. Mol Med Rep 19: 338-344, 2019.
APA
Li, Q., Jiao, Y., Yu, Y., Wang, G., & Yu, Y. (2019). Hydrogen‑rich medium alleviates high glucose‑induced oxidative stress and parthanatos in rat Schwann cells in vitro. Molecular Medicine Reports, 19, 338-344. https://doi.org/10.3892/mmr.2018.9631
MLA
Li, Q., Jiao, Y., Yu, Y., Wang, G., Yu, Y."Hydrogen‑rich medium alleviates high glucose‑induced oxidative stress and parthanatos in rat Schwann cells in vitro". Molecular Medicine Reports 19.1 (2019): 338-344.
Chicago
Li, Q., Jiao, Y., Yu, Y., Wang, G., Yu, Y."Hydrogen‑rich medium alleviates high glucose‑induced oxidative stress and parthanatos in rat Schwann cells in vitro". Molecular Medicine Reports 19, no. 1 (2019): 338-344. https://doi.org/10.3892/mmr.2018.9631
Copy and paste a formatted citation
x
Spandidos Publications style
Li Q, Jiao Y, Yu Y, Wang G and Yu Y: Hydrogen‑rich medium alleviates high glucose‑induced oxidative stress and parthanatos in rat Schwann cells in vitro. Mol Med Rep 19: 338-344, 2019.
APA
Li, Q., Jiao, Y., Yu, Y., Wang, G., & Yu, Y. (2019). Hydrogen‑rich medium alleviates high glucose‑induced oxidative stress and parthanatos in rat Schwann cells in vitro. Molecular Medicine Reports, 19, 338-344. https://doi.org/10.3892/mmr.2018.9631
MLA
Li, Q., Jiao, Y., Yu, Y., Wang, G., Yu, Y."Hydrogen‑rich medium alleviates high glucose‑induced oxidative stress and parthanatos in rat Schwann cells in vitro". Molecular Medicine Reports 19.1 (2019): 338-344.
Chicago
Li, Q., Jiao, Y., Yu, Y., Wang, G., Yu, Y."Hydrogen‑rich medium alleviates high glucose‑induced oxidative stress and parthanatos in rat Schwann cells in vitro". Molecular Medicine Reports 19, no. 1 (2019): 338-344. https://doi.org/10.3892/mmr.2018.9631
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