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Article Open Access

Genome‑wide DNA methylation analysis of uterosacral ligaments in women with pelvic organ prolapse

Corrigendum in: /10.3892/mmr.2019.9852
  • Authors:
    • Lifang Zhang
    • Ping Zheng
    • Aihong Duan
    • Yan Hao
    • Chang Lu
    • Dan Lu
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 391-399
    |
    Published online on: November 13, 2018
       https://doi.org/10.3892/mmr.2018.9656
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Abstract

Pelvic organ prolapse (POP) is an increasingly serious health problem that impairs quality of life and is caused by multiple additive genetic and environmental factors. As the uterosacral ligaments (ULs) provide primary support for the pelvic organs, it was hypothesized that disruption of these ligaments (as a result of aberrant methylation) may lead to a loss of support and eventually contribute to POP. In the present study, whether there are any aberrant methylations in the ULs of patients with POP compared to those of controls was investigated. Genomic DNA was isolated from the ULs of five women with POP and four women without POP, as controls, undergoing hysterectomy for benign conditions. An Illumina Infinium Methylation EPICBeadChips Infinium Human Methylation 850 K bead array was used to investigate the total methylation in the ULs. There were 3,723 differentially methylated CpG sites (Δβ<0.14; P<0.05), including 3,576 hypermethylation and 147 hypomethylation sites in the ULs of patients with POP compared with the normal controls. There were more hypermethylated CpG sites, but a high ratio of hypomethylation between CpG islands and the N‑shelf; in the gene structure, there was more hypermethylation than hypomethylation in TSS1500 and the 5' untranslated region. Gene ontology analysis demonstrated that these differentially methylated genes were associated with ‘cell morphogenesis’, ‘extracellular matrix’, ‘cell junction’, ‘protein binding’ and ‘guanosine triphosphatase activity’. Several significant pathways were identified, including ‘focal adhesion’ and ‘extracellular matrix‑receptor interaction pathway’. This study provides evidence that there are differences in genome‑wide DNA methylation between ULs in menopausal women with and without POP, and that epigenetic mechanisms may partly contribute to POP pathogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Zheng P, Duan A, Hao Y, Lu C and Lu D: Genome‑wide DNA methylation analysis of uterosacral ligaments in women with pelvic organ prolapse Corrigendum in /10.3892/mmr.2019.9852. Mol Med Rep 19: 391-399, 2019.
APA
Zhang, L., Zheng, P., Duan, A., Hao, Y., Lu, C., & Lu, D. (2019). Genome‑wide DNA methylation analysis of uterosacral ligaments in women with pelvic organ prolapse Corrigendum in /10.3892/mmr.2019.9852. Molecular Medicine Reports, 19, 391-399. https://doi.org/10.3892/mmr.2018.9656
MLA
Zhang, L., Zheng, P., Duan, A., Hao, Y., Lu, C., Lu, D."Genome‑wide DNA methylation analysis of uterosacral ligaments in women with pelvic organ prolapse Corrigendum in /10.3892/mmr.2019.9852". Molecular Medicine Reports 19.1 (2019): 391-399.
Chicago
Zhang, L., Zheng, P., Duan, A., Hao, Y., Lu, C., Lu, D."Genome‑wide DNA methylation analysis of uterosacral ligaments in women with pelvic organ prolapse Corrigendum in /10.3892/mmr.2019.9852". Molecular Medicine Reports 19, no. 1 (2019): 391-399. https://doi.org/10.3892/mmr.2018.9656
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Zheng P, Duan A, Hao Y, Lu C and Lu D: Genome‑wide DNA methylation analysis of uterosacral ligaments in women with pelvic organ prolapse Corrigendum in /10.3892/mmr.2019.9852. Mol Med Rep 19: 391-399, 2019.
APA
Zhang, L., Zheng, P., Duan, A., Hao, Y., Lu, C., & Lu, D. (2019). Genome‑wide DNA methylation analysis of uterosacral ligaments in women with pelvic organ prolapse Corrigendum in /10.3892/mmr.2019.9852. Molecular Medicine Reports, 19, 391-399. https://doi.org/10.3892/mmr.2018.9656
MLA
Zhang, L., Zheng, P., Duan, A., Hao, Y., Lu, C., Lu, D."Genome‑wide DNA methylation analysis of uterosacral ligaments in women with pelvic organ prolapse Corrigendum in /10.3892/mmr.2019.9852". Molecular Medicine Reports 19.1 (2019): 391-399.
Chicago
Zhang, L., Zheng, P., Duan, A., Hao, Y., Lu, C., Lu, D."Genome‑wide DNA methylation analysis of uterosacral ligaments in women with pelvic organ prolapse Corrigendum in /10.3892/mmr.2019.9852". Molecular Medicine Reports 19, no. 1 (2019): 391-399. https://doi.org/10.3892/mmr.2018.9656
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