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Article

Knockdown of P120 catenin aggravates endothelial injury under an impinging flow by inducing breakdown of adherens junctions

  • Authors:
    • Jian‑Lan Zhao
    • Zhi‑Peng Xiao
    • Nian‑Zu Yu
    • Jin‑Wen Jiang
    • Mei‑Hua Li
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China, Department of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University, Shanghai 200040, P.R. China
  • Pages: 541-548
    |
    Published online on: November 13, 2018
       https://doi.org/10.3892/mmr.2018.9657
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Abstract

At present, the mechanisms underlying intracranial aneurysm (IA) development remain unclear; however, hemodynamics is considered a crucial factor in the induction of IA. To elucidate the association between hemodynamics and endothelial cell (EC) functions, a modified T chamber system was designed to simulate the adjustable hemodynamic conditions of an artery bifurcation. Normal human umbilical vein ECs (HUVECs) and HUVECs with P120 catenin (P120ctn) knockdown were cultured on coverslips and placed in the chamber. A flow rate of 250 or 500 ml/min impinged on the cell layer. Subsequently, the expression levels of P120ctn and other proteins, and EC morphological alterations, were examined. In normal HUVECs, after 3 h under a flow rate of 500 ml/min, the expression levels of P120ctn, vascular endothelial (VE)‑Cadherin, Kaiso and α‑catenin were decreased, whereas matrix metalloproteinase‑2 (MMP‑2) was increased. In HUVECs with P120ctn knockdown, the period during which ECs adhered to the coverslip was reduced to 1 h under a flow rate of 500 ml/min. In addition, the expression levels of VE‑Cadherin, Kaiso and α‑catenin in ECs were decreased, whereas those of MMP‑2 were increased after 1 h; more prominent alterations were detected under a 500 ml/min flow rate compared with a 250 ml/min flow rate. Adherens junctions (AJs) are critical to the maintenance of normal morphology and EC functioning in the vascular wall, and P120ctn is an important regulator of AJs. Loss of P120ctn may be induced by hemodynamic alterations. In response to changes in hemodynamic conditions, a loss of P120ctn may aggravate AJs between ECs, thus inducing inflammation in the vascular wall. Clinically, hemodynamic alterations may result in a loss of P120ctn and endothelial injury; therefore, P120ctn may have a critical role in inducing intracranial aneurysms.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao JL, Xiao ZP, Yu NZ, Jiang JW and Li MH: Knockdown of P120 catenin aggravates endothelial injury under an impinging flow by inducing breakdown of adherens junctions. Mol Med Rep 19: 541-548, 2019.
APA
Zhao, J., Xiao, Z., Yu, N., Jiang, J., & Li, M. (2019). Knockdown of P120 catenin aggravates endothelial injury under an impinging flow by inducing breakdown of adherens junctions. Molecular Medicine Reports, 19, 541-548. https://doi.org/10.3892/mmr.2018.9657
MLA
Zhao, J., Xiao, Z., Yu, N., Jiang, J., Li, M."Knockdown of P120 catenin aggravates endothelial injury under an impinging flow by inducing breakdown of adherens junctions". Molecular Medicine Reports 19.1 (2019): 541-548.
Chicago
Zhao, J., Xiao, Z., Yu, N., Jiang, J., Li, M."Knockdown of P120 catenin aggravates endothelial injury under an impinging flow by inducing breakdown of adherens junctions". Molecular Medicine Reports 19, no. 1 (2019): 541-548. https://doi.org/10.3892/mmr.2018.9657
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao JL, Xiao ZP, Yu NZ, Jiang JW and Li MH: Knockdown of P120 catenin aggravates endothelial injury under an impinging flow by inducing breakdown of adherens junctions. Mol Med Rep 19: 541-548, 2019.
APA
Zhao, J., Xiao, Z., Yu, N., Jiang, J., & Li, M. (2019). Knockdown of P120 catenin aggravates endothelial injury under an impinging flow by inducing breakdown of adherens junctions. Molecular Medicine Reports, 19, 541-548. https://doi.org/10.3892/mmr.2018.9657
MLA
Zhao, J., Xiao, Z., Yu, N., Jiang, J., Li, M."Knockdown of P120 catenin aggravates endothelial injury under an impinging flow by inducing breakdown of adherens junctions". Molecular Medicine Reports 19.1 (2019): 541-548.
Chicago
Zhao, J., Xiao, Z., Yu, N., Jiang, J., Li, M."Knockdown of P120 catenin aggravates endothelial injury under an impinging flow by inducing breakdown of adherens junctions". Molecular Medicine Reports 19, no. 1 (2019): 541-548. https://doi.org/10.3892/mmr.2018.9657
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