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Article Open Access

Rosuvastatin protects against oxidized low‑density lipoprotein‑induced endothelial cell injury of atherosclerosis in vitro

  • Authors:
    • Jianan Geng
    • Huali Xu
    • Xiaofeng Yu
    • Guoliang Xu
    • Hongyan Cao
    • Guangzhu Lin
    • Dayun Sui
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, College of Pharmacy, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Cardiovascular Medicine, The Eastern Division of First Hospital, Jilin University, Changchun, Jilin 130031, P.R. China
    Copyright: © Geng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 432-440
    |
    Published online on: November 19, 2018
       https://doi.org/10.3892/mmr.2018.9666
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Abstract

Atherosclerosis‑induced cardiovascular diseases (CVDs) are accompanied by substantial morbidity and mortality. The loss and injury of endothelial cells is the primary cause of atherosclerosis. Rosuvastatin is an alternative agent used to reduce the risk of cardiovascular disease. Subsequently, the present study aimed to investigate the protective effects of rosuvastatin on oxidized‑low‑density lipoprotein (ox‑LDL)‑induced human umbilical vein endothelial cell (HUVEC) injury. The viability of ox‑LDL‑cultured HUVECs with or without rosuvastatin (0.01, 0.1 and 1 µmol/l) pretreatment, and pretreatment at different time points (3, 6, 12 and 24 h) was determined using an MTT assay. Morphological changes and the extent of apoptosis were detected; the anti‑oxidase activity, including superoxide dismutase (SOD) and catalase (CAT), was examined, and the contents of malondiahdehyde (MDA) and nitric oxide (NO) were measured. The phosphorylation levels of endothelial nitric oxide synthase (eNOS), protein kinase B (Akt) and phosphoinositide 3 kinase (PI3K) were detected using western blot analysis. The results demonstrated that pretreatment with 0.01‑1 µmol/l rosuvastatin decreased cell apoptosis caused by ox‑LDL. Notably, pretreatment with 1 µmol/l rosuvastatin for >12 h increased cell viability. Additionally, DAPI staining revealed that rosuvastatin inhibited HUVEC apoptosis. Rosuvastatin treatment also resulted in increased SOD and CAT activities and decreased MDA content in ox‑LDL‑stimulated HUVECs. Furthermore, pretreatment with 0.01‑1 µmol/l rosuvastatin significantly increased` the NO content compared with HUVECs treated with ox‑LDL alone. Western blot analyses demonstrated that rosuvastatin upregulated the phosphorylation of eNOS, Akt and PI3K. These findings indicated that rosuvastatin could protect HUVECs against ox‑LDL‑induced injury through its anti‑oxidant effect and its ability to upregulate the expression of vascular endotheliocyte‑protecting factors.
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Copy and paste a formatted citation
Spandidos Publications style
Geng J, Xu H, Yu X, Xu G, Cao H, Lin G and Sui D: Rosuvastatin protects against oxidized low‑density lipoprotein‑induced endothelial cell injury of atherosclerosis in vitro. Mol Med Rep 19: 432-440, 2019.
APA
Geng, J., Xu, H., Yu, X., Xu, G., Cao, H., Lin, G., & Sui, D. (2019). Rosuvastatin protects against oxidized low‑density lipoprotein‑induced endothelial cell injury of atherosclerosis in vitro. Molecular Medicine Reports, 19, 432-440. https://doi.org/10.3892/mmr.2018.9666
MLA
Geng, J., Xu, H., Yu, X., Xu, G., Cao, H., Lin, G., Sui, D."Rosuvastatin protects against oxidized low‑density lipoprotein‑induced endothelial cell injury of atherosclerosis in vitro". Molecular Medicine Reports 19.1 (2019): 432-440.
Chicago
Geng, J., Xu, H., Yu, X., Xu, G., Cao, H., Lin, G., Sui, D."Rosuvastatin protects against oxidized low‑density lipoprotein‑induced endothelial cell injury of atherosclerosis in vitro". Molecular Medicine Reports 19, no. 1 (2019): 432-440. https://doi.org/10.3892/mmr.2018.9666
Copy and paste a formatted citation
x
Spandidos Publications style
Geng J, Xu H, Yu X, Xu G, Cao H, Lin G and Sui D: Rosuvastatin protects against oxidized low‑density lipoprotein‑induced endothelial cell injury of atherosclerosis in vitro. Mol Med Rep 19: 432-440, 2019.
APA
Geng, J., Xu, H., Yu, X., Xu, G., Cao, H., Lin, G., & Sui, D. (2019). Rosuvastatin protects against oxidized low‑density lipoprotein‑induced endothelial cell injury of atherosclerosis in vitro. Molecular Medicine Reports, 19, 432-440. https://doi.org/10.3892/mmr.2018.9666
MLA
Geng, J., Xu, H., Yu, X., Xu, G., Cao, H., Lin, G., Sui, D."Rosuvastatin protects against oxidized low‑density lipoprotein‑induced endothelial cell injury of atherosclerosis in vitro". Molecular Medicine Reports 19.1 (2019): 432-440.
Chicago
Geng, J., Xu, H., Yu, X., Xu, G., Cao, H., Lin, G., Sui, D."Rosuvastatin protects against oxidized low‑density lipoprotein‑induced endothelial cell injury of atherosclerosis in vitro". Molecular Medicine Reports 19, no. 1 (2019): 432-440. https://doi.org/10.3892/mmr.2018.9666
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