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Potential functions of long non‑coding RNAs in the osteogenic differentiation of human bone marrow mesenchymal stem cells

  • Authors:
    • Xiang Sun
    • Bo Jia
    • Xiao‑Ling Qiu
    • Hong‑Xing Chu
    • Zhao‑Qiang Zhang
    • Zhi‑Ping Wang
    • Jian‑Jiang Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University (Guangdong Provincial Stomatological Hospital), Guangzhou, Guangdong 510280, P.R. China, Department of Endodontics, Stomatological Hospital, Southern Medical University (Guangdong Provincial Stomatological Hospital), Guangzhou, Guangdong 510280, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 103-114
    |
    Published online on: November 20, 2018
       https://doi.org/10.3892/mmr.2018.9674
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Abstract

Long non‑coding RNAs (lncRNAs) are a specific group of RNA molecules that do not encode proteins. They have been shown to serve important regulatory functions in various biological and cell differentiation processes. However, the potential functions and regulatory mechanisms of lncRNAs that are associated with the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) remain to be elucidated. The present study aimed to investigate lncRNAs that are differentially expressed during the osteogenic differentiation of hBMSCs, along with the potential functions of those lncRNAs. To this end, three groups of hBMSCs were stimulated to undergo osteogenic differentiation for 7 days. Known lncRNAs, unknown lncRNAs and mRNAs that demonstrated differential expression prior to and following the osteogenic differentiation of hBMSCs were screened using lncRNA high‑throughput sequencing. In addition, 12 lncRNAs were selected for reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) validation of the accuracy of the sequencing results. The potential functions and possible targets of the differentially expressed lncRNAs were analyzed using bioinformatics technologies (gene ontology, Kyoto Encyclopedia of Genes and Genomes and gene co‑expression network analysis). In total, 64 lncRNAs were differentially expressed by at least two‑fold in hBMSCs prior to and following osteogenic differentiation; these included seven known lncRNAs (two upregulated and five downregulated lncRNAs) and 57 unknown lncRNAs (35 upregulated and 22 downregulated lncRNAs). In addition, 409 mRNAs (257 upregulated and 152 downregulated mRNAs) were differentially expressed by at least two‑fold. The RT‑qPCR results obtained for 12 selected differentially expressed lncRNAs were consistent with the sequencing results. The gene co‑expression network analysis of lncRNAs and mRNAs demonstrated that four lncRNAs (ENSG00000238042, lnc_1269, lnc_1369 and lnc_1708) may serve important roles in the osteogenic differentiation of hBMSCs. In conclusion, during the osteogenic differentiation of hBMSCs, the lncRNA expression profile changed significantly; certain of the observed differentially expressed lncRNAs may be derived from protein‑coding genes and may serve important roles in osteogenic differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Sun X, Jia B, Qiu XL, Chu HX, Zhang ZQ, Wang ZP and Zhao JJ: Potential functions of long non‑coding RNAs in the osteogenic differentiation of human bone marrow mesenchymal stem cells. Mol Med Rep 19: 103-114, 2019.
APA
Sun, X., Jia, B., Qiu, X., Chu, H., Zhang, Z., Wang, Z., & Zhao, J. (2019). Potential functions of long non‑coding RNAs in the osteogenic differentiation of human bone marrow mesenchymal stem cells. Molecular Medicine Reports, 19, 103-114. https://doi.org/10.3892/mmr.2018.9674
MLA
Sun, X., Jia, B., Qiu, X., Chu, H., Zhang, Z., Wang, Z., Zhao, J."Potential functions of long non‑coding RNAs in the osteogenic differentiation of human bone marrow mesenchymal stem cells". Molecular Medicine Reports 19.1 (2019): 103-114.
Chicago
Sun, X., Jia, B., Qiu, X., Chu, H., Zhang, Z., Wang, Z., Zhao, J."Potential functions of long non‑coding RNAs in the osteogenic differentiation of human bone marrow mesenchymal stem cells". Molecular Medicine Reports 19, no. 1 (2019): 103-114. https://doi.org/10.3892/mmr.2018.9674
Copy and paste a formatted citation
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Spandidos Publications style
Sun X, Jia B, Qiu XL, Chu HX, Zhang ZQ, Wang ZP and Zhao JJ: Potential functions of long non‑coding RNAs in the osteogenic differentiation of human bone marrow mesenchymal stem cells. Mol Med Rep 19: 103-114, 2019.
APA
Sun, X., Jia, B., Qiu, X., Chu, H., Zhang, Z., Wang, Z., & Zhao, J. (2019). Potential functions of long non‑coding RNAs in the osteogenic differentiation of human bone marrow mesenchymal stem cells. Molecular Medicine Reports, 19, 103-114. https://doi.org/10.3892/mmr.2018.9674
MLA
Sun, X., Jia, B., Qiu, X., Chu, H., Zhang, Z., Wang, Z., Zhao, J."Potential functions of long non‑coding RNAs in the osteogenic differentiation of human bone marrow mesenchymal stem cells". Molecular Medicine Reports 19.1 (2019): 103-114.
Chicago
Sun, X., Jia, B., Qiu, X., Chu, H., Zhang, Z., Wang, Z., Zhao, J."Potential functions of long non‑coding RNAs in the osteogenic differentiation of human bone marrow mesenchymal stem cells". Molecular Medicine Reports 19, no. 1 (2019): 103-114. https://doi.org/10.3892/mmr.2018.9674
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