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Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity

  • Authors:
    • Meiying Feng
    • Nannan Dang
    • Yinshan Bai
    • Hengxi Wei
    • Li Meng
    • Kai Wang
    • Zhihong Zhao
    • Yun Chen
    • Fenglei Gao
    • Zhilin Chen
    • Li Li
    • Shouquan Zhang
  • View Affiliations / Copyright

    Affiliations: College of Animal Science, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro‑Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 155-164
    |
    Published online on: November 20, 2018
       https://doi.org/10.3892/mmr.2018.9675
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Abstract

Pronuclear migration, which is the initial stage of embryonic development and the marker of zygote formation, is a crucial process during mammalian preimplantation embryonic development. Recent studies have revealed that long non‑coding RNAs (lncRNAs) serve an important role in early embryonic development. However, the functional regulation of lncRNAs in this process has yet to be elucidated, largely due to the difficulty of assessing gene expression alterations during the very short time in which pronuclear migration occurs. It has previously been reported that migration of the pronucleus of a zygote can be obstructed by simulated microgravity. To investigate pronuclear migration in mice, a rotary cell culture system was employed, which generates simulated microgravity, in order to interfere with murine pronuclear migration. Subsequently, lncRNA sequencing was performed to investigate the mechanism underlying this process. In the present study, a comprehensive analysis of lncRNA profile during the mouse pronuclear stage was conducted, in which 3,307 lncRNAs were identified based on single‑cell RNA sequencing data. Furthermore, 52 lncRNAs were identified that were significantly differentially expressed. Subsequently, 10 lncRNAs were selected for validation by reverse transcription‑quantitative polymerase chain reaction, in which the same relative expression pattern was observed. The results revealed that 12 lncRNAs (lnc006745, lnc007956, lnc013100, lnc013782, lnc017097, lnc019869, lnc025838, lnc027046, lnc005454, lnc007956, lnc019410 and lnc019607), with tubulin β 4B class IVb or actinin α 4 as target genes, may be associated with the expression of microtubule and microfilament proteins. Binding association was confirmed using a dual‑luciferase reporter assay. Finally, Gene Ontology analysis revealed that the target genes of the differentially expressed lncRNAs participated in cellular processes associated with protein transport, binding, catalytic activity, membrane‑bounded organelle, protein complex and the cortical cytoskeleton. These findings suggested that these lncRNAs may be associated with migration of the mouse pronucleus.
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Copy and paste a formatted citation
Spandidos Publications style
Feng M, Dang N, Bai Y, Wei H, Meng L, Wang K, Zhao Z, Chen Y, Gao F, Chen Z, Chen Z, et al: Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity. Mol Med Rep 19: 155-164, 2019.
APA
Feng, M., Dang, N., Bai, Y., Wei, H., Meng, L., Wang, K. ... Zhang, S. (2019). Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity. Molecular Medicine Reports, 19, 155-164. https://doi.org/10.3892/mmr.2018.9675
MLA
Feng, M., Dang, N., Bai, Y., Wei, H., Meng, L., Wang, K., Zhao, Z., Chen, Y., Gao, F., Chen, Z., Li, L., Zhang, S."Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity". Molecular Medicine Reports 19.1 (2019): 155-164.
Chicago
Feng, M., Dang, N., Bai, Y., Wei, H., Meng, L., Wang, K., Zhao, Z., Chen, Y., Gao, F., Chen, Z., Li, L., Zhang, S."Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity". Molecular Medicine Reports 19, no. 1 (2019): 155-164. https://doi.org/10.3892/mmr.2018.9675
Copy and paste a formatted citation
x
Spandidos Publications style
Feng M, Dang N, Bai Y, Wei H, Meng L, Wang K, Zhao Z, Chen Y, Gao F, Chen Z, Chen Z, et al: Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity. Mol Med Rep 19: 155-164, 2019.
APA
Feng, M., Dang, N., Bai, Y., Wei, H., Meng, L., Wang, K. ... Zhang, S. (2019). Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity. Molecular Medicine Reports, 19, 155-164. https://doi.org/10.3892/mmr.2018.9675
MLA
Feng, M., Dang, N., Bai, Y., Wei, H., Meng, L., Wang, K., Zhao, Z., Chen, Y., Gao, F., Chen, Z., Li, L., Zhang, S."Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity". Molecular Medicine Reports 19.1 (2019): 155-164.
Chicago
Feng, M., Dang, N., Bai, Y., Wei, H., Meng, L., Wang, K., Zhao, Z., Chen, Y., Gao, F., Chen, Z., Li, L., Zhang, S."Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity". Molecular Medicine Reports 19, no. 1 (2019): 155-164. https://doi.org/10.3892/mmr.2018.9675
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