Rapamycin‑induced autophagy attenuates hormone‑imbalance‑induced chronic non‑bacterial prostatitis in rats via the inhibition of NLRP3 inflammasome‑mediated inflammation

Lu,Jingxiao; Su,Yang; Chen,Xianguo; Chen,Yuan; Luo,Pengcheng; Lin,Fangyou; Zhang,Jie

doi:10.3892/mmr.2018.9683

January-2019 Volume 19 Issue 1

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January-2019 Volume 19 Issue 1

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Rapamycin‑induced autophagy attenuates hormone‑imbalance‑induced chronic non‑bacterial prostatitis in rats via the inhibition of NLRP3 inflammasome‑mediated inflammation

Authors:
- Jingxiao Lu
- Yang Su
- Xianguo Chen
- Yuan Chen
- Pengcheng Luo
- Fangyou Lin
- Jie Zhang
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Affiliations: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China, Department of Clinical Laboratory, Children and Women Hospital of Edong Health Group, Huangshi, Hubei 435000, P.R. China, Department of Urology, Huangshi Central Hospital, Hubei Polytechnic University, Huangshi, Hubei 435000, P.R. China

Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 221-230
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Published online on: November 21, 2018

https://doi.org/10.3892/mmr.2018.9683
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Abstract

Chronic non‑bacterial prostatitis (CNBP) is a common urinary disease and no standard treatments are available at present. Although autophagy serves an important role in a variety of chronic diseases, its role in CNBP is yet to be fully elucidated. Therefore, the present study aimed to investigate the effects of rapamycin‑induced autophagy on CNBP by establishing a rat model. In the present study, a total of 30 male Sprague‑Dawley rats were randomly divided into three groups (n=10 per group): i) Control, in which rats underwent a sham operation; ii) the model (CNBP), in which rats were castrated and administered 17β‑estradiol (0.25 mg/kg via subcutaneous injection) for 30 consecutive days; and iii) rapamycin treatment, in which rats were employed in accordance with the CNBP model, but also received a daily intraperitoneal injection of rapamycin (1 mg/kg) from the 16th day post‑surgery for 15 days. Alterations in histology and the levels of autophagy‑associated markers, and components of the NLRP3 inflammasome, were measured in the prostate tissues of the rats. The levels of molecules located further downstream of the NLRP3 inflammasome pathway, including interleukin (IL)‑1β and IL‑18, were also measured. The results demonstrated that, compared with the control group, increased infiltration levels of inflammatory cells and glandular epithelial degeneration were observed in the prostate tissues of rats with CNBP. Furthermore, a significant increase in the concentration of IL‑1β and IL‑18 in the serum, as well as the increased expression levels of NLRP3, ASC and caspase‑1 in prostate tissues were also observed. In addition, reductions in the number of autophagosomes and the expression levels of autophagy‑associated, including microtubule‑associated protein 1 light chain 3β (LC3B) and Beclin 1, were also detected in the CNBP group; however, treatment with rapamycin reversed these effects. Collectively, the findings of the present study indicated that the NLRP3 inflammasome‑mediated inflammatory response was activated by a hormonal imbalance in the prostate glands of rats; however, these effects may be suppressed via rapamycin‑induced autophagy.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

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Medicine International

Rapamycin‑induced autophagy attenuates hormone‑imbalance‑induced chronic non‑bacterial prostatitis in rats via the inhibition of NLRP3 inflammasome‑mediated inflammation

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