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Article Open Access

Platelet‑derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells

  • Authors:
    • Jianbo Zhang
    • Haolong Zhang
    • Yikuan Chen
    • Jian Fu
    • Yu Lei
    • Jianming Sun
    • Bo Tang
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 125-132
    |
    Published online on: November 26, 2018
       https://doi.org/10.3892/mmr.2018.9692
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Abstract

Neovascularization and re-endothelialization rely on endothelial progenitor cells (EPCs). However, the recruitment and angiogenic roles of EPCs are subject to regulation through the vascular microenvironment, which remains largely unknown. Platelet‑derived growth factor D (PDGF‑D) is a new member of the PDGF family that binds the PDGFR‑β homodimer. However, it remains unknown whether and how it affects the angiogenic capacity of EPCs and participates in tube‑like formation. EPCs were derived from rat bone marrow cells, and the gain‑of‑function approach was used to study the effects of PDGF‑D on the biological activities of EPCs. EPCs that stably express PDGF‑D were generated by lentiviral‑mediated transduction, and the expression levels were evaluated by western blotting and reverse transcription, followed by real‑time quantitative polymerase chain reaction (RT‑qPCR). The biological activities of EPCs evaluated in the present study included proliferation, adhesion, migration, tube formation and senescence. Furthermore, the downstream signaling of PDGF‑D was explored by western blot analysis. The results revealed that the lentiviral‑mediated expression of PDGF‑D in the microenvironment promoted the migration, proliferation, adhesion and tube formation of EPCs. In addition, PDGF‑D suppressed the senescence of EPCs. Mechanistically, PDGF‑D induced the phosphorylation of several signaling molecules, including STAT3, AKT, ERK1/2, mTOR and GSK‑3β, suggesting that PDGF‑D enhanced the angiogenic function of EPCs through PDGF receptor‑dependent and ‑independent signaling pathways. In conclusion, PDGF‑D promotes the angiogenic capacity of EPCs, including proliferation, migration, adhesion and tube formation, and thereby contributes to angiogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Zhang H, Chen Y, Fu J, Lei Y, Sun J and Tang B: Platelet‑derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells. Mol Med Rep 19: 125-132, 2019.
APA
Zhang, J., Zhang, H., Chen, Y., Fu, J., Lei, Y., Sun, J., & Tang, B. (2019). Platelet‑derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells. Molecular Medicine Reports, 19, 125-132. https://doi.org/10.3892/mmr.2018.9692
MLA
Zhang, J., Zhang, H., Chen, Y., Fu, J., Lei, Y., Sun, J., Tang, B."Platelet‑derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells". Molecular Medicine Reports 19.1 (2019): 125-132.
Chicago
Zhang, J., Zhang, H., Chen, Y., Fu, J., Lei, Y., Sun, J., Tang, B."Platelet‑derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells". Molecular Medicine Reports 19, no. 1 (2019): 125-132. https://doi.org/10.3892/mmr.2018.9692
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Zhang H, Chen Y, Fu J, Lei Y, Sun J and Tang B: Platelet‑derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells. Mol Med Rep 19: 125-132, 2019.
APA
Zhang, J., Zhang, H., Chen, Y., Fu, J., Lei, Y., Sun, J., & Tang, B. (2019). Platelet‑derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells. Molecular Medicine Reports, 19, 125-132. https://doi.org/10.3892/mmr.2018.9692
MLA
Zhang, J., Zhang, H., Chen, Y., Fu, J., Lei, Y., Sun, J., Tang, B."Platelet‑derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells". Molecular Medicine Reports 19.1 (2019): 125-132.
Chicago
Zhang, J., Zhang, H., Chen, Y., Fu, J., Lei, Y., Sun, J., Tang, B."Platelet‑derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells". Molecular Medicine Reports 19, no. 1 (2019): 125-132. https://doi.org/10.3892/mmr.2018.9692
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