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Article

Characterization of circRNA‑associated ceRNA networks in patients with nonvalvular persistent atrial fibrillation

  • Authors:
    • Yangyang Zhang
    • Xianting Ke
    • Jingjing Liu
    • Xiue Ma
    • Yi Liu
    • Dandan Liang
    • Luxin Wang
    • Changfa Guo
    • Yiwei Luo
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Arrhythmias of The Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China, Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China
  • Pages: 638-650
    |
    Published online on: November 26, 2018
       https://doi.org/10.3892/mmr.2018.9695
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Abstract

Circular RNAs (circRNAs) are non-coding RNAs forming closed-loop structures, and their aberrant expression may lead to disease. However, the potential network of circRNA‑associated competing endogenous RNA (ceRNA) involved in nonvalvular persistent atrial fibrillation (NPAF) has not been previously reported. In the present study, four left atrial appendages (LAA) of patients with NPAF and four normal LAAs were examined via RNA sequencing, and their potential functions were investigated via bioinformatics analysis. The circRNA‑enriched genes were analyzed using Gene Ontology (GO) categories, while the enrichment of circRNAs was detected via the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A total of 296 significantly dysregulated circRNA transcripts were obtained, with 238 upregulated and 58 downregulated. A number of circRNAs were further confirmed using reverse transcription‑quantitative polymerase chain reaction analysis. Furthermore, the more comprehensive circRNA‑associated ceRNA networks were examined in patients with NPAF. GO categories and KEGG annotation analysis of circRNAs revealed that the circRNA‑associated ceRNA networks were likely to influence AF though alterations in calcium and cardiac muscle contraction. The circRNA‑associated ceRNA networks revealed that dysregulated circRNAs in NPAF may be involved in regulating hsa‑microRNA (miR)‑208b and hsa‑miR‑21. To the best of our knowledge, this study presents the circRNA‑associated ceRNA networks in NPAF for the first time, which may have potential implications for the pathogenesis of AF. This study reveals a potential perspective from which to investigate circRNAs in circRNA‑associated ceRNA networks (hsa_circRNA002085, hsa_circRNA001321) in NPAF, and provides a potential biomarker for AF.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Ke X, Liu J, Ma X, Liu Y, Liang D, Wang L, Guo C and Luo Y: Characterization of circRNA‑associated ceRNA networks in patients with nonvalvular persistent atrial fibrillation. Mol Med Rep 19: 638-650, 2019.
APA
Zhang, Y., Ke, X., Liu, J., Ma, X., Liu, Y., Liang, D. ... Luo, Y. (2019). Characterization of circRNA‑associated ceRNA networks in patients with nonvalvular persistent atrial fibrillation. Molecular Medicine Reports, 19, 638-650. https://doi.org/10.3892/mmr.2018.9695
MLA
Zhang, Y., Ke, X., Liu, J., Ma, X., Liu, Y., Liang, D., Wang, L., Guo, C., Luo, Y."Characterization of circRNA‑associated ceRNA networks in patients with nonvalvular persistent atrial fibrillation". Molecular Medicine Reports 19.1 (2019): 638-650.
Chicago
Zhang, Y., Ke, X., Liu, J., Ma, X., Liu, Y., Liang, D., Wang, L., Guo, C., Luo, Y."Characterization of circRNA‑associated ceRNA networks in patients with nonvalvular persistent atrial fibrillation". Molecular Medicine Reports 19, no. 1 (2019): 638-650. https://doi.org/10.3892/mmr.2018.9695
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Ke X, Liu J, Ma X, Liu Y, Liang D, Wang L, Guo C and Luo Y: Characterization of circRNA‑associated ceRNA networks in patients with nonvalvular persistent atrial fibrillation. Mol Med Rep 19: 638-650, 2019.
APA
Zhang, Y., Ke, X., Liu, J., Ma, X., Liu, Y., Liang, D. ... Luo, Y. (2019). Characterization of circRNA‑associated ceRNA networks in patients with nonvalvular persistent atrial fibrillation. Molecular Medicine Reports, 19, 638-650. https://doi.org/10.3892/mmr.2018.9695
MLA
Zhang, Y., Ke, X., Liu, J., Ma, X., Liu, Y., Liang, D., Wang, L., Guo, C., Luo, Y."Characterization of circRNA‑associated ceRNA networks in patients with nonvalvular persistent atrial fibrillation". Molecular Medicine Reports 19.1 (2019): 638-650.
Chicago
Zhang, Y., Ke, X., Liu, J., Ma, X., Liu, Y., Liang, D., Wang, L., Guo, C., Luo, Y."Characterization of circRNA‑associated ceRNA networks in patients with nonvalvular persistent atrial fibrillation". Molecular Medicine Reports 19, no. 1 (2019): 638-650. https://doi.org/10.3892/mmr.2018.9695
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