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Article Open Access

Sphingomyelin synthase 1 regulates the epithelial‑to‑mesenchymal transition mediated by the TGF‑β/Smad pathway in MDA‑MB‑231 cells

  • Authors:
    • Shuang Liu
    • Huan Hou
    • Panpan Zhang
    • Yifan Wu
    • Xuanhong He
    • Hua Li
    • Nianlong Yan
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Biochemistry and Molecular Biology, Centre of Experimental Medicine, Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1159-1167
    |
    Published online on: December 4, 2018
       https://doi.org/10.3892/mmr.2018.9722
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Abstract

Breast cancer is the most common cancer in women and a leading cause of cancer‑associated mortalities in the world. Epithelial‑to‑mesenchymal transition (EMT) serves an important role in the process of metastasis and invasive ability in cancer cells, and transforming growth factor β1 (TGF‑β1) have been investigated for promoting EMT. However, in the present study, the role of the sphingomyelin synthase 1 (SMS1) in TGF‑β1‑induced EMT development was investigated. Firstly, bioinformatics analysis demonstrated that the overexpression of SMS1 negatively regulated the TGFβ receptor I (TβRI) level of expression. Subsequently, the expression of SMS1 was decreased, whereas, SMS2 had no significant difference when MDA‑MB‑231 cells were treated by TGF‑β1 for 72 h. Furthermore, the present study constructed an overexpression cells model of SMS1 and these cells were treated by TGF‑β1. These results demonstrated that overexpression of SMS1 inhibited TGF‑β1‑induced EMT and the migration and invasion of MDA‑MB‑231 cells, increasing the expression of E‑cadherin while decreasing the expression of vimentin. Furthermore, the present study further confirmed that SMS1 overexpression could decrease TβRI expression levels and blocked smad family member 2 phosphorylation. Overall, the present results suggested that SMS1 could inhibit EMT and the migration and invasion of MDA‑MB‑231 cells via TGF‑β/Smad signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Liu S, Hou H, Zhang P, Wu Y, He X, Li H and Yan N: Sphingomyelin synthase 1 regulates the epithelial‑to‑mesenchymal transition mediated by the TGF‑β/Smad pathway in MDA‑MB‑231 cells. Mol Med Rep 19: 1159-1167, 2019.
APA
Liu, S., Hou, H., Zhang, P., Wu, Y., He, X., Li, H., & Yan, N. (2019). Sphingomyelin synthase 1 regulates the epithelial‑to‑mesenchymal transition mediated by the TGF‑β/Smad pathway in MDA‑MB‑231 cells. Molecular Medicine Reports, 19, 1159-1167. https://doi.org/10.3892/mmr.2018.9722
MLA
Liu, S., Hou, H., Zhang, P., Wu, Y., He, X., Li, H., Yan, N."Sphingomyelin synthase 1 regulates the epithelial‑to‑mesenchymal transition mediated by the TGF‑β/Smad pathway in MDA‑MB‑231 cells". Molecular Medicine Reports 19.2 (2019): 1159-1167.
Chicago
Liu, S., Hou, H., Zhang, P., Wu, Y., He, X., Li, H., Yan, N."Sphingomyelin synthase 1 regulates the epithelial‑to‑mesenchymal transition mediated by the TGF‑β/Smad pathway in MDA‑MB‑231 cells". Molecular Medicine Reports 19, no. 2 (2019): 1159-1167. https://doi.org/10.3892/mmr.2018.9722
Copy and paste a formatted citation
x
Spandidos Publications style
Liu S, Hou H, Zhang P, Wu Y, He X, Li H and Yan N: Sphingomyelin synthase 1 regulates the epithelial‑to‑mesenchymal transition mediated by the TGF‑β/Smad pathway in MDA‑MB‑231 cells. Mol Med Rep 19: 1159-1167, 2019.
APA
Liu, S., Hou, H., Zhang, P., Wu, Y., He, X., Li, H., & Yan, N. (2019). Sphingomyelin synthase 1 regulates the epithelial‑to‑mesenchymal transition mediated by the TGF‑β/Smad pathway in MDA‑MB‑231 cells. Molecular Medicine Reports, 19, 1159-1167. https://doi.org/10.3892/mmr.2018.9722
MLA
Liu, S., Hou, H., Zhang, P., Wu, Y., He, X., Li, H., Yan, N."Sphingomyelin synthase 1 regulates the epithelial‑to‑mesenchymal transition mediated by the TGF‑β/Smad pathway in MDA‑MB‑231 cells". Molecular Medicine Reports 19.2 (2019): 1159-1167.
Chicago
Liu, S., Hou, H., Zhang, P., Wu, Y., He, X., Li, H., Yan, N."Sphingomyelin synthase 1 regulates the epithelial‑to‑mesenchymal transition mediated by the TGF‑β/Smad pathway in MDA‑MB‑231 cells". Molecular Medicine Reports 19, no. 2 (2019): 1159-1167. https://doi.org/10.3892/mmr.2018.9722
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