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Article

Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice

  • Authors:
    • Bo Chen
    • Baoshan Liu
    • Zhina Zhao
    • Guizhen Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pathogenic Biology, College of Basic Medical Science, China Medical University, Shenyang, Liaoning 110122, P.R. China, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning 110866, P.R. China, Department of Microbiology and Cell Biology, College of Life Science and Pharmaceuticals, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China
  • Pages: 1302-1308
    |
    Published online on: December 11, 2018
       https://doi.org/10.3892/mmr.2018.9735
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Abstract

Brucellosis is an important neglected zoonotic disease, and the pathogens responsible are Brucellae. In order to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella BvrR, the recombinant plasmid pCDNA‑BvrR was constructed by inserting the BvrR gene fragment into a pCDNA3.0 vector. The His6‑tagged BvrR was purified with His‑trap FF crude affinity chromatography and verified with an anti‑histidine monoclonal antibody by western blot analysis. The specific immunoglobulin antigens and their isotypes were detected by indirect ELISA. The recombinant His6‑BvrR protein was expressed and purified by affinity chromatography. The optical density 450 value of immunoglobulin G (IgG) in the pCDNA‑BvrR group was significantly increased compared with the pCDNA3.0 vector or PBS groups (P<0.05), and the pCDNA3.0 vector and PBS groups exhibited no significant difference (P>0.05). BvrR induced specific antibodies with a dominance of IgG2a over IgG1 and the T cell‑proliferative response, in addition to a typical T helper‑1 (Th1)‑dominated immune response in mice. The splenocytes from mice of the pCDNA‑BvrR group demonstrated significant proliferative activity compared with the pCDNA3.0 vector group. The present results indicated that immunization with BvrR induced a specific Th1‑type immune response in mice. Subsequent to challenging with B. abortus S19, it was identified that the DNA vaccine pCDNA‑BvrR induced a significant level of protection in BALB/c mice by evaluating systemic bacterial clearance. These results suggested that BvrR may be a good candidate for a DNA vaccine against brucellosis.
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Copy and paste a formatted citation
Spandidos Publications style
Chen B, Liu B, Zhao Z and Wang G: Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice. Mol Med Rep 19: 1302-1308, 2019.
APA
Chen, B., Liu, B., Zhao, Z., & Wang, G. (2019). Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice. Molecular Medicine Reports, 19, 1302-1308. https://doi.org/10.3892/mmr.2018.9735
MLA
Chen, B., Liu, B., Zhao, Z., Wang, G."Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice". Molecular Medicine Reports 19.2 (2019): 1302-1308.
Chicago
Chen, B., Liu, B., Zhao, Z., Wang, G."Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice". Molecular Medicine Reports 19, no. 2 (2019): 1302-1308. https://doi.org/10.3892/mmr.2018.9735
Copy and paste a formatted citation
x
Spandidos Publications style
Chen B, Liu B, Zhao Z and Wang G: Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice. Mol Med Rep 19: 1302-1308, 2019.
APA
Chen, B., Liu, B., Zhao, Z., & Wang, G. (2019). Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice. Molecular Medicine Reports, 19, 1302-1308. https://doi.org/10.3892/mmr.2018.9735
MLA
Chen, B., Liu, B., Zhao, Z., Wang, G."Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice". Molecular Medicine Reports 19.2 (2019): 1302-1308.
Chicago
Chen, B., Liu, B., Zhao, Z., Wang, G."Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice". Molecular Medicine Reports 19, no. 2 (2019): 1302-1308. https://doi.org/10.3892/mmr.2018.9735
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