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Article Open Access

Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs

  • Authors:
    • Xiping Yang
    • Yisheng Chen
    • Jianxin Li
    • Lijun Chen
    • Hefei Ren
    • Yang Liu
    • Xinyu Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Affiliated Hospital of Logistic University of Chinese People's Armed Police Force, Tianjin 300162, P.R. China, Department of Biochemistry and Physiology, Logistic University of Chinese People's Armed Police Force, Tianjin 300309, P.R. China, Graduate Management Team, Logistic University of Chinese People's Armed Police Force, Tianjin 300309, P.R. China, Department of Neurology, Shanghai Fourth People's Hospital, Shanghai 200000, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1083-1091
    |
    Published online on: December 12, 2018
       https://doi.org/10.3892/mmr.2018.9748
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Abstract

Traumatic brain injury (TBI) is the most common cause of death and permanent disability in people aged <45, and is associated with secondary brain injury and bleed progression, resulting in increased morbidity and mortality. TBI may also induce innate host defense responses characterized by activation of resident microglia and astrocytes, brain microvascular endothelial cells and peripheral blood monocytes. In the present study, 34 patients with moderate‑to‑severe traumatic brain injury were randomly divided into two groups, including a 7.5% hypertonic saline (HS) treatment group (4 ml/kg) and 3% HS treatment group (4 ml/kg). The results demonstrated that treatment with 7.5% HS decreased the intracranial pressure and improved coagulofibrinolytic homeostasis. Analysis of the monocyte subsets revealed significant reduction in the proportion of cluster of differentiation (CD)14++CD16+ circulating inflammatory monocytes in the 7.5% HS group. In addition, 7.5% HS treatment downregulated the expression of long non‑coding (lnc) RNA2448‑11 and lncRNA1403 in the peripheral blood mononuclear cells of patients with TBI. Using reverse transcription‑quantitative polymerase chain reaction, it was determined that 7.5% HS regulated the expression of tumor necrosis factor‑α, interleukin‑1β, transforming growth factor‑β and thrombomodulin, which are the target genes of lncRNA2448‑11 and lncRNA1403. These results indicated that 7.5% HS improved the intracranial pressure and coagulofibrinolytic homeostasis by modulating the phenotype of monocytes through lncRNA2448‑11 and lncRNA1403. These findings provided evidence that initial resuscitation with HS imparts functional changes to inflammatory cells following TBI, thereby reducing potential neuroinflammatory events associated with secondary brain injury.
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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Chen Y, Li J, Chen L, Ren H, Liu Y and Zhang X: Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs. Mol Med Rep 19: 1083-1091, 2019.
APA
Yang, X., Chen, Y., Li, J., Chen, L., Ren, H., Liu, Y., & Zhang, X. (2019). Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs. Molecular Medicine Reports, 19, 1083-1091. https://doi.org/10.3892/mmr.2018.9748
MLA
Yang, X., Chen, Y., Li, J., Chen, L., Ren, H., Liu, Y., Zhang, X."Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs". Molecular Medicine Reports 19.2 (2019): 1083-1091.
Chicago
Yang, X., Chen, Y., Li, J., Chen, L., Ren, H., Liu, Y., Zhang, X."Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs". Molecular Medicine Reports 19, no. 2 (2019): 1083-1091. https://doi.org/10.3892/mmr.2018.9748
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Chen Y, Li J, Chen L, Ren H, Liu Y and Zhang X: Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs. Mol Med Rep 19: 1083-1091, 2019.
APA
Yang, X., Chen, Y., Li, J., Chen, L., Ren, H., Liu, Y., & Zhang, X. (2019). Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs. Molecular Medicine Reports, 19, 1083-1091. https://doi.org/10.3892/mmr.2018.9748
MLA
Yang, X., Chen, Y., Li, J., Chen, L., Ren, H., Liu, Y., Zhang, X."Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs". Molecular Medicine Reports 19.2 (2019): 1083-1091.
Chicago
Yang, X., Chen, Y., Li, J., Chen, L., Ren, H., Liu, Y., Zhang, X."Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs". Molecular Medicine Reports 19, no. 2 (2019): 1083-1091. https://doi.org/10.3892/mmr.2018.9748
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