Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry

Ding,Jing‑Xin; Li,Wen‑Lan; Hu,Yang; Song,Hui; Sun,Xiang‑Ming; Ji,Yu‑Bin

doi:10.3892/mmr.2018.9755

Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry

Authors:
- Jing‑Xin Ding
- Wen‑Lan Li
- Yang Hu
- Hui Song
- Xiang‑Ming Sun
- Yu‑Bin Ji
View Affiliations

Affiliations: Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin, Heilongjiang 150076, P.R. China, College of Pharmacy, Harbin University of Commerce, Harbin, Heilongjiang 150076, P.R. China
Published online on: December 12, 2018 https://doi.org/10.3892/mmr.2018.9755
Pages: 1238-1247

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

High-performance liquid chromatography (HPLC) is an efficient method that is widely used to assess the quality of traditional Chinese medicine (TCM). It is well known that the quality of TCM has a direct effect on its efficacy; therefore, in order to thoroughly explain how TCM exerts its efficacy, it is necessary to characterize its active ingredients and assess their quality. The application of the spectrum‑effect method is crucial for determining the pharmacological basis of materials. The aim of the present study was to examine the correlation between chemical spectra and estrogenic activity of Cuscuta chinensis Lam., in order to reveal active compounds with potential therapeutic effects. The spectra of Cuscuta chinensis Lam. were recorded using HPLC, and estrogenic activity was determined using a uterus growth test and MTT assay. Combination of the results of bivariate analysis, principal component analysis and Gray relational analysis identified 19 active compounds, as follows: Quercetin‑3‑O‑(2‘‑O‑α‑rhamnosy‑6’‑O‑malony)‑β‑D‑glucoside, ka-empferol‑3‑O‑β‑D‑aplosyl‑(1→2)‑[‑α‑L‑rhamnosy‑(1→6)]‑β-wD-glucoside, 6‑O‑(E)‑P‑coumaroyl)‑β‑D‑fructofuranosyl‑(2→1)‑α‑D‑glucopyranoside, kaempferol‑7‑rhamnosy, kaempferol‑3‑β‑D-glucuronide, apigenin, 4‑caffeoyl‑5‑coumaroylquinic acid, kaempferol‑3‑arabofuranoside, quercetin‑3‑O-β‑D-apiofuranosyl-(1→2)-β‑D‑galactoside, dicaffeoylquinic acid, hyperin, quercitin, isorhamnetin, chlorogenic acid, quercetin, quercltrin‑2''‑gallate, quercetin‑3, 7‑α‑L‑dirhamnoside and stigmasterol, as well as one unknown compound. The present study laid a foundation for in vivo metabolic studies regarding Cuscuta chinensis Lam. and for the development of its clinical application.

View Figures

View References

1	An BH, Jeong H, Zhou W, Liu X, Kim S, Jang CY, Kim HS, Sohn J, Park HJ, Sung NH, et al: Evaluation of the biological activity of opuntia ficus indica as a tissue- and estrogen receptor subtype-selective modulator. Phytother Res. 30:971–980. 2016. View Article : Google Scholar : PubMed/NCBI
2	van de Schans MG, Vincken JP, de Waard P, Hamers AR, Bovee TF and Gruppen H: Glyceollins and dehydroglyceollins isolated from soybean act as SERMs and ER subtype-selective phytoestrogens. J Steroid Biochem Mol Biol. 156:53–63. 2016. View Article : Google Scholar : PubMed/NCBI
3	Wang Z, Xia Q, Liu X, Liu W, Huang W, Mei X, Luo J, Shan M, Lin R, Zou D and Ma Z: Phytochemistry, pharmacology, quality control and future research of Forsythia suspensa (Thunb.) Vahl: A review. J Ethnopharmacol. 210:318–339. 2018. View Article : Google Scholar : PubMed/NCBI
4	Shu Y, Liu Z, Zhao S, Song Z, He D, Wang M, Zeng H, Lu C, Lu A and Liu Y: Integrated and global pseudotargeted metabolomics strategy applied to screening for quality control markers of Citrus TCMs. Anal Bioanal Chem. 409:4849–4865. 2017. View Article : Google Scholar : PubMed/NCBI
5	Yang B, Wang Y, Shan L, Zou J, Wu Y, Yang F and Zhang Y, Li Y and Zhang Y: A novel and practical chromatographic ‘Fingerprint-ROC-SVM’ strategy applied to quality analysis of traditional chinese medicine injections: Using KuDieZi injection as a case study. Molecules. 22:E12372017. View Article : Google Scholar : PubMed/NCBI
6	Zhuo L, Peng J, Zhao Y, Li D, Xie X, Tong L and Yu Z: Screening bioactive quality control markers of QiShenYiQi dripping pills based on the relationship between the ultra-high performance liquid chromatography fingerprint and vascular protective activity. J Sep Sci. 40:4076–4084. 2017. View Article : Google Scholar : PubMed/NCBI
7	Spagnuolo P, Rasini E, Luini A, Legnaro M, Luzzani M, Casareto E, Carreri M, Paracchini S, Marino F and Cosentino M: Isoflavone content and estrogenic activity of different batches of red clover (Trifolium pratense L.) extracts: An in vitro study in MCF-7 cells. Fitoterapia. 94:62–69. 2014. View Article : Google Scholar : PubMed/NCBI
8	Wang DD, Liang J, Yang WZ, Hou JJ, Yang M, Da J, Wang Y, Jiang BH, Liu X, Wu WY and Guo DA: HPLC/qTOF-MS-oriented characteristic components data set and chemometric analysis for the holistic quality control of complex TCM preparations: Niuhuang Shangqing pill as an example. J Pharm Biomed Anal. 89:130–141. 2014. View Article : Google Scholar : PubMed/NCBI
9	Hernaez R and Thrift AP: High negative predictive value, low prevalence, and spectrum effect: Caution in the interpretation. Clin Gastroenterol Hepatol. 15:1355–1358. 2017. View Article : Google Scholar : PubMed/NCBI
10	Li W, Sun X, Liu B, Zhang L, Fan Z and Ji Y: Screening and identification of hepatotoxic component in Evodia rutaecarpa based on spectrum-effect relationship and UPLC-Q-TOFMS. Biomed Chromatogr. 30:1975–1983. 2016. View Article : Google Scholar : PubMed/NCBI
11	Zheng Q, Zhao Y, Wang J, Liu T, Zhang B, Gong M, Li J, Liu H, Han B, Zhang Y, et al: Spectrum-effect relationships between UPLC fingerprints and bioactivities of crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi) and its three processed products on mitochondrial growth coupled with canonical correlation analysis. J Ethnopharmacol. 153:615–623. 2014. View Article : Google Scholar : PubMed/NCBI
12	Shi Z, Liu Z, Liu C, Wu M, Su H, Ma X, Zang Y, Wang J, Zhao Y and Xiao X: Spectrum-effect relationships between chemical fingerprints and antibacterial effects of lonicerae japonicae flos and lonicerae flos base on UPLC and microcalorimetry. Front Pharmacol. 7:122016. View Article : Google Scholar : PubMed/NCBI
13	Chen Y, Yu H, Wu H, Pan Y, Wang K, Liu L, Jin Y and Zhang C: Tracing novel hemostatic compounds from heating products of total flavonoids in Flos Sophorae byspectrum-effect relationships and column chromatography. J Sep Sci. 38:1691–1699. 2015. View Article : Google Scholar : PubMed/NCBI
14	Liu X, Wang XL, Wu L, Li H, Qin KM, Cai H, Pei K, Liu T and Cai BC: Investigation on the spectrum-effect relationships of Da-Huang-Fu-Zi-Tang in rats by UHPLC-ESI-Q-TOF-MS method. J Ethnopharmacol. 154:606–612. 2014. View Article : Google Scholar : PubMed/NCBI
15	Xie RF, Zhou X, Shi ZN, Li YM and Li ZC: Study on spectrum-effect relationship of rhizoma Rhei, cortex Magnoliae Officinalis, fructus Aurantii immaturus and their formula. J Chromatogr Sci. 51:524–532. 2013. View Article : Google Scholar : PubMed/NCBI
16	Ding JX, Li WL, Bai J and Ji YB: Studies on spectrum-effect correlation of mimic estrogenic activity of Cuscuta chinensis. Chin Pharm J. 337–340. 2013.(In Chinese).
17	Sun SL, Guo L, Ren YC, Wang B, Li RH, Qi YS, Yu H, Chang ND, Li MH and Peng HS: Anti-apoptosis effect of polysaccharide isolated from the seeds of Cuscuta chinensis Lam on cardiomyocytes in aging rats. Mol Biol Rep. 41:6117–6124. 2014. View Article : Google Scholar : PubMed/NCBI
18	Kang J, Li X, Geng J, Han L, Tang J, Jin Y and Zhang Y: Determination of hyperin in seed of Cuscuta chinensis Lam. by enhanced chemiluminescence of CdTe quantum dots on calcein/K3Fe(CN)(6) system. Food Chem. 134:2383–2388. 2012. View Article : Google Scholar : PubMed/NCBI
19	Yang L, Chen Q, Wang F and Zhang G: Antiosteoporotic compounds from seeds of Cuscuta chinensis. J Ethnopharmacol. 135:553–560. 2011. View Article : Google Scholar : PubMed/NCBI
20	Liao JC, Chang WT, Lee MS, Chiu YJ, Chao WK, Lin YC, Lin MK and Peng WH: Antinociceptive and anti-inflammatory activities of Cuscuta chinensis seeds in mice. Am J Chin Med. 42:223–242. 2014. View Article : Google Scholar : PubMed/NCBI
21	Yang S, Xu X, Xu H, Xu S, Lin Q, Jia Z, Han T, Zhang H, Zhang Y, Liu H, et al: Purification, characterization and biological effect of reversing the kidney-yang deficiency of polysaccharides from semen cuscutae. Carbohydr Polym. 175:249–256. 2017. View Article : Google Scholar : PubMed/NCBI
22	Zou D, Wang J, Zhang B, Xie S, Wang Q, Xu K and Lin R: Analysis of chemical constituents in Wuzi-Yanzong-Wan by UPLC-ESI-LTQ-Orbitrap-MS. Molecules. 20:21373–21404. 2015. View Article : Google Scholar : PubMed/NCBI
23	Chen ML, Miao L, Cao J, Ip SP and Che CT: Quantitative analysis of biologically active ingredients of five seeds combo by liquid chromatography-quadrupole time-of-flight mass spectrometry for quality control of commercial herbal product. J Sep Sci. 35:1612–1618. 2012. View Article : Google Scholar : PubMed/NCBI
24	Ding JX: Correlation of researches on the fingerprints and estrogenic activity of Cuscuta chinensis. Master's degree thesis of Harbin University of Commerce. 18–19. 2013.(In Chinese).
25	Chatkupt TT, Libal NL, Mader SL, Murphy SJ and Saunders KE: Effect of continuous trio breeding compared with continuous pair breeding in ‘Shoebox’ caging on measures of reproductive performance in Estrogen receptor knockout mice. J Am Assoc Lab Anim Sci. 2018. View Article : Google Scholar :