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Article

Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry

  • Authors:
    • Jing‑Xin Ding
    • Wen‑Lan Li
    • Yang Hu
    • Hui Song
    • Xiang‑Ming Sun
    • Yu‑Bin Ji
  • View Affiliations / Copyright

    Affiliations: Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin, Heilongjiang 150076, P.R. China, College of Pharmacy, Harbin University of Commerce, Harbin, Heilongjiang 150076, P.R. China
  • Pages: 1238-1247
    |
    Published online on: December 12, 2018
       https://doi.org/10.3892/mmr.2018.9755
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Abstract

High-performance liquid chromatography (HPLC) is an efficient method that is widely used to assess the quality of traditional Chinese medicine (TCM). It is well known that the quality of TCM has a direct effect on its efficacy; therefore, in order to thoroughly explain how TCM exerts its efficacy, it is necessary to characterize its active ingredients and assess their quality. The application of the spectrum‑effect method is crucial for determining the pharmacological basis of materials. The aim of the present study was to examine the correlation between chemical spectra and estrogenic activity of Cuscuta chinensis Lam., in order to reveal active compounds with potential therapeutic effects. The spectra of Cuscuta chinensis Lam. were recorded using HPLC, and estrogenic activity was determined using a uterus growth test and MTT assay. Combination of the results of bivariate analysis, principal component analysis and Gray relational analysis identified 19 active compounds, as follows: Quercetin‑3‑O‑(2‘‑O‑α‑rhamnosy‑6’‑O‑malony)‑​β‑D‑glucoside, ka-empferol‑3‑O‑β‑D‑aplosyl‑(1→2)‑​[‑α‑​L‑rhamnosy‑(1→6)]‑β-wD-glucoside, 6‑O‑(E)‑P‑coumaroyl)‑β‑​D‑fructofuranosyl‑(2→1)‑α‑D‑glucopyranoside, kaempferol‑7‑rhamnosy, kaempferol‑3‑β‑D-glucuronide, apigenin, 4‑caffeoyl‑5‑coumaroylquinic acid, kaempferol‑3‑arabofuranoside, quercetin‑3‑O-β‑D-apiofuranosyl-(1→2)-β‑D‑galactoside, dicaffeoylquinic acid, hyperin, quercitin, isorhamnetin, chlorogenic acid, quercetin, quercltrin‑2''‑gallate, quercetin‑3, 7‑α‑L‑dirhamnoside and stigmasterol, as well as one unknown compound. The present study laid a foundation for in vivo metabolic studies regarding Cuscuta chinensis Lam. and for the development of its clinical application.
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Copy and paste a formatted citation
Spandidos Publications style
Ding JX, Li WL, Hu Y, Song H, Sun XM and Ji YB: Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry. Mol Med Rep 19: 1238-1247, 2019.
APA
Ding, J., Li, W., Hu, Y., Song, H., Sun, X., & Ji, Y. (2019). Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry. Molecular Medicine Reports, 19, 1238-1247. https://doi.org/10.3892/mmr.2018.9755
MLA
Ding, J., Li, W., Hu, Y., Song, H., Sun, X., Ji, Y."Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry". Molecular Medicine Reports 19.2 (2019): 1238-1247.
Chicago
Ding, J., Li, W., Hu, Y., Song, H., Sun, X., Ji, Y."Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry". Molecular Medicine Reports 19, no. 2 (2019): 1238-1247. https://doi.org/10.3892/mmr.2018.9755
Copy and paste a formatted citation
x
Spandidos Publications style
Ding JX, Li WL, Hu Y, Song H, Sun XM and Ji YB: Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry. Mol Med Rep 19: 1238-1247, 2019.
APA
Ding, J., Li, W., Hu, Y., Song, H., Sun, X., & Ji, Y. (2019). Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry. Molecular Medicine Reports, 19, 1238-1247. https://doi.org/10.3892/mmr.2018.9755
MLA
Ding, J., Li, W., Hu, Y., Song, H., Sun, X., Ji, Y."Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry". Molecular Medicine Reports 19.2 (2019): 1238-1247.
Chicago
Ding, J., Li, W., Hu, Y., Song, H., Sun, X., Ji, Y."Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high‑performance liquid chromatography/quadrupole time‑of‑flight mass spectrometry". Molecular Medicine Reports 19, no. 2 (2019): 1238-1247. https://doi.org/10.3892/mmr.2018.9755
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