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Article Open Access

Oxidative stress, autophagy and pyroptosis in the neovascularization of oxygen‑induced retinopathy in mice

Erratum in: /10.3892/mmr.2020.11753
  • Authors:
    • Shuai Wang
    • Li‑Yang Ji
    • Li Li
    • Jing‑Min Li
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China, Department of Ophthalmology, Zaozhuang Municipal Hospital, Zaozhuang, Shandong 277000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 927-934
    |
    Published online on: December 13, 2018
       https://doi.org/10.3892/mmr.2018.9759
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Abstract

Retinal neovascularization (RNV) is a principal cause of visual impairment and blindness worldwide. The present study aimed to investigate how oxidative stress, autophagy and pyroptosis alter in RNV. The oxygen‑induced retinopathy (OIR) model was established in C57BL/6J mice by exposing them to a high concentration of oxygen. RNV was clearly visible in the fundus images and was qualitatively analyzed by counting the number of neovascular endothelial cell nuclei at postnatal day 17. Subsequently, the expression of vascular endothelial growth factor (VEGF)‑A and hypoxia‑inducible factor‑1α (HIF‑1α) at the protein level were measured. Furthermore, oxidative stress was examined using dihydroethidium (DHE) staining, and NADPH oxidase (NOX) 1 and 4 in the retinas were detected using reverse transcription‑quantitative polymerase chain reaction analysis. Additionally, immunostaining of microtubule associated protein 1 light chain 3α (LC3) was performed and the expression levels of the LC3, p62, autophagy protein (Atg)5, Atg7, Atg12, Beclin1, NOD‑like receptor family pyrin domain‑containing 3 (NLRP3), caspase‑1, interleukin (IL)‑1β, pro‑caspase‑1 and pro‑IL‑1β proteins were determined using western blotting in order to detect pyroptosis and autophagic flux. Autophagosomes were also detected using transmission electron microscopy. The results revealed that VEGF‑A and HIF‑1α protein expression levels, the DHE‑positive area, and NOX1 and NOX4 mRNA expression levels were significantly increased in the OIR mice. Furthermore, increased levels of NLRP3, caspase‑1, IL‑1β, pro‑caspase‑1 and pro‑IL‑1β proteins demonstrated that pyroptosis was activated. However, an accumulation of p62 and a reduction in the levels of LC3II/I and autophagosomes indicated that autophagic flux was compromised. Therefore, elevated levels of reactive oxygen species and pyroptosis along with attenuated autophagy were demonstrated in the OIR mice. The combination of oxidative stress, pyroptosis and impaired autophagy may serve an important role in the pathophysiology of RNV and may be a potential target to prevent RNV.
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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Ji LY, Li L and Li JM: Oxidative stress, autophagy and pyroptosis in the neovascularization of oxygen‑induced retinopathy in mice Erratum in /10.3892/mmr.2020.11753. Mol Med Rep 19: 927-934, 2019.
APA
Wang, S., Ji, L., Li, L., & Li, J. (2019). Oxidative stress, autophagy and pyroptosis in the neovascularization of oxygen‑induced retinopathy in mice Erratum in /10.3892/mmr.2020.11753. Molecular Medicine Reports, 19, 927-934. https://doi.org/10.3892/mmr.2018.9759
MLA
Wang, S., Ji, L., Li, L., Li, J."Oxidative stress, autophagy and pyroptosis in the neovascularization of oxygen‑induced retinopathy in mice Erratum in /10.3892/mmr.2020.11753". Molecular Medicine Reports 19.2 (2019): 927-934.
Chicago
Wang, S., Ji, L., Li, L., Li, J."Oxidative stress, autophagy and pyroptosis in the neovascularization of oxygen‑induced retinopathy in mice Erratum in /10.3892/mmr.2020.11753". Molecular Medicine Reports 19, no. 2 (2019): 927-934. https://doi.org/10.3892/mmr.2018.9759
Copy and paste a formatted citation
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Spandidos Publications style
Wang S, Ji LY, Li L and Li JM: Oxidative stress, autophagy and pyroptosis in the neovascularization of oxygen‑induced retinopathy in mice Erratum in /10.3892/mmr.2020.11753. Mol Med Rep 19: 927-934, 2019.
APA
Wang, S., Ji, L., Li, L., & Li, J. (2019). Oxidative stress, autophagy and pyroptosis in the neovascularization of oxygen‑induced retinopathy in mice Erratum in /10.3892/mmr.2020.11753. Molecular Medicine Reports, 19, 927-934. https://doi.org/10.3892/mmr.2018.9759
MLA
Wang, S., Ji, L., Li, L., Li, J."Oxidative stress, autophagy and pyroptosis in the neovascularization of oxygen‑induced retinopathy in mice Erratum in /10.3892/mmr.2020.11753". Molecular Medicine Reports 19.2 (2019): 927-934.
Chicago
Wang, S., Ji, L., Li, L., Li, J."Oxidative stress, autophagy and pyroptosis in the neovascularization of oxygen‑induced retinopathy in mice Erratum in /10.3892/mmr.2020.11753". Molecular Medicine Reports 19, no. 2 (2019): 927-934. https://doi.org/10.3892/mmr.2018.9759
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