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Article Open Access

Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes

  • Authors:
    • Zhen Wang
    • Huaping Zheng
    • Hong Zhou
    • Nongyu Huang
    • Xiaoqiong Wei
    • Xiao Liu
    • Xiu Teng
    • Zhonglan Hu
    • Jun Zhang
    • Xikun Zhou
    • Wei Li
    • Jiong Li
  • View Affiliations / Copyright

    Affiliations: Department of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan 610041, P.R. China, Department of Dermatovenereology, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1529-1542
    |
    Published online on: December 20, 2018
       https://doi.org/10.3892/mmr.2018.9782
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Abstract

Psoriasis is a chronic inflammatory skin disease. Keratinocytes (KCs), as skin‑specific cells, serve an important role in the immunopathogenesis of psoriasis. In the present study, transcriptome data derived from psoriasis‑like KCs were used together with the reported transcriptome data from the skin/epidermis of patient with psoriasis, excluding known psoriasis‑associated genes that have been well described in the previous studies according to GeneCards database, to screen for novel psoriasis‑associated genes. According to the human expressed sequence tag of UniGene dataset, six genes that are located near psoriasis‑associated loci were highly expressed in skin. Among these six genes, four genes (epiregulin, NIPA like domain containing 4, serpin family B member 7 and WAP four‑disulfide core domain 12) were highly expressed in normal mouse epidermis (mainly KCs) and mouse psoriatic epidermis cells, but not in psoriatic dermis cells, which further emphasized the specificity of these genes. Furthermore, in systemic inflammatory response syndrome (SIRS), SERPINB7 showed no difference in expression in immune‑activated tissues from SIRS and control mice. It was also found that the mRNA expression levels of SERPINB in lesional skin of patients with psoriasis were significantly higher than in non‑lesional psoriatic skin from the same patients. SERPINB7 may be a valuable candidate for further studies. In the present study, a method for identifying novel key pathogenic skin‑specific molecules is presented, which may be used for investigating and treating psoriasis.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Zheng H, Zhou H, Huang N, Wei X, Liu X, Teng X, Hu Z, Zhang J, Zhou X, Zhou X, et al: Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes. Mol Med Rep 19: 1529-1542, 2019.
APA
Wang, Z., Zheng, H., Zhou, H., Huang, N., Wei, X., Liu, X. ... Li, J. (2019). Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes. Molecular Medicine Reports, 19, 1529-1542. https://doi.org/10.3892/mmr.2018.9782
MLA
Wang, Z., Zheng, H., Zhou, H., Huang, N., Wei, X., Liu, X., Teng, X., Hu, Z., Zhang, J., Zhou, X., Li, W., Li, J."Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes". Molecular Medicine Reports 19.3 (2019): 1529-1542.
Chicago
Wang, Z., Zheng, H., Zhou, H., Huang, N., Wei, X., Liu, X., Teng, X., Hu, Z., Zhang, J., Zhou, X., Li, W., Li, J."Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes". Molecular Medicine Reports 19, no. 3 (2019): 1529-1542. https://doi.org/10.3892/mmr.2018.9782
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Zheng H, Zhou H, Huang N, Wei X, Liu X, Teng X, Hu Z, Zhang J, Zhou X, Zhou X, et al: Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes. Mol Med Rep 19: 1529-1542, 2019.
APA
Wang, Z., Zheng, H., Zhou, H., Huang, N., Wei, X., Liu, X. ... Li, J. (2019). Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes. Molecular Medicine Reports, 19, 1529-1542. https://doi.org/10.3892/mmr.2018.9782
MLA
Wang, Z., Zheng, H., Zhou, H., Huang, N., Wei, X., Liu, X., Teng, X., Hu, Z., Zhang, J., Zhou, X., Li, W., Li, J."Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes". Molecular Medicine Reports 19.3 (2019): 1529-1542.
Chicago
Wang, Z., Zheng, H., Zhou, H., Huang, N., Wei, X., Liu, X., Teng, X., Hu, Z., Zhang, J., Zhou, X., Li, W., Li, J."Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes". Molecular Medicine Reports 19, no. 3 (2019): 1529-1542. https://doi.org/10.3892/mmr.2018.9782
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